Inter-individual variation in postprandial glycaemic responses in women co-ingesting green leafy vegetables with a carbohydrate meal: interactions with the sirtuin system

Marietta Sayegh; Jaye Henderson; Andrew J Farquharson; Graham Horgan; Viren Ranawana; Janice E Drew

doi:10.1002/mnfr.202000923

Inter-individual variation in postprandial glycaemic responses in women co-ingesting green leafy vegetables with a carbohydrate meal: interactions with the sirtuin system

Marietta Sayegh, Jaye Henderson, Andrew J Farquharson, Graham Horgan, Viren Ranawana, Janice E Drew^* (Corresponding Author)

^*Corresponding author for this work

The Rowett Institute of Nutrition and Health

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Abstract

SCOPE: Green leafy vegetables (GLV) may improve postprandial glycaemic responses (PGR) and metabolic health. However, inter-individual variations (IIV) preclude conclusive evidence. Sirtuin system is emerging as a key player in blood glucose control. This study investigated IIV in PGR in women co-ingesting GLV with a carbohydrate meal and interactions with the sirtuin system.

METHODS AND RESULTS: Volunteers (n = 31 women) consumed rice, rice with bok choy or spinach (75g available carbohydrate) on separate occasions. Postprandial glucose, insulin, adropin and lipid levels were measured. Anthropometric measurements and sex hormones were measured. GeXP assay measured whole blood postprandial gene expression profiles of 25 markers involved in sirtuin signalling. GLV consumption had no significant effect on PGR which showed high variation. PGR correlated with age, but no other consistent associations were observed. Sirtuin gene expression profiles revealed distinct stratified subgroups associated with PGR, lipid, insulin, fat mass, waist/hip circumferences and adropin levels.

CONCLUSION: PGR to co-ingesting GLV with a carbohydrate meal were highly variable in this cohort and failed to reveal a significant reduction in PGR. Variable responses were largely independent of menopausal status and meal consumed. However, lower expression of sirtuin gene targets was associated with higher PGR and with markers linked to health status. This article is protected by copyright. All rights reserved.

Original language	English
Article number	e2000923
Number of pages	10
Journal	Molecular Nutrition & Food Research
Volume	65
Issue number	13
Early online date	7 Jun 2021
DOIs	https://doi.org/10.1002/mnfr.202000923
Publication status	Published - 1 Jul 2021

Keywords

carbohydrates
inter‐individual variation
sirtuins
postprandial glycaemia
women

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Inter-individual variation in postprandial glycaemic responses in women co-ingesting green leafy vegetables with a carbohydrate meal : interactions with the sirtuin system. / Sayegh, Marietta; Henderson, Jaye; Farquharson, Andrew J et al.

In: Molecular Nutrition & Food Research, Vol. 65, No. 13, e2000923, 01.07.2021.

Research output: Contribution to journal › Article › peer-review

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title = "Inter-individual variation in postprandial glycaemic responses in women co-ingesting green leafy vegetables with a carbohydrate meal: interactions with the sirtuin system",

abstract = "SCOPE: Green leafy vegetables (GLV) may improve postprandial glycaemic responses (PGR) and metabolic health. However, inter-individual variations (IIV) preclude conclusive evidence. Sirtuin system is emerging as a key player in blood glucose control. This study investigated IIV in PGR in women co-ingesting GLV with a carbohydrate meal and interactions with the sirtuin system.METHODS AND RESULTS: Volunteers (n = 31 women) consumed rice, rice with bok choy or spinach (75g available carbohydrate) on separate occasions. Postprandial glucose, insulin, adropin and lipid levels were measured. Anthropometric measurements and sex hormones were measured. GeXP assay measured whole blood postprandial gene expression profiles of 25 markers involved in sirtuin signalling. GLV consumption had no significant effect on PGR which showed high variation. PGR correlated with age, but no other consistent associations were observed. Sirtuin gene expression profiles revealed distinct stratified subgroups associated with PGR, lipid, insulin, fat mass, waist/hip circumferences and adropin levels.CONCLUSION: PGR to co-ingesting GLV with a carbohydrate meal were highly variable in this cohort and failed to reveal a significant reduction in PGR. Variable responses were largely independent of menopausal status and meal consumed. However, lower expression of sirtuin gene targets was associated with higher PGR and with markers linked to health status. This article is protected by copyright. All rights reserved.",

keywords = "carbohydrates, inter‐individual variation, sirtuins, postprandial glycaemia, women",

author = "Marietta Sayegh and Jaye Henderson and Farquharson, {Andrew J} and Graham Horgan and Viren Ranawana and Drew, {Janice E}",

note = "The authors acknowledge the support of the Scottish Government Rural and Environment Science and Analytical Services (RESAS) Strategic Research Programme. Clinical biochemistry lab at the Foresterhill hospital for analysing sex hormone samples, Human Nutrition Unit staff and analytical staff and the Rowett Institute, University of Aberdeen and Alex Stewart for providing the compositional information for the meal interventions used. Finally, we would like to thank the volunteers participating in VegGI study. Open access via Wiley agreement",

