Interleukin-10 expression by real-time PCR and homology modelling analysis in the European sea bass (Dicentrarchus Labrax L.).

F. Buonocore, E. Randelli, Steven Bird, Christopher John Secombes, A. Facchiano, S. Costantini, Giuseppe Scapigliati

Research output: Contribution to journalArticle

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Abstract

In this paper we investigate the interleukin-10 (IL-10) expression and 3D modelling of the European sea bass (Dicentrarchus labrax L.). IL-10 is a regulatory cytokine that has been intensively studied in mammals and has been found mainly involved in the suppression or deactivation of activated immune responses. The full-length sea bass IL-10 cDNA consists of 1015 bp and is translated in a predicted molecule of 187 amino acids. A multiple alignment of this peptide with other known sequences showed the conservation of fundamental IL-10 family characteristics. Expression analysis by real-time PCR in control fish showed a high basal expression in the head kidney (HK), followed by gut and brain. In vitro treatment of HK leucocytes with LPS showed an intense increase of IL-10 expression at 4 h and a significant decrease at 24 h, with PHA-L no differences were evidenced. A 3D model for sea bass IL-10 was obtained by accurate homology procedures and putative interaction residues with the IL-10 receptor described. The results suggested that sea bass IL-10 could be involved in anti-inflammatory responses, but further experiments are needed to assess its importance in response to pathogenic agents, vaccinations and immunostimulants. (C) 2007 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)512-522
Number of pages10
JournalAquaculture
Volume270
Issue number1-4
DOIs
Publication statusPublished - 28 Sep 2007

Keywords

  • interleukin-10 (IL-10)
  • European sea bass
  • Dicentrarchus labrax
  • expression analysis
  • real-time PCR
  • 3D modelling
  • molecular recognition
  • secondary structure
  • cytokine production
  • crystal-structure
  • interferon-gamma
  • T-cells
  • protein
  • cloning
  • IL-10
  • receptor

Cite this

Interleukin-10 expression by real-time PCR and homology modelling analysis in the European sea bass (Dicentrarchus Labrax L.).. / Buonocore, F.; Randelli, E.; Bird, Steven; Secombes, Christopher John; Facchiano, A.; Costantini, S.; Scapigliati, Giuseppe.

In: Aquaculture, Vol. 270, No. 1-4, 28.09.2007, p. 512-522.

Research output: Contribution to journalArticle

Buonocore, F. ; Randelli, E. ; Bird, Steven ; Secombes, Christopher John ; Facchiano, A. ; Costantini, S. ; Scapigliati, Giuseppe. / Interleukin-10 expression by real-time PCR and homology modelling analysis in the European sea bass (Dicentrarchus Labrax L.). In: Aquaculture. 2007 ; Vol. 270, No. 1-4. pp. 512-522.
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