Interactions between N application rate, CH4 oxidation and N2O production in soil

Stuart Acton, Elizabeth Baggs

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Here we report on a controlled environment experiment in which we applied C-13- and N-15-enrichment approaches to quantify methane oxidation rates and source partition N2O production in a silt loam soil following application of NH4NO3, enabling us to look for potential interactions between methane oxidation and nitrifier-N2O production. N-15-N2O, N14+15-N2O and CO2 fluxes and mineral N concentrations were measured over a 23-day period after application of NH4NO3 (5 at.% excess N-15) at rates of 0, 5, 10, 20, 30 and 40 g N m(-2) to a silt loam soil. Change in C-12/13-CH4 concentrations (as indicative of C-13-CH4 oxidation rates) and production of C-13-CO2 were monitored over the first 72 h after addition of 1.7 mu l C-13-CH4 l(-1) (10 at.% excess C-13) to these N treatments. Oxidation of applied C-13-CH4 was slower in the 5, 10, 20 and 30 g N m(-2) (5 at.% excess N-15) treatments (0.24-0.32 mu g C-13-CH4 l(-1) day(-1)) than in the control (0.40 mu g C-13-CH4 l(-1) day(-1)), suggesting that these N loadings inhibited oxidation. N2O production was raised after N addition, and in the 10, 20 and 30 g N m(-2) treatments nitrification was the predominant source of N2O accounting for 61, 83 and 57% of the total N-15-N2O produced, respectively. Our results point towards the possibility of methylotrophs switching function to oxidise ammonia in the presence of N, which may result in greater atmospheric loading of both CH4 and N2O.

Original languageEnglish
Pages (from-to)15-26
Number of pages12
JournalBiogeochemistry
Volume103
Issue number1-3
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Soils
oxidation
Oxidation
Silt
Methane
loam
soil
silt
methane
Switching functions
Nitrification
Ammonia
Minerals
nitrification
ammonia
rate
Fluxes
mineral
experiment
Experiments

Keywords

  • Denitrification
  • Methane oxidation
  • Nitrification
  • Nitrous oxide
  • Soil
  • Stable isotopes
  • Tempertate forest soils
  • Atmospheric methane
  • Nitrous-oxide emissions
  • Short-term
  • Isotope fractionation
  • Agricultural soils
  • Arable soils
  • Carbon
  • Ammonium

Cite this

Interactions between N application rate, CH4 oxidation and N2O production in soil. / Acton, Stuart; Baggs, Elizabeth.

In: Biogeochemistry, Vol. 103, No. 1-3, 04.2011, p. 15-26.

Research output: Contribution to journalArticle

Acton, Stuart ; Baggs, Elizabeth. / Interactions between N application rate, CH4 oxidation and N2O production in soil. In: Biogeochemistry. 2011 ; Vol. 103, No. 1-3. pp. 15-26.
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abstract = "Here we report on a controlled environment experiment in which we applied C-13- and N-15-enrichment approaches to quantify methane oxidation rates and source partition N2O production in a silt loam soil following application of NH4NO3, enabling us to look for potential interactions between methane oxidation and nitrifier-N2O production. N-15-N2O, N14+15-N2O and CO2 fluxes and mineral N concentrations were measured over a 23-day period after application of NH4NO3 (5 at.{\%} excess N-15) at rates of 0, 5, 10, 20, 30 and 40 g N m(-2) to a silt loam soil. Change in C-12/13-CH4 concentrations (as indicative of C-13-CH4 oxidation rates) and production of C-13-CO2 were monitored over the first 72 h after addition of 1.7 mu l C-13-CH4 l(-1) (10 at.{\%} excess C-13) to these N treatments. Oxidation of applied C-13-CH4 was slower in the 5, 10, 20 and 30 g N m(-2) (5 at.{\%} excess N-15) treatments (0.24-0.32 mu g C-13-CH4 l(-1) day(-1)) than in the control (0.40 mu g C-13-CH4 l(-1) day(-1)), suggesting that these N loadings inhibited oxidation. N2O production was raised after N addition, and in the 10, 20 and 30 g N m(-2) treatments nitrification was the predominant source of N2O accounting for 61, 83 and 57{\%} of the total N-15-N2O produced, respectively. Our results point towards the possibility of methylotrophs switching function to oxidise ammonia in the presence of N, which may result in greater atmospheric loading of both CH4 and N2O.",
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T1 - Interactions between N application rate, CH4 oxidation and N2O production in soil

