Integration site for Streptomyces phage fBT1 and the development of novel site-specific integrating vectors

M. A. Gregory, R. Till, Margaret Caroline MacHin Smith

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184 Citations (Scopus)

Abstract

Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on phiBT1 integrase were shown to have a broad host range and are fully compatible with those based on the phiC31 attP-int locus.

Original languageEnglish
Pages (from-to)5320-5323
Number of pages3
JournalJournal of Bacteriology
Volume185
DOIs
Publication statusPublished - 2003

Keywords

  • ESCHERICHIA-COLI
  • COELICOLOR A3(2)
  • SEQUENCE

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