Inhibitory effect of quercetin on rat trachea contractility in vitro

Raffaele Capasso, Gabriella Aviello, Barbara Romano, Giuseppina Atorino, Ester Pagano, Francesca Borrelli

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

OBJECTIVES: The effect of quercetin, a naturally occurring flavonoid traditionally used to treat airway diseases such as bronchial asthma, on the contractile response elicited by electrical field stimulation or carbachol in rat isolated trachea was investigated.

METHODS: Isolated tracheal tissue was subjected to contractions by an electrical field stimulation of 5 Hz for 30 s, 400 mA, and the responses in the presence of cumulative concentrations of quercetin (10(-6)-3x10(-4) M) were observed. The effect of quercetin was also evaluated after administration of phentolamine plus propranolol (to block alpha- and beta-adrenergic receptors), NG-nitro-L-arginine methyl ester (to block nitric oxide synthesis), capsaicin (to desensitise sensory C fibres), alpha-chymotrypsin (a proteolytic enzyme that rapidly degrades vasoactive intestinal peptide), SR140333 and SR48968 (tackykinin NK1 and NK2 receptor antagonists, respectively).

KEY FINDINGS: Quercetin produced a concentration-dependent inhibition of contractions induced by both carbachol and electrical field stimulation. However, quercetin was more active in inhibiting the contractions produced by electrical field stimulation than those induced by carbachol, suggesting a presynaptic site of action (in addition to a postsynaptic effect, as revealed by the inhibitory action of quercetin on carbachol-induced contractions). The inhibitory effect of quercetin on contractions induced by electrical field stimulation was unaffected by phentolamine plus propranolol, SR 140333 and SR 48968, capsaicin treatment or by the proteolytic enzyme alpha-chymotrypsin. In contrast, the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester significantly reduced the inhibitory effect of quercetin on contractions induced by electrical field stimulation.

CONCLUSIONS: Quercetin inhibits rat tracheal contractility through a presynaptic (involving nitric oxide) and a postsynaptic site of action.

Original languageEnglish
Pages (from-to)115-9
Number of pages5
JournalJournal of Pharmacy and Pharmacology
Volume61
Issue number1
DOIs
Publication statusPublished - Jan 2009

Fingerprint

Quercetin
Trachea
Electric Stimulation
Carbachol
Phentolamine
Capsaicin
NG-Nitroarginine Methyl Ester
Propranolol
Nitric Oxide
Peptide Hydrolases
In Vitro Techniques
Receptors, Adrenergic, alpha
Unmyelinated Nerve Fibers
Vasoactive Intestinal Peptide
Receptors, Adrenergic, beta
Flavonoids
Nitric Oxide Synthase
Asthma

Keywords

  • Adrenergic alpha-Antagonists
  • Adrenergic beta-Antagonists
  • Animals
  • Antioxidants
  • Benzamides
  • Capsaicin
  • Carbachol
  • Dose-Response Relationship, Drug
  • Electric Stimulation
  • Enzyme Inhibitors
  • In Vitro Techniques
  • Male
  • Muscle Contraction
  • Muscle, Smooth
  • NG-Nitroarginine Methyl Ester
  • Neurokinin-1 Receptor Antagonists
  • Nitric Oxide Synthase
  • Phentolamine
  • Piperidines
  • Propranolol
  • Quercetin
  • Quinuclidines
  • Rats
  • Rats, Wistar
  • Receptors, Neurokinin-2
  • Trachea
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Capasso, R., Aviello, G., Romano, B., Atorino, G., Pagano, E., & Borrelli, F. (2009). Inhibitory effect of quercetin on rat trachea contractility in vitro. Journal of Pharmacy and Pharmacology, 61(1), 115-9. https://doi.org/10.1211/jpp/61.01.0016

Inhibitory effect of quercetin on rat trachea contractility in vitro. / Capasso, Raffaele; Aviello, Gabriella; Romano, Barbara; Atorino, Giuseppina; Pagano, Ester; Borrelli, Francesca.

In: Journal of Pharmacy and Pharmacology, Vol. 61, No. 1, 01.2009, p. 115-9.

