Interleukin-1 in fish

C J Secombes, S Bird, C Cunningham, J Zou

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

It has been known for over a decade that IL-1 bioactivity exists in fish. Recently IL-1 has been cloned in several teleost species using two different approaches; homology cloning or a suppression subtractive PCR technique. One of the most important differences of the fish sequences compared with mammalian IL-1 is the lack of a clear ICE cut site, and it remains to be determined whether the fish molecule is processed to a mature peptide. The IL-1 gene organisation has also been determined for rainbow trout, and Southern blot analysis suggests at least one related gene is present in this species. Expression of the IL-1 transcript can be induced in vitro by stimulation of head kidney leucocytes with LPS or PHA, or in vivo by challenge with Gram-negative bacteria. A number of incompletely spliced transcripts have also been detected in tissues from challenged fish. (C) 1999 Academic Press.

Original languageEnglish
Pages (from-to)335-343
Number of pages9
JournalFish & Shellfish Immunology
Volume9
Publication statusPublished - 1999

Keywords

  • interleukin-1
  • cytokines
  • fish
  • gene
  • RECEPTOR ANTAGONIST
  • CRYSTAL-STRUCTURE
  • IL-1 RECEPTOR
  • GENE FAMILY
  • MOUSE IL-1
  • MACROPHAGES
  • EXPRESSION
  • RESPONSES
  • EVOLUTION
  • CELLS

Cite this

Secombes, C. J., Bird, S., Cunningham, C., & Zou, J. (1999). Interleukin-1 in fish. Fish & Shellfish Immunology, 9, 335-343.

Interleukin-1 in fish. / Secombes, C J ; Bird, S ; Cunningham, C ; Zou, J .

In: Fish & Shellfish Immunology, Vol. 9, 1999, p. 335-343.

Research output: Contribution to journalArticle

Secombes, CJ, Bird, S, Cunningham, C & Zou, J 1999, 'Interleukin-1 in fish', Fish & Shellfish Immunology, vol. 9, pp. 335-343.
Secombes CJ, Bird S, Cunningham C, Zou J. Interleukin-1 in fish. Fish & Shellfish Immunology. 1999;9:335-343.
Secombes, C J ; Bird, S ; Cunningham, C ; Zou, J . / Interleukin-1 in fish. In: Fish & Shellfish Immunology. 1999 ; Vol. 9. pp. 335-343.
@article{adb749f792434fa5a155f3089ffbbad3,
title = "Interleukin-1 in fish",
abstract = "It has been known for over a decade that IL-1 bioactivity exists in fish. Recently IL-1 has been cloned in several teleost species using two different approaches; homology cloning or a suppression subtractive PCR technique. One of the most important differences of the fish sequences compared with mammalian IL-1 is the lack of a clear ICE cut site, and it remains to be determined whether the fish molecule is processed to a mature peptide. The IL-1 gene organisation has also been determined for rainbow trout, and Southern blot analysis suggests at least one related gene is present in this species. Expression of the IL-1 transcript can be induced in vitro by stimulation of head kidney leucocytes with LPS or PHA, or in vivo by challenge with Gram-negative bacteria. A number of incompletely spliced transcripts have also been detected in tissues from challenged fish. (C) 1999 Academic Press.",
keywords = "interleukin-1, cytokines, fish, gene, RECEPTOR ANTAGONIST, CRYSTAL-STRUCTURE, IL-1 RECEPTOR, GENE FAMILY, MOUSE IL-1, MACROPHAGES, EXPRESSION, RESPONSES, EVOLUTION, CELLS",
author = "Secombes, {C J} and S Bird and C Cunningham and J Zou",
year = "1999",
language = "English",
volume = "9",
pages = "335--343",
journal = "Fish & Shellfish Immunology",
issn = "1050-4648",
publisher = "ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD",

}

TY - JOUR

T1 - Interleukin-1 in fish

AU - Secombes, C J

AU - Bird, S

AU - Cunningham, C

AU - Zou, J

PY - 1999

Y1 - 1999

N2 - It has been known for over a decade that IL-1 bioactivity exists in fish. Recently IL-1 has been cloned in several teleost species using two different approaches; homology cloning or a suppression subtractive PCR technique. One of the most important differences of the fish sequences compared with mammalian IL-1 is the lack of a clear ICE cut site, and it remains to be determined whether the fish molecule is processed to a mature peptide. The IL-1 gene organisation has also been determined for rainbow trout, and Southern blot analysis suggests at least one related gene is present in this species. Expression of the IL-1 transcript can be induced in vitro by stimulation of head kidney leucocytes with LPS or PHA, or in vivo by challenge with Gram-negative bacteria. A number of incompletely spliced transcripts have also been detected in tissues from challenged fish. (C) 1999 Academic Press.

AB - It has been known for over a decade that IL-1 bioactivity exists in fish. Recently IL-1 has been cloned in several teleost species using two different approaches; homology cloning or a suppression subtractive PCR technique. One of the most important differences of the fish sequences compared with mammalian IL-1 is the lack of a clear ICE cut site, and it remains to be determined whether the fish molecule is processed to a mature peptide. The IL-1 gene organisation has also been determined for rainbow trout, and Southern blot analysis suggests at least one related gene is present in this species. Expression of the IL-1 transcript can be induced in vitro by stimulation of head kidney leucocytes with LPS or PHA, or in vivo by challenge with Gram-negative bacteria. A number of incompletely spliced transcripts have also been detected in tissues from challenged fish. (C) 1999 Academic Press.

KW - interleukin-1

KW - cytokines

KW - fish

KW - gene

KW - RECEPTOR ANTAGONIST

KW - CRYSTAL-STRUCTURE

KW - IL-1 RECEPTOR

KW - GENE FAMILY

KW - MOUSE IL-1

KW - MACROPHAGES

KW - EXPRESSION

KW - RESPONSES

KW - EVOLUTION

KW - CELLS

M3 - Article

VL - 9

SP - 335

EP - 343

JO - Fish & Shellfish Immunology

JF - Fish & Shellfish Immunology

SN - 1050-4648

ER -