Inhibition of rainbow trout phagocyte responsiveness tohuman tumor necrosis factor a(hTNFa) with monoclonal antibodies to the hTNFa 55 kDa receptor

S. I. Jang; V. Mulero; L. J. Hardie; C. J. Secombes

doi:10.1016/S1050-4648(05)80007-5

Inhibition of rainbow trout phagocyte responsiveness tohuman tumor necrosis factor a(hTNFa) with monoclonal antibodies to the hTNFa 55 kDa receptor

S. I. Jang, V. Mulero, L. J. Hardie, C. J. Secombes^*

^*Corresponding author for this work

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Human tumor necrosis factor a (hTNFa) was found to increase rainbow trout macrophage respiratory burst activity and neutrophil migration. The increase in respiratory burst activity was inhibited by preincubation of the hTNFa with a soluble 55 kDa hTNFa receptor (TNF-Rl). Preincubation of the phagocytes with monoclonal antibodies (MAbs) to the TNF-R1 was found to ablate their responsiveness to hTNFa, dependent on the concentration of MAbs used. These findings are discussed with a view towards using such MAbs to elucidate the possible secretion of TNF by fish leucocytes.

Original language	English
Pages (from-to)	61-69
Number of pages	9
Journal	Fish and Shellfish Immunology
Volume	5
Issue number	1
DOIs	https://doi.org/10.1016/S1050-4648(05)80007-5
Publication status	Published - Jan 1995

Keywords

migration
monoclonal antibodies
phagocytes
rainbow trout
respiratory burst
tumor necrosis factor a
tumor necrosis factor a receptor

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title = "Inhibition of rainbow trout phagocyte responsiveness tohuman tumor necrosis factor a(hTNFa) with monoclonal antibodies to the hTNFa 55 kDa receptor",

abstract = "Human tumor necrosis factor a (hTNFa) was found to increase rainbow trout macrophage respiratory burst activity and neutrophil migration. The increase in respiratory burst activity was inhibited by preincubation of the hTNFa with a soluble 55 kDa hTNFa receptor (TNF-Rl). Preincubation of the phagocytes with monoclonal antibodies (MAbs) to the TNF-R1 was found to ablate their responsiveness to hTNFa, dependent on the concentration of MAbs used. These findings are discussed with a view towards using such MAbs to elucidate the possible secretion of TNF by fish leucocytes.",

keywords = "migration, monoclonal antibodies, phagocytes, rainbow trout, respiratory burst, tumor necrosis factor a, tumor necrosis factor a receptor",

author = "Jang, {S. I.} and V. Mulero and Hardie, {L. J.} and Secombes, {C. J.}",

year = "1995",

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AU - Mulero, V.

AU - Hardie, L. J.

AU - Secombes, C. J.

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N2 - Human tumor necrosis factor a (hTNFa) was found to increase rainbow trout macrophage respiratory burst activity and neutrophil migration. The increase in respiratory burst activity was inhibited by preincubation of the hTNFa with a soluble 55 kDa hTNFa receptor (TNF-Rl). Preincubation of the phagocytes with monoclonal antibodies (MAbs) to the TNF-R1 was found to ablate their responsiveness to hTNFa, dependent on the concentration of MAbs used. These findings are discussed with a view towards using such MAbs to elucidate the possible secretion of TNF by fish leucocytes.

AB - Human tumor necrosis factor a (hTNFa) was found to increase rainbow trout macrophage respiratory burst activity and neutrophil migration. The increase in respiratory burst activity was inhibited by preincubation of the hTNFa with a soluble 55 kDa hTNFa receptor (TNF-Rl). Preincubation of the phagocytes with monoclonal antibodies (MAbs) to the TNF-R1 was found to ablate their responsiveness to hTNFa, dependent on the concentration of MAbs used. These findings are discussed with a view towards using such MAbs to elucidate the possible secretion of TNF by fish leucocytes.

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KW - monoclonal antibodies

KW - phagocytes

KW - rainbow trout

KW - respiratory burst

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KW - tumor necrosis factor a receptor

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Inhibition of rainbow trout phagocyte responsiveness tohuman tumor necrosis factor a(hTNFa) with monoclonal antibodies to the hTNFa 55 kDa receptor

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