Research output: Contribution to journalArticle

Capasso, R, Aviello, G, Romano, B, Atorino, G, Pagano, E & Borrelli, F 2009, 'Inhibitory effect of quercetin on rat trachea contractility in vitro' Journal of Pharmacy and Pharmacology, vol. 61, no. 1, pp. 115-9. https://doi.org/10.1211/jpp/61.01.0016
Capasso, Raffaele ; Aviello, Gabriella ; Romano, Barbara ; Atorino, Giuseppina ; Pagano, Ester ; Borrelli, Francesca. / Inhibitory effect of quercetin on rat trachea contractility in vitro. In: Journal of Pharmacy and Pharmacology. 2009 ; Vol. 61, No. 1. pp. 115-9.
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AU - Capasso, Raffaele

AU - Aviello, Gabriella

AU - Romano, Barbara

AU - Atorino, Giuseppina

AU - Pagano, Ester

AU - Borrelli, Francesca

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N2 - OBJECTIVES: The effect of quercetin, a naturally occurring flavonoid traditionally used to treat airway diseases such as bronchial asthma, on the contractile response elicited by electrical field stimulation or carbachol in rat isolated trachea was investigated.METHODS: Isolated tracheal tissue was subjected to contractions by an electrical field stimulation of 5 Hz for 30 s, 400 mA, and the responses in the presence of cumulative concentrations of quercetin (10(-6)-3x10(-4) M) were observed. The effect of quercetin was also evaluated after administration of phentolamine plus propranolol (to block alpha- and beta-adrenergic receptors), NG-nitro-L-arginine methyl ester (to block nitric oxide synthesis), capsaicin (to desensitise sensory C fibres), alpha-chymotrypsin (a proteolytic enzyme that rapidly degrades vasoactive intestinal peptide), SR140333 and SR48968 (tackykinin NK1 and NK2 receptor antagonists, respectively).KEY FINDINGS: Quercetin produced a concentration-dependent inhibition of contractions induced by both carbachol and electrical field stimulation. However, quercetin was more active in inhibiting the contractions produced by electrical field stimulation than those induced by carbachol, suggesting a presynaptic site of action (in addition to a postsynaptic effect, as revealed by the inhibitory action of quercetin on carbachol-induced contractions). The inhibitory effect of quercetin on contractions induced by electrical field stimulation was unaffected by phentolamine plus propranolol, SR 140333 and SR 48968, capsaicin treatment or by the proteolytic enzyme alpha-chymotrypsin. In contrast, the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester significantly reduced the inhibitory effect of quercetin on contractions induced by electrical field stimulation.CONCLUSIONS: Quercetin inhibits rat tracheal contractility through a presynaptic (involving nitric oxide) and a postsynaptic site of action.

AB - OBJECTIVES: The effect of quercetin, a naturally occurring flavonoid traditionally used to treat airway diseases such as bronchial asthma, on the contractile response elicited by electrical field stimulation or carbachol in rat isolated trachea was investigated.METHODS: Isolated tracheal tissue was subjected to contractions by an electrical field stimulation of 5 Hz for 30 s, 400 mA, and the responses in the presence of cumulative concentrations of quercetin (10(-6)-3x10(-4) M) were observed. The effect of quercetin was also evaluated after administration of phentolamine plus propranolol (to block alpha- and beta-adrenergic receptors), NG-nitro-L-arginine methyl ester (to block nitric oxide synthesis), capsaicin (to desensitise sensory C fibres), alpha-chymotrypsin (a proteolytic enzyme that rapidly degrades vasoactive intestinal peptide), SR140333 and SR48968 (tackykinin NK1 and NK2 receptor antagonists, respectively).KEY FINDINGS: Quercetin produced a concentration-dependent inhibition of contractions induced by both carbachol and electrical field stimulation. However, quercetin was more active in inhibiting the contractions produced by electrical field stimulation than those induced by carbachol, suggesting a presynaptic site of action (in addition to a postsynaptic effect, as revealed by the inhibitory action of quercetin on carbachol-induced contractions). The inhibitory effect of quercetin on contractions induced by electrical field stimulation was unaffected by phentolamine plus propranolol, SR 140333 and SR 48968, capsaicin treatment or by the proteolytic enzyme alpha-chymotrypsin. In contrast, the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester significantly reduced the inhibitory effect of quercetin on contractions induced by electrical field stimulation.CONCLUSIONS: Quercetin inhibits rat tracheal contractility through a presynaptic (involving nitric oxide) and a postsynaptic site of action.

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KW - Enzyme Inhibitors

KW - In Vitro Techniques

KW - Male

KW - Muscle Contraction

KW - Muscle, Smooth

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KW - Neurokinin-1 Receptor Antagonists

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KW - Phentolamine

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KW - Propranolol

KW - Quercetin

KW - Quinuclidines

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KW - Rats, Wistar

KW - Receptors, Neurokinin-2

KW - Trachea

KW - Journal Article

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DO - 10.1211/jpp/61.01.0016

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