Abstract
In this paper we investigate the interleukin-10 (IL-10) expression and 3D modelling of the European sea bass (Dicentrarchus labrax L.). IL-10 is a regulatory cytokine that has been intensively studied in mammals and has been found mainly involved in the suppression or deactivation of activated immune responses. The full-length sea bass IL-10 cDNA consists of 1015 bp and is translated in a predicted molecule of 187 amino acids. A multiple alignment of this peptide with other known sequences showed the conservation of fundamental IL-10 family characteristics. Expression analysis by real-time PCR in control fish showed a high basal expression in the head kidney (HK), followed by gut and brain. In vitro treatment of HK leucocytes with LPS showed an intense increase of IL-10 expression at 4 h and a significant decrease at 24 h, with PHA-L no differences were evidenced. A 3D model for sea bass IL-10 was obtained by accurate homology procedures and putative interaction residues with the IL-10 receptor described. The results suggested that sea bass IL-10 could be involved in anti-inflammatory responses, but further experiments are needed to assess its importance in response to pathogenic agents, vaccinations and immunostimulants. (C) 2007 Elsevier B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 512-522 |
Number of pages | 10 |
Journal | Aquaculture |
Volume | 270 |
Issue number | 1-4 |
DOIs | |
Publication status | Published - 28 Sept 2007 |
Keywords
- interleukin-10 (IL-10)
- European sea bass
- Dicentrarchus labrax
- expression analysis
- real-time PCR
- 3D modelling
- molecular recognition
- secondary structure
- cytokine production
- crystal-structure
- interferon-gamma
- T-cells
- protein
- cloning
- IL-10
- receptor