Insights into piezophily from genetic studies on the deep-sea bacterium, Photobacterium profundum SS9

Ziad W El-Hajj, David Allcock, Theodora Tryfona, Federico M Lauro, Lindsay Sawyer, Douglas H Bartlett, Gail Patricia Ferguson

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The deep-sea bacterium, Photobacterium profundum SS9, has been adopted as a model organism to understand the molecular basis of cold-adapted high-pressure-loving (piezophilic) growth. Despite growing optimally at 28 MPa (15 degrees C), P. profundum SS9 can grow over a wide range of pressures and temperatures. The ability to grow at atmospheric pressure has enabled a limited set of genetic tools to be developed, which has provided genetic insights into the mechanism of piezophilic growth in P. profundum SS9. This review focuses on how genetic studies have uncovered the importance of processes affecting the DNA and the bacterial cell envelope in the piezophilic growth of P. profundum SS9. In addition, a method was developed to assess quantitative piezophilic colony growth of P. profundum SS9 on solid agar. Future studies, using this methodology, could provide novel insights into the molecular basis of piezophilic, surface-attached growth.
Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalAnnals of the New York Academy of Sciences
Volume1189
Issue number1
Early online date1 Mar 2010
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Photobacterium
Oceans and Seas
Bacteria
Growth
Pressure
Bacterial DNA
Atmospheric Pressure
Atmospheric pressure
Agar
Temperature

Keywords

  • Adaptation, Physiological
  • Bacterial Proteins
  • Cell Membrane
  • Cold Temperature
  • DNA, Bacterial
  • Genes, Bacterial
  • Hydrostatic Pressure
  • Models, Biological
  • Photobacterium
  • Seawater
  • cell envelope
  • DNA replication
  • RecD
  • H-NS
  • piezophily

Cite this

El-Hajj, Z. W., Allcock, D., Tryfona, T., Lauro, F. M., Sawyer, L., Bartlett, D. H., & Ferguson, G. P. (2010). Insights into piezophily from genetic studies on the deep-sea bacterium, Photobacterium profundum SS9. Annals of the New York Academy of Sciences, 1189(1), 143-148. https://doi.org/10.1111/j.1749-6632.2009.05178.x

Insights into piezophily from genetic studies on the deep-sea bacterium, Photobacterium profundum SS9. / El-Hajj, Ziad W; Allcock, David; Tryfona, Theodora; Lauro, Federico M; Sawyer, Lindsay; Bartlett, Douglas H; Ferguson, Gail Patricia.

In: Annals of the New York Academy of Sciences, Vol. 1189, No. 1, 03.2010, p. 143-148.

