INTERLEUKIN-5 ENHANCES THE INVITRO ADHESION OF HUMAN EOSINOPHILS, BUT NOT NEUTROPHILS, IN A LEUKOCYTE INTEGRIN (CD11/18)-DEPENDENT MANNER

Garry Michael Walsh, A J WARDLAW, A HARTNELL, C J SANDERSON, A B KAY

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Abstract

Interleukin (IL-5) was found to enhance the adhesion of eosinophils, but not neutrophils, to both microvascular and large vein endothelial cells in a dose-dependent manner. Granulocyte/macrophage-colony-stimulating factor (GM-CSF) and platelet-activating factor (PAF) enhanced both eosinophil and neutrophil adhesion. Significant increases in eosinophil CR3 expression, but not LFA-1, were observed following pre-incubation with PAF, IL-3, IL-5 or GM-CSF. Neutrophil CR3 expression was increased significantly by pre-incubation with PAF or GM-CSF, but not IL-3 or IL-5. Enhanced adhesion to human microvascular endothelial cells (HMVEC) or human umbilical vein endothelial cells (HUVEC) was inhibited by (ranked in order of potency) anti-CR3-alpha = common beta-chain > LFA-1-alpha. Anti-p150,95-alpha had no measurable effect. Basal expression of eosinophil CR3 with monoclonal antibody inhibited IL-5-induced eosinophil hyperadherence to HUVEC in a manner almost identical to inhibition in the presence of excess anti-CR3. Thus, a conformational or affintiy change in adhesion receptors following activation seems more important than a simple increase in numbers. No inhibition of unstimulated eosinophil adhesion to HMVEC or HUVEC by CD11/18 monoclonal antibody was observed. These findings demonstrate that IL-5 enhances eosinophil, but not neutrophil, adherence reactions, by a mechanism dependent, at least in part, on the CD11/18 family of adhesion glycoproteins.

Original languageEnglish
Pages (from-to)174-178
Number of pages5
JournalInternational Archives of Allergy and Applied Immunology
Volume94
Issue number1-4
Publication statusPublished - 1991

Keywords

  • PLATELET-ACTIVATING FACTOR
  • VASCULAR ENDOTHELIAL-CELLS
  • EXPRESSION
  • LEUKOCYTE
  • ADHERENCE
  • ICAM-1
  • LFA-1
  • RECEPTORS
  • P150,95
  • TISSUE

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