Inter-individual variability in the production of flavan-3-ol colonic metabolites: preliminary elucidation of urinary metabotypes

Pedro Mena; Iziar A Ludwig; Virginia B Tomatis; Animesh Acharjee; Luca Calani; Alice Rosi; Furio Brighenti; Sumantra Ray; Julian L Griffin; Les J Bluck; Daniele Del Rio

doi:10.1007/s00394-018-1683-4

Inter-individual variability in the production of flavan-3-ol colonic metabolites: preliminary elucidation of urinary metabotypes

Pedro Mena^* (Corresponding Author), Iziar A Ludwig, Virginia B Tomatis, Animesh Acharjee, Luca Calani, Alice Rosi, Furio Brighenti, Sumantra Ray, Julian L Griffin, Les J Bluck, Daniele Del Rio^* (Corresponding Author)

^*Corresponding author for this work

The Rowett Institute of Nutrition and Health

University of Parma

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

PURPOSE: There is much information on the bioavailability of (poly)phenolic compounds following acute intake of various foods. However, there are only limited data on the effects of repeated and combined exposure to specific (poly)phenol food sources and the inter-individual variability in their bioavailability. This study evaluated the combined urinary excretion of (poly)phenols from green tea and coffee following daily consumption by healthy subjects in free-living conditions. The inter-individual variability in the production of phenolic metabolites was also investigated.

METHODS: Eleven participants consumed both tablets of green tea and green coffee bean extracts daily for 8 weeks and 24-h urine was collected on five different occasions. The urinary profile of phenolic metabolites and a set of multivariate statistical tests were used to investigate the putative existence of characteristic metabotypes in the production of flavan-3-ol microbial metabolites.

RESULTS: (Poly)phenolic compounds in the green tea and green coffee bean extracts were absorbed and excreted after simultaneous consumption, with green tea resulting in more inter-individual variability in urinary excretion of phenolic metabolites. Three metabotypes in the production of flavan-3-ol microbial metabolites were tentatively defined, characterized by the excretion of different amounts of trihydroxyphenyl-γ-valerolactones, dihydroxyphenyl-γ-valerolactones, and hydroxyphenylpropionic acids.

CONCLUSIONS: The selective production of microbiota-derived metabolites from flavan-3-ols and the putative existence of characteristic metabotypes in their production represent an important development in the study of the bioavailability of plant bioactives. These observations will contribute to better understand the health effects and individual differences associated with consumption of flavan-3-ols, arguably the main class of flavonoids in the human diet.

Original language	English
Pages (from-to)	1529-1543
Number of pages	15
Journal	European Journal of Nutrition
Volume	58
Issue number	4
Early online date	3 Apr 2018
DOIs	https://doi.org/10.1007/s00394-018-1683-4
Publication status	Published - Jun 2019

Bibliographical note

This work was partially funded by MRC core funding (Physi-
ological Modelling of Metabolic Risk, MC_UP_A090_1005, and
Nutrition, Surveys and Studies, MC_U105960384) and University of
Parma core funding (FIL 2014-2017). P.M. was partially funded by a
Grant of the Postdoctoral Fellowship Program from Fundación Séneca
(Murcia Region, Spain). I.A.L. was supported by a postdoctoral fellow-
ship funded by the Spanish Ministry of Economy and Competitiveness
(IJCI-2014-20689).

Data Availability Statement

No data availability statement.

Keywords

Adolescent
Adult
Biological Availability
Coffee/metabolism
Colon/metabolism
Female
Flavonoids/urine
Humans
Male
Middle Aged
Polyphenols/urine
Tea/metabolism
Young Adult

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00394-018-1683-4

Cite this

Mena, P., Ludwig, I. A., Tomatis, V. B., Acharjee, A., Calani, L., Rosi, A., Brighenti, F., Ray, S., Griffin, J. L., Bluck, L. J., & Del Rio, D. (2019). Inter-individual variability in the production of flavan-3-ol colonic metabolites: preliminary elucidation of urinary metabotypes. European Journal of Nutrition, 58(4), 1529-1543. https://doi.org/10.1007/s00394-018-1683-4

