Influence of soil type and pH on the colonisation of sugar beet seedlings by antagonistic Pseudomonas and Bacillus strains, and on their control of Pythium damping-off

C. S. Schmidt, F. Agostini, J. Whyte, John Andrew Townend, C. Leifert, Kenneth Stuart Killham, Christopher Mullins

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In five different soils originating from Scotland (Craibstone and Cruden Bay), Germany ( Magdeburg and Uelzen) and Greece (Tymbaki), Pseudomonas fluorescens B5 reached higher population sizes (4.7-5.7 log CFU/plant) on 12-day-old sugar beet seedlings than Bacillus subtilis MBI 600 (4.1 - 4.8 log CFU/ plant). Total population size per plant was not affected by soil type. In all five soils, the antagonists reached highest population densities in the hypocotyl and the upper 2 cm root section (P. fluorescens B5: 5.2-6.8 log(10) CFU/g plant fresh weight, Bacillus subtilis MBI 600: 5.2 - 6.1 log(10) CFU/g plant fresh weight) and declined to 0 - 3 log(10) CFU below 4 cm root depth. Colonisation by P. fluorescens B5 down the root was slightly increased in the soils from Craibstone, Magdeburg, and Uelzen compared to the sandy clay loam from Tymbaki. In lux-marked P. fluorescens B5, population density was positively correlated with light emission in all soils; the light emission indicated physiological activity of the strains. However, P. fluorescens B5 reduced Pythium damping-off ( measurement after 14 days plant growth) only in three of the five soils ( Craibstone, Cruden Bay and Magdeburg). Co-inoculation of B. subtilis MBI 600 increased downward colonisation of the root by P. fluorescens B5, but not the total population of P. fluorescens B5 per plant. Bacillus subtilis MBI 600 did not reduce Pythium damping-off in any of the soils nor did it influence the efficiency of co-inoculated P. fluorescens B5; its population consisted mainly of physiologically inactive spores. In Craibstone soil, pH did not affect population density, distribution along the root or biocontrol activity against P. ultimum of P. fluorescens B5 or B. subtilis MBI 600.

Original languageEnglish
Pages (from-to)1025-1046
Number of pages21
JournalEuropean Journal of Plant Pathology
Volume110
DOIs
Publication statusPublished - 2004

Keywords

  • biological control
  • bioluminescence
  • lux-marking
  • rhizosphere
  • root colonisation
  • soil-borne diseases
  • GRAMINIS VAR TRITICI
  • SUPPRESS TAKE-ALL
  • BIOLOGICAL-CONTROL
  • ROOT COLONIZATION
  • FLUORESCENT PSEUDOMONADS
  • BIOCONTROL AGENT
  • LIQUID CULTURE
  • FUSARIUM-WILT
  • IN-VITRO
  • RHIZOSPHERE

Cite this

Influence of soil type and pH on the colonisation of sugar beet seedlings by antagonistic Pseudomonas and Bacillus strains, and on their control of Pythium damping-off. / Schmidt, C. S.; Agostini, F.; Whyte, J.; Townend, John Andrew; Leifert, C.; Killham, Kenneth Stuart; Mullins, Christopher.

In: European Journal of Plant Pathology, Vol. 110, 2004, p. 1025-1046.

Research output: Contribution to journalArticle

Schmidt, C. S. ; Agostini, F. ; Whyte, J. ; Townend, John Andrew ; Leifert, C. ; Killham, Kenneth Stuart ; Mullins, Christopher. / Influence of soil type and pH on the colonisation of sugar beet seedlings by antagonistic Pseudomonas and Bacillus strains, and on their control of Pythium damping-off. In: European Journal of Plant Pathology. 2004 ; Vol. 110. pp. 1025-1046.
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abstract = "In five different soils originating from Scotland (Craibstone and Cruden Bay), Germany ( Magdeburg and Uelzen) and Greece (Tymbaki), Pseudomonas fluorescens B5 reached higher population sizes (4.7-5.7 log CFU/plant) on 12-day-old sugar beet seedlings than Bacillus subtilis MBI 600 (4.1 - 4.8 log CFU/ plant). Total population size per plant was not affected by soil type. In all five soils, the antagonists reached highest population densities in the hypocotyl and the upper 2 cm root section (P. fluorescens B5: 5.2-6.8 log(10) CFU/g plant fresh weight, Bacillus subtilis MBI 600: 5.2 - 6.1 log(10) CFU/g plant fresh weight) and declined to 0 - 3 log(10) CFU below 4 cm root depth. Colonisation by P. fluorescens B5 down the root was slightly increased in the soils from Craibstone, Magdeburg, and Uelzen compared to the sandy clay loam from Tymbaki. In lux-marked P. fluorescens B5, population density was positively correlated with light emission in all soils; the light emission indicated physiological activity of the strains. However, P. fluorescens B5 reduced Pythium damping-off ( measurement after 14 days plant growth) only in three of the five soils ( Craibstone, Cruden Bay and Magdeburg). Co-inoculation of B. subtilis MBI 600 increased downward colonisation of the root by P. fluorescens B5, but not the total population of P. fluorescens B5 per plant. Bacillus subtilis MBI 600 did not reduce Pythium damping-off in any of the soils nor did it influence the efficiency of co-inoculated P. fluorescens B5; its population consisted mainly of physiologically inactive spores. In Craibstone soil, pH did not affect population density, distribution along the root or biocontrol activity against P. ultimum of P. fluorescens B5 or B. subtilis MBI 600.",
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T1 - Influence of soil type and pH on the colonisation of sugar beet seedlings by antagonistic Pseudomonas and Bacillus strains, and on their control of Pythium damping-off

AU - Schmidt, C. S.

