Influence of progesterone supplementation during the first third of pregnancy on fetal and placental growth in overnourished adolescent ewes

Jacqueline Wallace, D A Bourke, P Da Silva, Raymond Aitken

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Overnourishing adolescent ewes throughout pregnancy promotes maternal tissue synthesis at the expense of placental growth, which in turn leads to a major decrease in lamb birth weight. As maternal dietary intakes are inversely related to peripheral progesterone concentrations in these adolescent dams, it was hypothesized that sup-optimal progesterone concentrations in overnourished dams may compromise the growth of the differentiating conceptus resulting in fewer uterine caruncles being occupied and, hence, fewer placentomes formed. This hypothesis was tested by supplementing overnourished adolescent dams with exogenous progesterone during early pregnancy and determining the impact on pregnancy outcome at term. Embryos recovered from superovulated adult ewes inseminated by a single sire were transferred in singleton to the uterus of peripubertal adolescent recipients. After transfer of embryos, ewes were offered a moderate or high amount of a complete diet (n = 11 per group). A further high intake group received a progesterone supplement each day from day 5 to day 55 of gestation (term = 145 days) to restore circulating progesterone concentrations to moderate values throughout the first third of pregnancy (n = 11). For ewes establishing pregnancies (n = 7 per group), live weight gain during the first 100 days of gestation was 66 +/- 4, 323 +/- 17 and 300 +/- 7 g per day, body condition score at term was 2.1 +/- 0.05, 3.0 +/- 0.08 and 3.1 +/- 0.07 units and the duration of gestation after spontaneous delivery was 148 +/- 1.7, 144 +/- 0.8 and 143 +/- 0.8 days for the moderate intake, high intake and high intake plus progesterone groups, respectively. At delivery, fetal cotyledon mass (136 +/- 12.1 versus 57 +/- 8.2 g, P < 0.001) and lamb birth weight (5164 +/- 151 versus 2893 +/- 381 g, P < 0.001) were higher in moderate intake than in high intake dams. Progesterone supplementation restored circulating concentrations to moderate values during the first third of gestation. Lamb birth weight in the high intake plus progesterone group (4150 +/- 389 g) was intermediate between the high intake (P < 0.02) and moderate intake (P < 0.05) groups, but this change in birth weight was not associated with corresponding changes in fetal cotyledon mass (76 +/- 10.3 g). Moreover, the number of fetal cotyledons was similar in all three groups. Thus, progesterone did not directly affect the growth of the fetal cotyledon but may have influenced placental vascularity, blood flow or nutrient transfer capacity or alternatively the development of the embryonic inner cell mass.

Original languageEnglish
Pages (from-to)481-487
Number of pages7
JournalReproduction
Volume126
Issue number4
DOIs
Publication statusPublished - Oct 2003

Keywords

  • sheep administered progesterone
  • carrying singleton fetuses
  • cattle

Cite this

Influence of progesterone supplementation during the first third of pregnancy on fetal and placental growth in overnourished adolescent ewes. / Wallace, Jacqueline; Bourke, D A ; Da Silva, P ; Aitken, Raymond.

In: Reproduction, Vol. 126, No. 4, 10.2003, p. 481-487.

