Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters

S C  Campbell; H  Cragg; L J  Elrick; W M  Macfarlane; K I J  Shennan; K  Docherty

Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters

S C Campbell, H Cragg, L J Elrick, W M Macfarlane, K I J Shennan, K Docherty

Medical Sciences

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic beta-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4, The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH-terminus, Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at -253 to -244, and the islet amyloid poly peptide promoter through a sequence element located downstream of -138, The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2-230 and 231-349. (C) 1999 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	53-57
Number of pages	5
Journal	FEBS Letters
Volume	463
Publication status	Published - 1999

Keywords

insulin gene
diabetes mellitus
amylin
transcription
BETA-CELL LINE
MOLECULAR-CLONING
MAMMALIAN-CELLS
PAIRED DOMAIN
GENE
TRANSCRIPTION
IDENTIFICATION
ACTIVATION
SEQUENCE
PROTEIN

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters",

abstract = "Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic beta-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4, The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH-terminus, Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at -253 to -244, and the islet amyloid poly peptide promoter through a sequence element located downstream of -138, The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2-230 and 231-349. (C) 1999 Federation of European Biochemical Societies.",

keywords = "insulin gene, diabetes mellitus, amylin, transcription, BETA-CELL LINE, MOLECULAR-CLONING, MAMMALIAN-CELLS, PAIRED DOMAIN, GENE, TRANSCRIPTION, IDENTIFICATION, ACTIVATION, SEQUENCE, PROTEIN",

author = "Campbell, {S C} and H Cragg and Elrick, {L J} and Macfarlane, {W M} and Shennan, {K I J} and K Docherty",

year = "1999",

language = "English",

volume = "463",

pages = "53--57",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

}

TY - JOUR

T1 - Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters

AU - Campbell, S C

AU - Cragg, H

AU - Elrick, L J

AU - Macfarlane, W M

AU - Shennan, K I J

AU - Docherty, K

PY - 1999

Y1 - 1999

N2 - Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic beta-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4, The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH-terminus, Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at -253 to -244, and the islet amyloid poly peptide promoter through a sequence element located downstream of -138, The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2-230 and 231-349. (C) 1999 Federation of European Biochemical Societies.

AB - Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic beta-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4, The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH-terminus, Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at -253 to -244, and the islet amyloid poly peptide promoter through a sequence element located downstream of -138, The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2-230 and 231-349. (C) 1999 Federation of European Biochemical Societies.

KW - insulin gene

KW - diabetes mellitus

KW - amylin

KW - transcription

KW - BETA-CELL LINE

KW - MOLECULAR-CLONING

KW - MAMMALIAN-CELLS

KW - PAIRED DOMAIN

KW - GENE

KW - TRANSCRIPTION

KW - IDENTIFICATION

KW - ACTIVATION

KW - SEQUENCE

KW - PROTEIN

M3 - Article

VL - 463

SP - 53

EP - 57

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

ER -

Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters

Abstract

Keywords

UN SDGs

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