Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters

S C Campbell, H Cragg, L J Elrick, W M Macfarlane, K I J Shennan, K Docherty

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic beta-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4, The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH-terminus, Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at -253 to -244, and the islet amyloid poly peptide promoter through a sequence element located downstream of -138, The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2-230 and 231-349. (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)53-57
Number of pages5
JournalFEBS Letters
Volume463
Publication statusPublished - 1999

Keywords

  • insulin gene
  • diabetes mellitus
  • amylin
  • transcription
  • BETA-CELL LINE
  • MOLECULAR-CLONING
  • MAMMALIAN-CELLS
  • PAIRED DOMAIN
  • GENE
  • TRANSCRIPTION
  • IDENTIFICATION
  • ACTIVATION
  • SEQUENCE
  • PROTEIN

Cite this

Campbell, S. C., Cragg, H., Elrick, L. J., Macfarlane, W. M., Shennan, K. I. J., & Docherty, K. (1999). Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters. FEBS Letters, 463, 53-57.

Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters. / Campbell, S C ; Cragg, H ; Elrick, L J ; Macfarlane, W M ; Shennan, K I J ; Docherty, K .

In: FEBS Letters, Vol. 463, 1999, p. 53-57.

Research output: Contribution to journalArticle

Campbell, SC, Cragg, H, Elrick, LJ, Macfarlane, WM, Shennan, KIJ & Docherty, K 1999, 'Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters' FEBS Letters, vol. 463, pp. 53-57.
Campbell SC, Cragg H, Elrick LJ, Macfarlane WM, Shennan KIJ, Docherty K. Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters. FEBS Letters. 1999;463:53-57.
Campbell, S C ; Cragg, H ; Elrick, L J ; Macfarlane, W M ; Shennan, K I J ; Docherty, K . / Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters. In: FEBS Letters. 1999 ; Vol. 463. pp. 53-57.
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AU - Cragg, H

AU - Elrick, L J

AU - Macfarlane, W M

AU - Shennan, K I J

AU - Docherty, K

PY - 1999

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AB - Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic beta-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4, The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH-terminus, Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at -253 to -244, and the islet amyloid poly peptide promoter through a sequence element located downstream of -138, The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2-230 and 231-349. (C) 1999 Federation of European Biochemical Societies.

KW - insulin gene

KW - diabetes mellitus

KW - amylin

KW - transcription

KW - BETA-CELL LINE

KW - MOLECULAR-CLONING

KW - MAMMALIAN-CELLS

KW - PAIRED DOMAIN

KW - GENE

KW - TRANSCRIPTION

KW - IDENTIFICATION

KW - ACTIVATION

KW - SEQUENCE

KW - PROTEIN

M3 - Article

VL - 463

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EP - 57

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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