Integration site for Streptomyces phage fBT1 and the development of novel site-specific integrating vectors

M. A. Gregory, R. Till, Margaret Caroline MacHin Smith

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on phiBT1 integrase were shown to have a broad host range and are fully compatible with those based on the phiC31 attP-int locus.

Original languageEnglish
Pages (from-to)5320-5323
Number of pages3
JournalJournal of Bacteriology
Volume185
DOIs
Publication statusPublished - 2003

Keywords

  • ESCHERICHIA-COLI
  • COELICOLOR A3(2)
  • SEQUENCE

Cite this

Integration site for Streptomyces phage fBT1 and the development of novel site-specific integrating vectors. / Gregory, M. A.; Till, R.; Smith, Margaret Caroline MacHin.

In: Journal of Bacteriology, Vol. 185, 2003, p. 5320-5323.

Research output: Contribution to journalArticle

Gregory, M. A. ; Till, R. ; Smith, Margaret Caroline MacHin. / Integration site for Streptomyces phage fBT1 and the development of novel site-specific integrating vectors. In: Journal of Bacteriology. 2003 ; Vol. 185. pp. 5320-5323.
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