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AU - Sayegh, Marietta

AU - Henderson, Jaye

AU - Farquharson, Andrew J

AU - Horgan, Graham

AU - Ranawana, Viren

AU - Drew, Janice E

N1 - The authors acknowledge the support of the Scottish Government Rural and Environment Science and Analytical Services (RESAS) Strategic Research Programme. Clinical biochemistry lab at the Foresterhill hospital for analysing sex hormone samples, Human Nutrition Unit staff and analytical staff and the Rowett Institute, University of Aberdeen and Alex Stewart for providing the compositional information for the meal interventions used. Finally, we would like to thank the volunteers participating in VegGI study. Open access via Wiley agreement

PY - 2021/7/1

Y1 - 2021/7/1

N2 - SCOPE: Green leafy vegetables (GLV) may improve postprandial glycaemic responses (PGR) and metabolic health. However, inter-individual variations (IIV) preclude conclusive evidence. Sirtuin system is emerging as a key player in blood glucose control. This study investigated IIV in PGR in women co-ingesting GLV with a carbohydrate meal and interactions with the sirtuin system.METHODS AND RESULTS: Volunteers (n = 31 women) consumed rice, rice with bok choy or spinach (75g available carbohydrate) on separate occasions. Postprandial glucose, insulin, adropin and lipid levels were measured. Anthropometric measurements and sex hormones were measured. GeXP assay measured whole blood postprandial gene expression profiles of 25 markers involved in sirtuin signalling. GLV consumption had no significant effect on PGR which showed high variation. PGR correlated with age, but no other consistent associations were observed. Sirtuin gene expression profiles revealed distinct stratified subgroups associated with PGR, lipid, insulin, fat mass, waist/hip circumferences and adropin levels.CONCLUSION: PGR to co-ingesting GLV with a carbohydrate meal were highly variable in this cohort and failed to reveal a significant reduction in PGR. Variable responses were largely independent of menopausal status and meal consumed. However, lower expression of sirtuin gene targets was associated with higher PGR and with markers linked to health status. This article is protected by copyright. All rights reserved.

AB - SCOPE: Green leafy vegetables (GLV) may improve postprandial glycaemic responses (PGR) and metabolic health. However, inter-individual variations (IIV) preclude conclusive evidence. Sirtuin system is emerging as a key player in blood glucose control. This study investigated IIV in PGR in women co-ingesting GLV with a carbohydrate meal and interactions with the sirtuin system.METHODS AND RESULTS: Volunteers (n = 31 women) consumed rice, rice with bok choy or spinach (75g available carbohydrate) on separate occasions. Postprandial glucose, insulin, adropin and lipid levels were measured. Anthropometric measurements and sex hormones were measured. GeXP assay measured whole blood postprandial gene expression profiles of 25 markers involved in sirtuin signalling. GLV consumption had no significant effect on PGR which showed high variation. PGR correlated with age, but no other consistent associations were observed. Sirtuin gene expression profiles revealed distinct stratified subgroups associated with PGR, lipid, insulin, fat mass, waist/hip circumferences and adropin levels.CONCLUSION: PGR to co-ingesting GLV with a carbohydrate meal were highly variable in this cohort and failed to reveal a significant reduction in PGR. Variable responses were largely independent of menopausal status and meal consumed. However, lower expression of sirtuin gene targets was associated with higher PGR and with markers linked to health status. This article is protected by copyright. All rights reserved.

KW - carbohydrates

KW - inter‐individual variation

KW - sirtuins

KW - postprandial glycaemia

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DO - 10.1002/mnfr.202000923

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JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

IS - 13

M1 - e2000923

ER -

Inter-individual variation in postprandial glycaemic responses in women co-ingesting green leafy vegetables with a carbohydrate meal: interactions with the sirtuin system

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