Research output: Contribution to journalArticle

@article{a4ecb8bf8f9a4cf0b3b19729ad1d824d,
title = "Influence of progesterone supplementation during the first third of pregnancy on fetal and placental growth in overnourished adolescent ewes",
abstract = "Overnourishing adolescent ewes throughout pregnancy promotes maternal tissue synthesis at the expense of placental growth, which in turn leads to a major decrease in lamb birth weight. As maternal dietary intakes are inversely related to peripheral progesterone concentrations in these adolescent dams, it was hypothesized that sup-optimal progesterone concentrations in overnourished dams may compromise the growth of the differentiating conceptus resulting in fewer uterine caruncles being occupied and, hence, fewer placentomes formed. This hypothesis was tested by supplementing overnourished adolescent dams with exogenous progesterone during early pregnancy and determining the impact on pregnancy outcome at term. Embryos recovered from superovulated adult ewes inseminated by a single sire were transferred in singleton to the uterus of peripubertal adolescent recipients. After transfer of embryos, ewes were offered a moderate or high amount of a complete diet (n = 11 per group). A further high intake group received a progesterone supplement each day from day 5 to day 55 of gestation (term = 145 days) to restore circulating progesterone concentrations to moderate values throughout the first third of pregnancy (n = 11). For ewes establishing pregnancies (n = 7 per group), live weight gain during the first 100 days of gestation was 66 +/- 4, 323 +/- 17 and 300 +/- 7 g per day, body condition score at term was 2.1 +/- 0.05, 3.0 +/- 0.08 and 3.1 +/- 0.07 units and the duration of gestation after spontaneous delivery was 148 +/- 1.7, 144 +/- 0.8 and 143 +/- 0.8 days for the moderate intake, high intake and high intake plus progesterone groups, respectively. At delivery, fetal cotyledon mass (136 +/- 12.1 versus 57 +/- 8.2 g, P < 0.001) and lamb birth weight (5164 +/- 151 versus 2893 +/- 381 g, P < 0.001) were higher in moderate intake than in high intake dams. Progesterone supplementation restored circulating concentrations to moderate values during the first third of gestation. Lamb birth weight in the high intake plus progesterone group (4150 +/- 389 g) was intermediate between the high intake (P < 0.02) and moderate intake (P < 0.05) groups, but this change in birth weight was not associated with corresponding changes in fetal cotyledon mass (76 +/- 10.3 g). Moreover, the number of fetal cotyledons was similar in all three groups. Thus, progesterone did not directly affect the growth of the fetal cotyledon but may have influenced placental vascularity, blood flow or nutrient transfer capacity or alternatively the development of the embryonic inner cell mass.",
keywords = "sheep administered progesterone, carrying singleton fetuses, cattle",
author = "Jacqueline Wallace and Bourke, {D A} and {Da Silva}, P and Raymond Aitken",
year = "2003",
month = "10",
doi = "10.1530/rep.0.1260481",
language = "English",
volume = "126",
pages = "481--487",
journal = "Reproduction",
issn = "1470-1626",
publisher = "BioScientifica Ltd.",
number = "4",

}

TY - JOUR

T1 - Influence of progesterone supplementation during the first third of pregnancy on fetal and placental growth in overnourished adolescent ewes

AU - Wallace, Jacqueline

AU - Bourke, D A

AU - Da Silva, P

AU - Aitken, Raymond

PY - 2003/10

Y1 - 2003/10

N2 - Overnourishing adolescent ewes throughout pregnancy promotes maternal tissue synthesis at the expense of placental growth, which in turn leads to a major decrease in lamb birth weight. As maternal dietary intakes are inversely related to peripheral progesterone concentrations in these adolescent dams, it was hypothesized that sup-optimal progesterone concentrations in overnourished dams may compromise the growth of the differentiating conceptus resulting in fewer uterine caruncles being occupied and, hence, fewer placentomes formed. This hypothesis was tested by supplementing overnourished adolescent dams with exogenous progesterone during early pregnancy and determining the impact on pregnancy outcome at term. Embryos recovered from superovulated adult ewes inseminated by a single sire were transferred in singleton to the uterus of peripubertal adolescent recipients. After transfer of embryos, ewes were offered a moderate or high amount of a complete diet (n = 11 per group). A further high intake group received a progesterone supplement each day from day 5 to day 55 of gestation (term = 145 days) to restore circulating progesterone concentrations to moderate values throughout the first third of pregnancy (n = 11). For ewes establishing pregnancies (n = 7 per group), live weight gain during the first 100 days of gestation was 66 +/- 4, 323 +/- 17 and 300 +/- 7 g per day, body condition score at term was 2.1 +/- 0.05, 3.0 +/- 0.08 and 3.1 +/- 0.07 units and the duration of gestation after spontaneous delivery was 148 +/- 1.7, 144 +/- 0.8 and 143 +/- 0.8 days for the moderate intake, high intake and high intake plus progesterone groups, respectively. At delivery, fetal cotyledon mass (136 +/- 12.1 versus 57 +/- 8.2 g, P < 0.001) and lamb birth weight (5164 +/- 151 versus 2893 +/- 381 g, P < 0.001) were higher in moderate intake than in high intake dams. Progesterone supplementation restored circulating concentrations to moderate values during the first third of gestation. Lamb birth weight in the high intake plus progesterone group (4150 +/- 389 g) was intermediate between the high intake (P < 0.02) and moderate intake (P < 0.05) groups, but this change in birth weight was not associated with corresponding changes in fetal cotyledon mass (76 +/- 10.3 g). Moreover, the number of fetal cotyledons was similar in all three groups. Thus, progesterone did not directly affect the growth of the fetal cotyledon but may have influenced placental vascularity, blood flow or nutrient transfer capacity or alternatively the development of the embryonic inner cell mass.

