Interactions between soil, toxicant, and a lux-marked bacterium during solid phase-contact toxicity testing

L J Shaw, Y Beaton, L A Glover, K Killham, A A Meharg

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Bioluminescence-based, solid-contact toxicity assays allow test bacterium and toxicant to interact at the solid-solution interface. A (lux-marked bacterium, Burkholderia sp. RASC, and 2,4-dichlorophenol (2,4-DCP) were used to characterize these interactions. In the basic bioassay, cells were added to soil slurries containing 2,4-DCP (0-120 mu g ml(-1)). After 15 min, soil was removed by centrifugation, and bioluminescence in the supernatant was determined. Investigation of 2,4-DCP adsorption to soil revealed that sorption was linear and not significantly (p > 0.1) affected by the presence of Burkholderia cells. The numbers of culturable Burkholderia cells in the assay supernatant were 48.2 to 64.8% of the inoculum and independent of the soil weight. The effect of soil on 2,4-DCP toxicity was investigated by comparing soil aqueous extract and contact assays. The percentage bioluminescence for the contact assay was consistently higher than the extract assay at all test concentrations, and counts of viable Burkholderia cells were enhanced by the presence of 2,4-DCP in the contact assay. Expressing results as specific bioluminescence decreased the variability in response and the discrepancy in results between the two protocols. We suggest that solid-contact assays need improvement to ensure defined contact between cells and solid phase, and that the reporting of specific activity should be emphasized.

Original languageEnglish
Pages (from-to)1247-1252
Number of pages6
JournalEnvironmental Toxicology and Chemistry
Volume19
Publication statusPublished - 2000

Keywords

  • soil
  • lux
  • 2,4-dichlorophenol
  • toxicity
  • bioassay
  • SEDIMENT COMPOSITION
  • SORPTION
  • BIOAVAILABILITY
  • PENTACHLOROPHENOL
  • MICROTOX(R)
  • TRANSPORT
  • ACID
  • PH

Cite this

Interactions between soil, toxicant, and a lux-marked bacterium during solid phase-contact toxicity testing. / Shaw, L J ; Beaton, Y ; Glover, L A ; Killham, K ; Meharg, A A .

In: Environmental Toxicology and Chemistry, Vol. 19, 2000, p. 1247-1252.

Research output: Contribution to journalArticle

Shaw, L J ; Beaton, Y ; Glover, L A ; Killham, K ; Meharg, A A . / Interactions between soil, toxicant, and a lux-marked bacterium during solid phase-contact toxicity testing. In: Environmental Toxicology and Chemistry. 2000 ; Vol. 19. pp. 1247-1252.
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T1 - Interactions between soil, toxicant, and a lux-marked bacterium during solid phase-contact toxicity testing

AU - Shaw, L J

AU - Beaton, Y

AU - Glover, L A

AU - Killham, K

AU - Meharg, A A

PY - 2000

Y1 - 2000

N2 - Bioluminescence-based, solid-contact toxicity assays allow test bacterium and toxicant to interact at the solid-solution interface. A (lux-marked bacterium, Burkholderia sp. RASC, and 2,4-dichlorophenol (2,4-DCP) were used to characterize these interactions. In the basic bioassay, cells were added to soil slurries containing 2,4-DCP (0-120 mu g ml(-1)). After 15 min, soil was removed by centrifugation, and bioluminescence in the supernatant was determined. Investigation of 2,4-DCP adsorption to soil revealed that sorption was linear and not significantly (p > 0.1) affected by the presence of Burkholderia cells. The numbers of culturable Burkholderia cells in the assay supernatant were 48.2 to 64.8% of the inoculum and independent of the soil weight. The effect of soil on 2,4-DCP toxicity was investigated by comparing soil aqueous extract and contact assays. The percentage bioluminescence for the contact assay was consistently higher than the extract assay at all test concentrations, and counts of viable Burkholderia cells were enhanced by the presence of 2,4-DCP in the contact assay. Expressing results as specific bioluminescence decreased the variability in response and the discrepancy in results between the two protocols. We suggest that solid-contact assays need improvement to ensure defined contact between cells and solid phase, and that the reporting of specific activity should be emphasized.

AB - Bioluminescence-based, solid-contact toxicity assays allow test bacterium and toxicant to interact at the solid-solution interface. A (lux-marked bacterium, Burkholderia sp. RASC, and 2,4-dichlorophenol (2,4-DCP) were used to characterize these interactions. In the basic bioassay, cells were added to soil slurries containing 2,4-DCP (0-120 mu g ml(-1)). After 15 min, soil was removed by centrifugation, and bioluminescence in the supernatant was determined. Investigation of 2,4-DCP adsorption to soil revealed that sorption was linear and not significantly (p > 0.1) affected by the presence of Burkholderia cells. The numbers of culturable Burkholderia cells in the assay supernatant were 48.2 to 64.8% of the inoculum and independent of the soil weight. The effect of soil on 2,4-DCP toxicity was investigated by comparing soil aqueous extract and contact assays. The percentage bioluminescence for the contact assay was consistently higher than the extract assay at all test concentrations, and counts of viable Burkholderia cells were enhanced by the presence of 2,4-DCP in the contact assay. Expressing results as specific bioluminescence decreased the variability in response and the discrepancy in results between the two protocols. We suggest that solid-contact assays need improvement to ensure defined contact between cells and solid phase, and that the reporting of specific activity should be emphasized.

KW - soil

KW - lux

KW - 2,4-dichlorophenol

KW - toxicity

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KW - SORPTION

KW - BIOAVAILABILITY

KW - PENTACHLOROPHENOL

KW - MICROTOX(R)

KW - TRANSPORT

KW - ACID

KW - PH

M3 - Article

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SP - 1247

EP - 1252

JO - Environmental Toxicology and Chemistry

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SN - 0730-7268

ER -