@article{a0d8ecc7e3914e409a4280b7219800c4,

title = "Inter-individual variability in the production of flavan-3-ol colonic metabolites: preliminary elucidation of urinary metabotypes",

abstract = "PURPOSE: There is much information on the bioavailability of (poly)phenolic compounds following acute intake of various foods. However, there are only limited data on the effects of repeated and combined exposure to specific (poly)phenol food sources and the inter-individual variability in their bioavailability. This study evaluated the combined urinary excretion of (poly)phenols from green tea and coffee following daily consumption by healthy subjects in free-living conditions. The inter-individual variability in the production of phenolic metabolites was also investigated.METHODS: Eleven participants consumed both tablets of green tea and green coffee bean extracts daily for 8 weeks and 24-h urine was collected on five different occasions. The urinary profile of phenolic metabolites and a set of multivariate statistical tests were used to investigate the putative existence of characteristic metabotypes in the production of flavan-3-ol microbial metabolites.RESULTS: (Poly)phenolic compounds in the green tea and green coffee bean extracts were absorbed and excreted after simultaneous consumption, with green tea resulting in more inter-individual variability in urinary excretion of phenolic metabolites. Three metabotypes in the production of flavan-3-ol microbial metabolites were tentatively defined, characterized by the excretion of different amounts of trihydroxyphenyl-γ-valerolactones, dihydroxyphenyl-γ-valerolactones, and hydroxyphenylpropionic acids.CONCLUSIONS: The selective production of microbiota-derived metabolites from flavan-3-ols and the putative existence of characteristic metabotypes in their production represent an important development in the study of the bioavailability of plant bioactives. These observations will contribute to better understand the health effects and individual differences associated with consumption of flavan-3-ols, arguably the main class of flavonoids in the human diet.",

keywords = "Adolescent, Adult, Biological Availability, Coffee/metabolism, Colon/metabolism, Female, Flavonoids/urine, Humans, Male, Middle Aged, Polyphenols/urine, Tea/metabolism, Young Adult",

author = "Pedro Mena and Ludwig, {Iziar A} and Tomatis, {Virginia B} and Animesh Acharjee and Luca Calani and Alice Rosi and Furio Brighenti and Sumantra Ray and Griffin, {Julian L} and Bluck, {Les J} and {Del Rio}, Daniele",

note = "This work was partially funded by MRC core funding (Physi- ological Modelling of Metabolic Risk, MC_UP_A090_1005, and Nutrition, Surveys and Studies, MC_U105960384) and University of Parma core funding (FIL 2014-2017). P.M. was partially funded by a Grant of the Postdoctoral Fellowship Program from Fundaci{\'o}n S{\'e}neca (Murcia Region, Spain). I.A.L. was supported by a postdoctoral fellow- ship funded by the Spanish Ministry of Economy and Competitiveness (IJCI-2014-20689).",

year = "2019",

month = jun,

doi = "10.1007/s00394-018-1683-4",

language = "English",

volume = "58",

pages = "1529--1543",

journal = "European Journal of Nutrition",

issn = "1436-6207",

publisher = "D. Steinkopff-Verlag",

number = "4",

}

TY - JOUR

T1 - Inter-individual variability in the production of flavan-3-ol colonic metabolites

T2 - preliminary elucidation of urinary metabotypes

AU - Mena, Pedro

AU - Ludwig, Iziar A

AU - Tomatis, Virginia B

AU - Acharjee, Animesh

AU - Calani, Luca

AU - Rosi, Alice

AU - Brighenti, Furio

AU - Ray, Sumantra

AU - Griffin, Julian L

AU - Bluck, Les J

AU - Del Rio, Daniele

N1 - This work was partially funded by MRC core funding (Physi- ological Modelling of Metabolic Risk, MC_UP_A090_1005, and Nutrition, Surveys and Studies, MC_U105960384) and University of Parma core funding (FIL 2014-2017). P.M. was partially funded by a Grant of the Postdoctoral Fellowship Program from Fundación Séneca (Murcia Region, Spain). I.A.L. was supported by a postdoctoral fellow- ship funded by the Spanish Ministry of Economy and Competitiveness (IJCI-2014-20689).

