Inhibitory effect of caffeic acid phenethyl ester, a plant-derived polyphenolic compound, on rat intestinal contractility

Gabriella Aviello, Caterina Scalisi, Rosaria Fileccia, Raffaele Capasso, Barbara Romano, Angelo A Izzo, Francesca Borrelli

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Caffeic acid phenethyl ester (CAPE) exerts pharmacological actions (e.g. anti-inflammatory, chemopreventive) which are relevant for potential clinical application in the digestive tract. However, no study has been published on its possible effects on intestinal motility, to date. In the present study, we investigated the effect of this plant-derived polyphenolic compound on the spontaneous contractions of the rat isolated ileum. CAPE reduced (in a tetrodotoxin-insensitive manner) spontaneous ileal contractions and this effect was reduced by the L-type Ca2+ channel blocker nifedipine and the chelant of calcium ethylenediaminetetraacetic acid. However, the effect of CAPE was not modified by a number of inhibitors/antagonists such as of phentolamine plus propranolol, atropine, tetrodotoxin, cyclopiazonic acid, omega-conotoxin, apamin, NG-nitro-L-arginine methyl ester, 3-isobutyl-1-methylxanthine, 9-(tetrahydro-2-furanyl)-9H-purin-6-amine, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one or a combination of SR 140333, SR48968 and SR142801. In conclusion our study shows that (i) CAPE relaxed myogenic contractions of rat ileum and that (ii) this effect occurs, at least in part, throughout a mechanism involving L-type Ca2+ channels.

Original languageEnglish
Pages (from-to)163-7
Number of pages5
JournalEuropean Journal of Pharmacology
Volume640
Issue number1-3
Early online date6 May 2010
DOIs
Publication statusPublished - 25 Aug 2010

Fingerprint

Phytochemicals
Tetrodotoxin
Ileum
omega-Conotoxins
Apamin
1-Methyl-3-isobutylxanthine
Gastrointestinal Motility
Phentolamine
NG-Nitroarginine Methyl Ester
Nifedipine
Atropine
Propranolol
Edetic Acid
Gastrointestinal Tract
Anti-Inflammatory Agents
Pharmacology
Calcium
caffeic acid phenethyl ester

Keywords

  • Animals
  • Caffeic Acids
  • Flavonoids
  • Ileum
  • In Vitro Techniques
  • Male
  • Muscle Contraction
  • Muscle Relaxation
  • Phenols
  • Phenylethyl Alcohol
  • Plants
  • Polyphenols
  • Potassium Chloride
  • Rats
  • Rats, Wistar
  • Journal Article

Cite this

Inhibitory effect of caffeic acid phenethyl ester, a plant-derived polyphenolic compound, on rat intestinal contractility. / Aviello, Gabriella; Scalisi, Caterina; Fileccia, Rosaria; Capasso, Raffaele; Romano, Barbara; Izzo, Angelo A; Borrelli, Francesca.

In: European Journal of Pharmacology, Vol. 640, No. 1-3, 25.08.2010, p. 163-7.

Research output: Contribution to journalArticle

Aviello, Gabriella ; Scalisi, Caterina ; Fileccia, Rosaria ; Capasso, Raffaele ; Romano, Barbara ; Izzo, Angelo A ; Borrelli, Francesca. / Inhibitory effect of caffeic acid phenethyl ester, a plant-derived polyphenolic compound, on rat intestinal contractility. In: European Journal of Pharmacology. 2010 ; Vol. 640, No. 1-3. pp. 163-7.
@article{b8f99fbafbce4806b74101ea4ede7880,
title = "Inhibitory effect of caffeic acid phenethyl ester, a plant-derived polyphenolic compound, on rat intestinal contractility",
abstract = "Caffeic acid phenethyl ester (CAPE) exerts pharmacological actions (e.g. anti-inflammatory, chemopreventive) which are relevant for potential clinical application in the digestive tract. However, no study has been published on its possible effects on intestinal motility, to date. In the present study, we investigated the effect of this plant-derived polyphenolic compound on the spontaneous contractions of the rat isolated ileum. CAPE reduced (in a tetrodotoxin-insensitive manner) spontaneous ileal contractions and this effect was reduced by the L-type Ca2+ channel blocker nifedipine and the chelant of calcium ethylenediaminetetraacetic acid. However, the effect of CAPE was not modified by a number of inhibitors/antagonists such as of phentolamine plus propranolol, atropine, tetrodotoxin, cyclopiazonic acid, omega-conotoxin, apamin, NG-nitro-L-arginine methyl ester, 3-isobutyl-1-methylxanthine, 9-(tetrahydro-2-furanyl)-9H-purin-6-amine, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one or a combination of SR 140333, SR48968 and SR142801. In conclusion our study shows that (i) CAPE relaxed myogenic contractions of rat ileum and that (ii) this effect occurs, at least in part, throughout a mechanism involving L-type Ca2+ channels.",
keywords = "Animals, Caffeic Acids, Flavonoids, Ileum, In Vitro Techniques, Male, Muscle Contraction, Muscle Relaxation, Phenols, Phenylethyl Alcohol, Plants, Polyphenols, Potassium Chloride, Rats, Rats, Wistar, Journal Article",
author = "Gabriella Aviello and Caterina Scalisi and Rosaria Fileccia and Raffaele Capasso and Barbara Romano and Izzo, {Angelo A} and Francesca Borrelli",
note = "This study was in part supported by the Regione Campania and the ‘Fondazione Enrico and Enrica Sovena’",
year = "2010",
month = "8",
day = "25",
doi = "10.1016/j.ejphar.2010.04.040",
language = "English",
volume = "640",
pages = "163--7",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Inhibitory effect of caffeic acid phenethyl ester, a plant-derived polyphenolic compound, on rat intestinal contractility