AB - Overnourishing adolescent ewes throughout pregnancy promotes maternal tissue synthesis at the expense of placental growth, which in turn leads to a major decrease in lamb birth weight. As maternal dietary intakes are inversely related to peripheral progesterone concentrations in these adolescent dams, it was hypothesized that sup-optimal progesterone concentrations in overnourished dams may compromise the growth of the differentiating conceptus resulting in fewer uterine caruncles being occupied and, hence, fewer placentomes formed. This hypothesis was tested by supplementing overnourished adolescent dams with exogenous progesterone during early pregnancy and determining the impact on pregnancy outcome at term. Embryos recovered from superovulated adult ewes inseminated by a single sire were transferred in singleton to the uterus of peripubertal adolescent recipients. After transfer of embryos, ewes were offered a moderate or high amount of a complete diet (n = 11 per group). A further high intake group received a progesterone supplement each day from day 5 to day 55 of gestation (term = 145 days) to restore circulating progesterone concentrations to moderate values throughout the first third of pregnancy (n = 11). For ewes establishing pregnancies (n = 7 per group), live weight gain during the first 100 days of gestation was 66 +/- 4, 323 +/- 17 and 300 +/- 7 g per day, body condition score at term was 2.1 +/- 0.05, 3.0 +/- 0.08 and 3.1 +/- 0.07 units and the duration of gestation after spontaneous delivery was 148 +/- 1.7, 144 +/- 0.8 and 143 +/- 0.8 days for the moderate intake, high intake and high intake plus progesterone groups, respectively. At delivery, fetal cotyledon mass (136 +/- 12.1 versus 57 +/- 8.2 g, P < 0.001) and lamb birth weight (5164 +/- 151 versus 2893 +/- 381 g, P < 0.001) were higher in moderate intake than in high intake dams. Progesterone supplementation restored circulating concentrations to moderate values during the first third of gestation. Lamb birth weight in the high intake plus progesterone group (4150 +/- 389 g) was intermediate between the high intake (P < 0.02) and moderate intake (P < 0.05) groups, but this change in birth weight was not associated with corresponding changes in fetal cotyledon mass (76 +/- 10.3 g). Moreover, the number of fetal cotyledons was similar in all three groups. Thus, progesterone did not directly affect the growth of the fetal cotyledon but may have influenced placental vascularity, blood flow or nutrient transfer capacity or alternatively the development of the embryonic inner cell mass.

KW - sheep administered progesterone

KW - carrying singleton fetuses

KW - cattle

U2 - 10.1530/rep.0.1260481

DO - 10.1530/rep.0.1260481

M3 - Article

VL - 126

SP - 481

EP - 487

JO - Reproduction

JF - Reproduction

SN - 1470-1626

IS - 4

ER -