Intake compensates for resting metabolic rate variation in female C57BL/6J mice fed high-fat diets

Sarah Louise Johnston, Donna Souter, Bert J. Tolkamp, Iain J. Gordon, Andrew W. Illius, Ilias Kyriazakis, John R. Speakman

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Objective: The literature is divided over whether variation in resting metabolic rate (RMR) is related to subsequent obesity. We set out to see whether the effect of RMR on weight gain in mice could be revealed with high-fat feeding.

Research Methods and Procedures: Female C57BL/6J mice received a low- (10 kcal%fat n = 47), medium- (45 kcal%fat n = 50), or high-fat diet (60 kcal%fat n = 50) for 12 weeks. Pre-treatment RMR was measured by indirect calorimetry. Body composition was estimated using DXA before and after treatment.

Results: Mice on the high-fat diet gained 39% of body mass, whereas control animals gained 3.5%. There was no interaction between RMR and dietary type on weight gain, and there was no association between weight gain and RMR for any of the treatments. RMR accounted for 2.4% of the variation in pre-treatment food intake corrected for initial body mass; however, the gradient of this relationship indicated that variations in RMR were, on average, compensated for by adjustments in food intake.

Discussion: Individual variations in RMR did not predispose mice to weight gain independent of the dietary treatment. Deviations from the relationship between RMR and food intake were not associated with weight gain. This suggests that variations in energy expenditure, caused by RMR and physical activity, are closely linked to dietary intake, and, therefore, well compensated. Individual variations in the strength of this association may underpin individual variability in the responses to diet.

Original languageEnglish
Pages (from-to)600-606
Number of pages7
JournalObesity
Volume15
Issue number3
DOIs
Publication statusPublished - Mar 2007

Keywords

  • high-fat diet
  • fat mass
  • DXA
  • mouse models
  • body-weight gain
  • energy-expenditure
  • induced obesity
  • premenopausal women
  • aerobic fitness
  • white-children
  • Pima-Indians
  • risk factor
  • young-rats
  • leptin

Cite this

Johnston, S. L., Souter, D., Tolkamp, B. J., Gordon, I. J., Illius, A. W., Kyriazakis, I., & Speakman, J. R. (2007). Intake compensates for resting metabolic rate variation in female C57BL/6J mice fed high-fat diets. Obesity, 15(3), 600-606. https://doi.org/10.1038/oby.2007.550

Intake compensates for resting metabolic rate variation in female C57BL/6J mice fed high-fat diets. / Johnston, Sarah Louise; Souter, Donna; Tolkamp, Bert J.; Gordon, Iain J.; Illius, Andrew W.; Kyriazakis, Ilias; Speakman, John R.

In: Obesity, Vol. 15, No. 3, 03.2007, p. 600-606.

Research output: Contribution to journalArticle

Johnston, SL, Souter, D, Tolkamp, BJ, Gordon, IJ, Illius, AW, Kyriazakis, I & Speakman, JR 2007, 'Intake compensates for resting metabolic rate variation in female C57BL/6J mice fed high-fat diets', Obesity, vol. 15, no. 3, pp. 600-606. https://doi.org/10.1038/oby.2007.550
Johnston SL, Souter D, Tolkamp BJ, Gordon IJ, Illius AW, Kyriazakis I et al. Intake compensates for resting metabolic rate variation in female C57BL/6J mice fed high-fat diets. Obesity. 2007 Mar;15(3):600-606. https://doi.org/10.1038/oby.2007.550
Johnston, Sarah Louise ; Souter, Donna ; Tolkamp, Bert J. ; Gordon, Iain J. ; Illius, Andrew W. ; Kyriazakis, Ilias ; Speakman, John R. / Intake compensates for resting metabolic rate variation in female C57BL/6J mice fed high-fat diets. In: Obesity. 2007 ; Vol. 15, No. 3. pp. 600-606.
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AU - Johnston, Sarah Louise

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AU - Kyriazakis, Ilias

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N2 - Objective: The literature is divided over whether variation in resting metabolic rate (RMR) is related to subsequent obesity. We set out to see whether the effect of RMR on weight gain in mice could be revealed with high-fat feeding.Research Methods and Procedures: Female C57BL/6J mice received a low- (10 kcal%fat n = 47), medium- (45 kcal%fat n = 50), or high-fat diet (60 kcal%fat n = 50) for 12 weeks. Pre-treatment RMR was measured by indirect calorimetry. Body composition was estimated using DXA before and after treatment.Results: Mice on the high-fat diet gained 39% of body mass, whereas control animals gained 3.5%. There was no interaction between RMR and dietary type on weight gain, and there was no association between weight gain and RMR for any of the treatments. RMR accounted for 2.4% of the variation in pre-treatment food intake corrected for initial body mass; however, the gradient of this relationship indicated that variations in RMR were, on average, compensated for by adjustments in food intake.Discussion: Individual variations in RMR did not predispose mice to weight gain independent of the dietary treatment. Deviations from the relationship between RMR and food intake were not associated with weight gain. This suggests that variations in energy expenditure, caused by RMR and physical activity, are closely linked to dietary intake, and, therefore, well compensated. Individual variations in the strength of this association may underpin individual variability in the responses to diet.

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KW - white-children

KW - Pima-Indians

KW - risk factor

KW - young-rats

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