AU - Aviello, Gabriella

AU - Scalisi, Caterina

AU - Fileccia, Rosaria

AU - Capasso, Raffaele

AU - Romano, Barbara

AU - Izzo, Angelo A

AU - Borrelli, Francesca

N1 - This study was in part supported by the Regione Campania and the ‘Fondazione Enrico and Enrica Sovena’

PY - 2010/8/25

Y1 - 2010/8/25

N2 - Caffeic acid phenethyl ester (CAPE) exerts pharmacological actions (e.g. anti-inflammatory, chemopreventive) which are relevant for potential clinical application in the digestive tract. However, no study has been published on its possible effects on intestinal motility, to date. In the present study, we investigated the effect of this plant-derived polyphenolic compound on the spontaneous contractions of the rat isolated ileum. CAPE reduced (in a tetrodotoxin-insensitive manner) spontaneous ileal contractions and this effect was reduced by the L-type Ca2+ channel blocker nifedipine and the chelant of calcium ethylenediaminetetraacetic acid. However, the effect of CAPE was not modified by a number of inhibitors/antagonists such as of phentolamine plus propranolol, atropine, tetrodotoxin, cyclopiazonic acid, omega-conotoxin, apamin, NG-nitro-L-arginine methyl ester, 3-isobutyl-1-methylxanthine, 9-(tetrahydro-2-furanyl)-9H-purin-6-amine, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one or a combination of SR 140333, SR48968 and SR142801. In conclusion our study shows that (i) CAPE relaxed myogenic contractions of rat ileum and that (ii) this effect occurs, at least in part, throughout a mechanism involving L-type Ca2+ channels.

AB - Caffeic acid phenethyl ester (CAPE) exerts pharmacological actions (e.g. anti-inflammatory, chemopreventive) which are relevant for potential clinical application in the digestive tract. However, no study has been published on its possible effects on intestinal motility, to date. In the present study, we investigated the effect of this plant-derived polyphenolic compound on the spontaneous contractions of the rat isolated ileum. CAPE reduced (in a tetrodotoxin-insensitive manner) spontaneous ileal contractions and this effect was reduced by the L-type Ca2+ channel blocker nifedipine and the chelant of calcium ethylenediaminetetraacetic acid. However, the effect of CAPE was not modified by a number of inhibitors/antagonists such as of phentolamine plus propranolol, atropine, tetrodotoxin, cyclopiazonic acid, omega-conotoxin, apamin, NG-nitro-L-arginine methyl ester, 3-isobutyl-1-methylxanthine, 9-(tetrahydro-2-furanyl)-9H-purin-6-amine, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one or a combination of SR 140333, SR48968 and SR142801. In conclusion our study shows that (i) CAPE relaxed myogenic contractions of rat ileum and that (ii) this effect occurs, at least in part, throughout a mechanism involving L-type Ca2+ channels.

KW - Animals

KW - Caffeic Acids

KW - Flavonoids

KW - Ileum

KW - In Vitro Techniques

KW - Male

KW - Muscle Contraction

KW - Muscle Relaxation

KW - Phenols

KW - Phenylethyl Alcohol

KW - Plants

KW - Polyphenols

KW - Potassium Chloride

KW - Rats

KW - Rats, Wistar

KW - Journal Article

U2 - 10.1016/j.ejphar.2010.04.040

DO - 10.1016/j.ejphar.2010.04.040

M3 - Article

C2 - 20451513

VL - 640

SP - 163

EP - 167

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 1-3

ER -