Interleukin-10 expression by real-time PCR and homology modelling analysis in the European sea bass (Dicentrarchus Labrax L.).

F. Buonocore, E. Randelli, Steven Bird, Christopher John Secombes, A. Facchiano, S. Costantini, Giuseppe Scapigliati

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Abstract

In this paper we investigate the interleukin-10 (IL-10) expression and 3D modelling of the European sea bass (Dicentrarchus labrax L.). IL-10 is a regulatory cytokine that has been intensively studied in mammals and has been found mainly involved in the suppression or deactivation of activated immune responses. The full-length sea bass IL-10 cDNA consists of 1015 bp and is translated in a predicted molecule of 187 amino acids. A multiple alignment of this peptide with other known sequences showed the conservation of fundamental IL-10 family characteristics. Expression analysis by real-time PCR in control fish showed a high basal expression in the head kidney (HK), followed by gut and brain. In vitro treatment of HK leucocytes with LPS showed an intense increase of IL-10 expression at 4 h and a significant decrease at 24 h, with PHA-L no differences were evidenced. A 3D model for sea bass IL-10 was obtained by accurate homology procedures and putative interaction residues with the IL-10 receptor described. The results suggested that sea bass IL-10 could be involved in anti-inflammatory responses, but further experiments are needed to assess its importance in response to pathogenic agents, vaccinations and immunostimulants. (C) 2007 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)512-522
Number of pages10
JournalAquaculture
Volume270
Issue number1-4
DOIs
Publication statusPublished - 28 Sep 2007

Keywords

  • interleukin-10 (IL-10)
  • European sea bass
  • Dicentrarchus labrax
  • expression analysis
  • real-time PCR
  • 3D modelling
  • molecular recognition
  • secondary structure
  • cytokine production
  • crystal-structure
  • interferon-gamma
  • T-cells
  • protein
  • cloning
  • IL-10
  • receptor

Cite this

Buonocore, F., Randelli, E., Bird, S., Secombes, C. J., Facchiano, A., Costantini, S., & Scapigliati, G. (2007). Interleukin-10 expression by real-time PCR and homology modelling analysis in the European sea bass (Dicentrarchus Labrax L.). Aquaculture, 270(1-4), 512-522. https://doi.org/10.1016/j.aquaculture.2007.05.040