Interleukin-1 in fish

C J Secombes, S Bird, C Cunningham, J Zou

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52 Citations (Scopus)

Abstract

It has been known for over a decade that IL-1 bioactivity exists in fish. Recently IL-1 has been cloned in several teleost species using two different approaches; homology cloning or a suppression subtractive PCR technique. One of the most important differences of the fish sequences compared with mammalian IL-1 is the lack of a clear ICE cut site, and it remains to be determined whether the fish molecule is processed to a mature peptide. The IL-1 gene organisation has also been determined for rainbow trout, and Southern blot analysis suggests at least one related gene is present in this species. Expression of the IL-1 transcript can be induced in vitro by stimulation of head kidney leucocytes with LPS or PHA, or in vivo by challenge with Gram-negative bacteria. A number of incompletely spliced transcripts have also been detected in tissues from challenged fish. (C) 1999 Academic Press.

Original languageEnglish
Pages (from-to)335-343
Number of pages9
JournalFish & Shellfish Immunology
Volume9
Publication statusPublished - 1999

Keywords

  • interleukin-1
  • cytokines
  • fish
  • gene
  • RECEPTOR ANTAGONIST
  • CRYSTAL-STRUCTURE
  • IL-1 RECEPTOR
  • GENE FAMILY
  • MOUSE IL-1
  • MACROPHAGES
  • EXPRESSION
  • RESPONSES
  • EVOLUTION
  • CELLS

Cite this

Secombes, C. J., Bird, S., Cunningham, C., & Zou, J. (1999). Interleukin-1 in fish. Fish & Shellfish Immunology, 9, 335-343.