AU - Agostini, F.

AU - Whyte, J.

AU - Townend, John Andrew

AU - Leifert, C.

AU - Killham, Kenneth Stuart

AU - Mullins, Christopher

PY - 2004

Y1 - 2004

N2 - In five different soils originating from Scotland (Craibstone and Cruden Bay), Germany ( Magdeburg and Uelzen) and Greece (Tymbaki), Pseudomonas fluorescens B5 reached higher population sizes (4.7-5.7 log CFU/plant) on 12-day-old sugar beet seedlings than Bacillus subtilis MBI 600 (4.1 - 4.8 log CFU/ plant). Total population size per plant was not affected by soil type. In all five soils, the antagonists reached highest population densities in the hypocotyl and the upper 2 cm root section (P. fluorescens B5: 5.2-6.8 log(10) CFU/g plant fresh weight, Bacillus subtilis MBI 600: 5.2 - 6.1 log(10) CFU/g plant fresh weight) and declined to 0 - 3 log(10) CFU below 4 cm root depth. Colonisation by P. fluorescens B5 down the root was slightly increased in the soils from Craibstone, Magdeburg, and Uelzen compared to the sandy clay loam from Tymbaki. In lux-marked P. fluorescens B5, population density was positively correlated with light emission in all soils; the light emission indicated physiological activity of the strains. However, P. fluorescens B5 reduced Pythium damping-off ( measurement after 14 days plant growth) only in three of the five soils ( Craibstone, Cruden Bay and Magdeburg). Co-inoculation of B. subtilis MBI 600 increased downward colonisation of the root by P. fluorescens B5, but not the total population of P. fluorescens B5 per plant. Bacillus subtilis MBI 600 did not reduce Pythium damping-off in any of the soils nor did it influence the efficiency of co-inoculated P. fluorescens B5; its population consisted mainly of physiologically inactive spores. In Craibstone soil, pH did not affect population density, distribution along the root or biocontrol activity against P. ultimum of P. fluorescens B5 or B. subtilis MBI 600.

AB - In five different soils originating from Scotland (Craibstone and Cruden Bay), Germany ( Magdeburg and Uelzen) and Greece (Tymbaki), Pseudomonas fluorescens B5 reached higher population sizes (4.7-5.7 log CFU/plant) on 12-day-old sugar beet seedlings than Bacillus subtilis MBI 600 (4.1 - 4.8 log CFU/ plant). Total population size per plant was not affected by soil type. In all five soils, the antagonists reached highest population densities in the hypocotyl and the upper 2 cm root section (P. fluorescens B5: 5.2-6.8 log(10) CFU/g plant fresh weight, Bacillus subtilis MBI 600: 5.2 - 6.1 log(10) CFU/g plant fresh weight) and declined to 0 - 3 log(10) CFU below 4 cm root depth. Colonisation by P. fluorescens B5 down the root was slightly increased in the soils from Craibstone, Magdeburg, and Uelzen compared to the sandy clay loam from Tymbaki. In lux-marked P. fluorescens B5, population density was positively correlated with light emission in all soils; the light emission indicated physiological activity of the strains. However, P. fluorescens B5 reduced Pythium damping-off ( measurement after 14 days plant growth) only in three of the five soils ( Craibstone, Cruden Bay and Magdeburg). Co-inoculation of B. subtilis MBI 600 increased downward colonisation of the root by P. fluorescens B5, but not the total population of P. fluorescens B5 per plant. Bacillus subtilis MBI 600 did not reduce Pythium damping-off in any of the soils nor did it influence the efficiency of co-inoculated P. fluorescens B5; its population consisted mainly of physiologically inactive spores. In Craibstone soil, pH did not affect population density, distribution along the root or biocontrol activity against P. ultimum of P. fluorescens B5 or B. subtilis MBI 600.

KW - biological control

KW - bioluminescence

KW - lux-marking

KW - rhizosphere

KW - root colonisation

KW - soil-borne diseases

KW - GRAMINIS VAR TRITICI

KW - SUPPRESS TAKE-ALL

KW - BIOLOGICAL-CONTROL

KW - ROOT COLONIZATION

KW - FLUORESCENT PSEUDOMONADS

KW - BIOCONTROL AGENT

KW - LIQUID CULTURE

KW - FUSARIUM-WILT

KW - IN-VITRO

KW - RHIZOSPHERE

U2 - 10.1007/s10658-004-1600-y

DO - 10.1007/s10658-004-1600-y

M3 - Article

VL - 110

SP - 1025

EP - 1046

JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

SN - 0929-1873

ER -