Inhibition of rainbow trout phagocyte responsiveness tohuman tumor necrosis factor a(hTNFa) with monoclonal antibodies to the hTNFa 55 kDa receptor

S. I. Jang, V. Mulero, L. J. Hardie, C. J. Secombes*

*Corresponding author for this work

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Human tumor necrosis factor a (hTNFa) was found to increase rainbow trout macrophage respiratory burst activity and neutrophil migration. The increase in respiratory burst activity was inhibited by preincubation of the hTNFa with a soluble 55 kDa hTNFa receptor (TNF-Rl). Preincubation of the phagocytes with monoclonal antibodies (MAbs) to the TNF-R1 was found to ablate their responsiveness to hTNFa, dependent on the concentration of MAbs used. These findings are discussed with a view towards using such MAbs to elucidate the possible secretion of TNF by fish leucocytes.

Original languageEnglish
Pages (from-to)61-69
Number of pages9
JournalFish and Shellfish Immunology
Volume5
Issue number1
DOIs
Publication statusPublished - Jan 1995

Keywords

  • migration
  • monoclonal antibodies
  • phagocytes
  • rainbow trout
  • respiratory burst
  • tumor necrosis factor a
  • tumor necrosis factor a receptor

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