AU - Acton, Stuart

AU - Baggs, Elizabeth

PY - 2011/4

Y1 - 2011/4

N2 - Here we report on a controlled environment experiment in which we applied C-13- and N-15-enrichment approaches to quantify methane oxidation rates and source partition N2O production in a silt loam soil following application of NH4NO3, enabling us to look for potential interactions between methane oxidation and nitrifier-N2O production. N-15-N2O, N14+15-N2O and CO2 fluxes and mineral N concentrations were measured over a 23-day period after application of NH4NO3 (5 at.% excess N-15) at rates of 0, 5, 10, 20, 30 and 40 g N m(-2) to a silt loam soil. Change in C-12/13-CH4 concentrations (as indicative of C-13-CH4 oxidation rates) and production of C-13-CO2 were monitored over the first 72 h after addition of 1.7 mu l C-13-CH4 l(-1) (10 at.% excess C-13) to these N treatments. Oxidation of applied C-13-CH4 was slower in the 5, 10, 20 and 30 g N m(-2) (5 at.% excess N-15) treatments (0.24-0.32 mu g C-13-CH4 l(-1) day(-1)) than in the control (0.40 mu g C-13-CH4 l(-1) day(-1)), suggesting that these N loadings inhibited oxidation. N2O production was raised after N addition, and in the 10, 20 and 30 g N m(-2) treatments nitrification was the predominant source of N2O accounting for 61, 83 and 57% of the total N-15-N2O produced, respectively. Our results point towards the possibility of methylotrophs switching function to oxidise ammonia in the presence of N, which may result in greater atmospheric loading of both CH4 and N2O.

AB - Here we report on a controlled environment experiment in which we applied C-13- and N-15-enrichment approaches to quantify methane oxidation rates and source partition N2O production in a silt loam soil following application of NH4NO3, enabling us to look for potential interactions between methane oxidation and nitrifier-N2O production. N-15-N2O, N14+15-N2O and CO2 fluxes and mineral N concentrations were measured over a 23-day period after application of NH4NO3 (5 at.% excess N-15) at rates of 0, 5, 10, 20, 30 and 40 g N m(-2) to a silt loam soil. Change in C-12/13-CH4 concentrations (as indicative of C-13-CH4 oxidation rates) and production of C-13-CO2 were monitored over the first 72 h after addition of 1.7 mu l C-13-CH4 l(-1) (10 at.% excess C-13) to these N treatments. Oxidation of applied C-13-CH4 was slower in the 5, 10, 20 and 30 g N m(-2) (5 at.% excess N-15) treatments (0.24-0.32 mu g C-13-CH4 l(-1) day(-1)) than in the control (0.40 mu g C-13-CH4 l(-1) day(-1)), suggesting that these N loadings inhibited oxidation. N2O production was raised after N addition, and in the 10, 20 and 30 g N m(-2) treatments nitrification was the predominant source of N2O accounting for 61, 83 and 57% of the total N-15-N2O produced, respectively. Our results point towards the possibility of methylotrophs switching function to oxidise ammonia in the presence of N, which may result in greater atmospheric loading of both CH4 and N2O.

KW - Denitrification

KW - Methane oxidation

KW - Nitrification

KW - Nitrous oxide

KW - Soil

KW - Stable isotopes

KW - Tempertate forest soils

KW - Atmospheric methane

KW - Nitrous-oxide emissions

KW - Short-term

KW - Isotope fractionation

KW - Agricultural soils

KW - Arable soils

KW - Carbon

KW - Ammonium

U2 - 10.1007/s10533-010-9442-5

DO - 10.1007/s10533-010-9442-5

M3 - Article

VL - 103

SP - 15

EP - 26

JO - Biogeochemistry

JF - Biogeochemistry

SN - 0168-2563

IS - 1-3

ER -