PY - 2019/6

Y1 - 2019/6

N2 - PURPOSE: There is much information on the bioavailability of (poly)phenolic compounds following acute intake of various foods. However, there are only limited data on the effects of repeated and combined exposure to specific (poly)phenol food sources and the inter-individual variability in their bioavailability. This study evaluated the combined urinary excretion of (poly)phenols from green tea and coffee following daily consumption by healthy subjects in free-living conditions. The inter-individual variability in the production of phenolic metabolites was also investigated.METHODS: Eleven participants consumed both tablets of green tea and green coffee bean extracts daily for 8 weeks and 24-h urine was collected on five different occasions. The urinary profile of phenolic metabolites and a set of multivariate statistical tests were used to investigate the putative existence of characteristic metabotypes in the production of flavan-3-ol microbial metabolites.RESULTS: (Poly)phenolic compounds in the green tea and green coffee bean extracts were absorbed and excreted after simultaneous consumption, with green tea resulting in more inter-individual variability in urinary excretion of phenolic metabolites. Three metabotypes in the production of flavan-3-ol microbial metabolites were tentatively defined, characterized by the excretion of different amounts of trihydroxyphenyl-γ-valerolactones, dihydroxyphenyl-γ-valerolactones, and hydroxyphenylpropionic acids.CONCLUSIONS: The selective production of microbiota-derived metabolites from flavan-3-ols and the putative existence of characteristic metabotypes in their production represent an important development in the study of the bioavailability of plant bioactives. These observations will contribute to better understand the health effects and individual differences associated with consumption of flavan-3-ols, arguably the main class of flavonoids in the human diet.

AB - PURPOSE: There is much information on the bioavailability of (poly)phenolic compounds following acute intake of various foods. However, there are only limited data on the effects of repeated and combined exposure to specific (poly)phenol food sources and the inter-individual variability in their bioavailability. This study evaluated the combined urinary excretion of (poly)phenols from green tea and coffee following daily consumption by healthy subjects in free-living conditions. The inter-individual variability in the production of phenolic metabolites was also investigated.METHODS: Eleven participants consumed both tablets of green tea and green coffee bean extracts daily for 8 weeks and 24-h urine was collected on five different occasions. The urinary profile of phenolic metabolites and a set of multivariate statistical tests were used to investigate the putative existence of characteristic metabotypes in the production of flavan-3-ol microbial metabolites.RESULTS: (Poly)phenolic compounds in the green tea and green coffee bean extracts were absorbed and excreted after simultaneous consumption, with green tea resulting in more inter-individual variability in urinary excretion of phenolic metabolites. Three metabotypes in the production of flavan-3-ol microbial metabolites were tentatively defined, characterized by the excretion of different amounts of trihydroxyphenyl-γ-valerolactones, dihydroxyphenyl-γ-valerolactones, and hydroxyphenylpropionic acids.CONCLUSIONS: The selective production of microbiota-derived metabolites from flavan-3-ols and the putative existence of characteristic metabotypes in their production represent an important development in the study of the bioavailability of plant bioactives. These observations will contribute to better understand the health effects and individual differences associated with consumption of flavan-3-ols, arguably the main class of flavonoids in the human diet.

KW - Adolescent

KW - Adult

KW - Biological Availability

KW - Coffee/metabolism

KW - Colon/metabolism

KW - Female

KW - Flavonoids/urine

KW - Humans

KW - Male

KW - Middle Aged

KW - Polyphenols/urine

KW - Tea/metabolism

KW - Young Adult

U2 - 10.1007/s00394-018-1683-4

DO - 10.1007/s00394-018-1683-4

M3 - Article

C2 - 29616322

SN - 1436-6207

VL - 58

SP - 1529

EP - 1543

JO - European Journal of Nutrition

JF - European Journal of Nutrition

IS - 4

ER -

Inter-individual variability in the production of flavan-3-ol colonic metabolites: preliminary elucidation of urinary metabotypes

Abstract

Bibliographical note

Data Availability Statement

Keywords

UN SDGs

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