Intercellular Interferons in Fish

A unique means to combat viral infection

Chang Ming Xian, Jun Zou, Pin Nie, Bei Huang, Zhanglong Yu, Bertrand Collet, Christopher John Secombes

Research output: Non-textual formDatabase

Abstract

We demonstrate for the first time in vertebrates, that alternative splicing of interferon (IFN) genes can lead to a functional intracellular IFN (iIFN). Fish IFN genes possess introns and in rainbow trout three alternatively spliced transcripts of the IFN1 gene exist. Two of the encoded IFNs are predicted to lack a signal peptide. When overexpressed these iIFNs induce antiviral responses. Variants of the two IFNR receptor chains (IFNAR1 and IFNAR2) lacking a signal peptide are also present in trout. Transfection of HEK 293T cells with the iIFN and iIFNR molecules results in STAT phosphorylation and induction of antiviral genes. These results show that fish possess a functioning iIFN system that may act as a novel defence to combat viral infection
Original languageEnglish
PublisherFigshare
Media of outputOnline
Size1.1MB
Publication statusPublished - 14 Nov 2013

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Virus Diseases
Interferons
Fishes
Protein Sorting Signals
Genes
Antiviral Agents
Trout
HEK293 Cells
Oncorhynchus mykiss
Alternative Splicing
Introns
Transfection
Vertebrates
Phosphorylation

Keywords

  • Interferons
  • means
  • viral disease

Cite this

Xian, C. M. (Author), Zou, J. (Author), Nie, P. (Author), Huang, B. (Author), Yu, Z. (Author), Collet, B. (Author), & Secombes, C. J. (Author). (2013). Intercellular Interferons in Fish: A unique means to combat viral infection. Database, Figshare. Retrieved from http://figshare.com/articles/_Intracellular_Interferons_in_Fish_A_Unique_Means_to_Combat_Viral_Infection_/851266
Intercellular Interferons in Fish : A unique means to combat viral infection. Xian, Chang Ming (Author); Zou, Jun (Author); Nie, Pin (Author); Huang, Bei (Author); Yu, Zhanglong (Author); Collet, Bertrand (Author); Secombes, Christopher John (Author). 2013. Figshare.

Research output: Non-textual formDatabase

Xian, CM, Zou, J, Nie, P, Huang, B, Yu, Z, Collet, B & Secombes, CJ, Intercellular Interferons in Fish: A unique means to combat viral infection, 2013, Database, Figshare.
Xian CM (Author), Zou J (Author), Nie P (Author), Huang B (Author), Yu Z (Author), Collet B (Author) et al. Intercellular Interferons in Fish: A unique means to combat viral infection Figshare. 2013.
Xian, Chang Ming (Author) ; Zou, Jun (Author) ; Nie, Pin (Author) ; Huang, Bei (Author) ; Yu, Zhanglong (Author) ; Collet, Bertrand (Author) ; Secombes, Christopher John (Author). / Intercellular Interferons in Fish : A unique means to combat viral infection. [Database].
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AB - We demonstrate for the first time in vertebrates, that alternative splicing of interferon (IFN) genes can lead to a functional intracellular IFN (iIFN). Fish IFN genes possess introns and in rainbow trout three alternatively spliced transcripts of the IFN1 gene exist. Two of the encoded IFNs are predicted to lack a signal peptide. When overexpressed these iIFNs induce antiviral responses. Variants of the two IFNR receptor chains (IFNAR1 and IFNAR2) lacking a signal peptide are also present in trout. Transfection of HEK 293T cells with the iIFN and iIFNR molecules results in STAT phosphorylation and induction of antiviral genes. These results show that fish possess a functioning iIFN system that may act as a novel defence to combat viral infection

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