Research output: Contribution to journalArticle

El-Hajj, ZW, Allcock, D, Tryfona, T, Lauro, FM, Sawyer, L, Bartlett, DH & Ferguson, GP 2010, 'Insights into piezophily from genetic studies on the deep-sea bacterium, Photobacterium profundum SS9', Annals of the New York Academy of Sciences, vol. 1189, no. 1, pp. 143-148. https://doi.org/10.1111/j.1749-6632.2009.05178.x
El-Hajj, Ziad W ; Allcock, David ; Tryfona, Theodora ; Lauro, Federico M ; Sawyer, Lindsay ; Bartlett, Douglas H ; Ferguson, Gail Patricia. / Insights into piezophily from genetic studies on the deep-sea bacterium, Photobacterium profundum SS9. In: Annals of the New York Academy of Sciences. 2010 ; Vol. 1189, No. 1. pp. 143-148.
@article{65ce3e7da5544e84b47d48d960e7fcc4,
title = "Insights into piezophily from genetic studies on the deep-sea bacterium, Photobacterium profundum SS9",
abstract = "The deep-sea bacterium, Photobacterium profundum SS9, has been adopted as a model organism to understand the molecular basis of cold-adapted high-pressure-loving (piezophilic) growth. Despite growing optimally at 28 MPa (15 degrees C), P. profundum SS9 can grow over a wide range of pressures and temperatures. The ability to grow at atmospheric pressure has enabled a limited set of genetic tools to be developed, which has provided genetic insights into the mechanism of piezophilic growth in P. profundum SS9. This review focuses on how genetic studies have uncovered the importance of processes affecting the DNA and the bacterial cell envelope in the piezophilic growth of P. profundum SS9. In addition, a method was developed to assess quantitative piezophilic colony growth of P. profundum SS9 on solid agar. Future studies, using this methodology, could provide novel insights into the molecular basis of piezophilic, surface-attached growth.",
keywords = "Adaptation, Physiological, Bacterial Proteins, Cell Membrane, Cold Temperature, DNA, Bacterial, Genes, Bacterial, Hydrostatic Pressure, Models, Biological, Photobacterium, Seawater, cell envelope, DNA replication, RecD, H-NS, piezophily",
author = "El-Hajj, {Ziad W} and David Allcock and Theodora Tryfona and Lauro, {Federico M} and Lindsay Sawyer and Bartlett, {Douglas H} and Ferguson, {Gail Patricia}",
year = "2010",
month = "3",
doi = "10.1111/j.1749-6632.2009.05178.x",
language = "English",
volume = "1189",
pages = "143--148",
journal = "Annals of the New York Academy of Sciences",
issn = "0077-8923",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Insights into piezophily from genetic studies on the deep-sea bacterium, Photobacterium profundum SS9

AU - El-Hajj, Ziad W

AU - Allcock, David

AU - Tryfona, Theodora

AU - Lauro, Federico M

AU - Sawyer, Lindsay

AU - Bartlett, Douglas H

AU - Ferguson, Gail Patricia

PY - 2010/3

Y1 - 2010/3

N2 - The deep-sea bacterium, Photobacterium profundum SS9, has been adopted as a model organism to understand the molecular basis of cold-adapted high-pressure-loving (piezophilic) growth. Despite growing optimally at 28 MPa (15 degrees C), P. profundum SS9 can grow over a wide range of pressures and temperatures. The ability to grow at atmospheric pressure has enabled a limited set of genetic tools to be developed, which has provided genetic insights into the mechanism of piezophilic growth in P. profundum SS9. This review focuses on how genetic studies have uncovered the importance of processes affecting the DNA and the bacterial cell envelope in the piezophilic growth of P. profundum SS9. In addition, a method was developed to assess quantitative piezophilic colony growth of P. profundum SS9 on solid agar. Future studies, using this methodology, could provide novel insights into the molecular basis of piezophilic, surface-attached growth.

AB - The deep-sea bacterium, Photobacterium profundum SS9, has been adopted as a model organism to understand the molecular basis of cold-adapted high-pressure-loving (piezophilic) growth. Despite growing optimally at 28 MPa (15 degrees C), P. profundum SS9 can grow over a wide range of pressures and temperatures. The ability to grow at atmospheric pressure has enabled a limited set of genetic tools to be developed, which has provided genetic insights into the mechanism of piezophilic growth in P. profundum SS9. This review focuses on how genetic studies have uncovered the importance of processes affecting the DNA and the bacterial cell envelope in the piezophilic growth of P. profundum SS9. In addition, a method was developed to assess quantitative piezophilic colony growth of P. profundum SS9 on solid agar. Future studies, using this methodology, could provide novel insights into the molecular basis of piezophilic, surface-attached growth.

KW - Adaptation, Physiological

KW - Bacterial Proteins

KW - Cell Membrane

KW - Cold Temperature

KW - DNA, Bacterial

KW - Genes, Bacterial

KW - Hydrostatic Pressure

KW - Models, Biological

KW - Photobacterium

KW - Seawater

KW - cell envelope

KW - DNA replication

KW - RecD

KW - H-NS

KW - piezophily

U2 - 10.1111/j.1749-6632.2009.05178.x

DO - 10.1111/j.1749-6632.2009.05178.x

M3 - Article

C2 - 20233381

VL - 1189

SP - 143

EP - 148

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

SN - 0077-8923

IS - 1

ER -