Interrupted catalytic domain structures in xylanases from two distantly related strains of Prevotella ruminicola

H J  Flint; T R  Whitehead; J C  Martin; A  Gasparic

Interrupted catalytic domain structures in xylanases from two distantly related strains of Prevotella ruminicola

H J Flint, T R Whitehead, J C Martin, A Gasparic

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Two xylanases from the rumen anaerobic bacterium Prevotella ruminicola were found to possess highly unusual structures in which family 10 catalytic domains are interrupted by unrelated sequences. XynC from P. ruminicola B(1)4 carries a 160 amino-acid insertion, while a P. ruminicola D31d xylanase carries an unrelated region of 280 amino acids, containing an imperfect 130 amino-acid duplication. Both regions of family 10 similarity were shown to be essential for activity of the D31d enzyme.

Original language	English
Pages (from-to)	161-165
Number of pages	5
Journal	Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology
Volume	1337
Issue number	2
Publication status	Published - 8 Feb 1997

Keywords

xylanase
rumen
domain structure
interrupting sequence
(P-ruminicola)
AMINO-ACID-SEQUENCE
BACTEROIDES-RUMINICOLA
PSEUDOMONAS-FLUORESCENS
CELLULOMONAS-FIMI
STREPTOMYCES-LIVIDANS
ENDOGLUCANASE GENE
CRYSTAL-STRUCTURE
ACTIVE-SITE
BACTERIA
FAMILIES

Cite this

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title = "Interrupted catalytic domain structures in xylanases from two distantly related strains of Prevotella ruminicola",

abstract = "Two xylanases from the rumen anaerobic bacterium Prevotella ruminicola were found to possess highly unusual structures in which family 10 catalytic domains are interrupted by unrelated sequences. XynC from P. ruminicola B(1)4 carries a 160 amino-acid insertion, while a P. ruminicola D31d xylanase carries an unrelated region of 280 amino acids, containing an imperfect 130 amino-acid duplication. Both regions of family 10 similarity were shown to be essential for activity of the D31d enzyme.",

keywords = "xylanase, rumen, domain structure, interrupting sequence, (P-ruminicola), AMINO-ACID-SEQUENCE, BACTEROIDES-RUMINICOLA, PSEUDOMONAS-FLUORESCENS, CELLULOMONAS-FIMI, STREPTOMYCES-LIVIDANS, ENDOGLUCANASE GENE, CRYSTAL-STRUCTURE, ACTIVE-SITE, BACTERIA, FAMILIES",

author = "Flint, {H J} and Whitehead, {T R} and Martin, {J C} and A Gasparic",

year = "1997",

month = feb,

day = "8",

language = "English",

volume = "1337",

pages = "161--165",

journal = "Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology",

issn = "0167-4838",

publisher = "Elsevier BV",

number = "2",

}

TY - JOUR

T1 - Interrupted catalytic domain structures in xylanases from two distantly related strains of Prevotella ruminicola

AU - Flint, H J

AU - Whitehead, T R

AU - Martin, J C

AU - Gasparic, A

PY - 1997/2/8

Y1 - 1997/2/8

N2 - Two xylanases from the rumen anaerobic bacterium Prevotella ruminicola were found to possess highly unusual structures in which family 10 catalytic domains are interrupted by unrelated sequences. XynC from P. ruminicola B(1)4 carries a 160 amino-acid insertion, while a P. ruminicola D31d xylanase carries an unrelated region of 280 amino acids, containing an imperfect 130 amino-acid duplication. Both regions of family 10 similarity were shown to be essential for activity of the D31d enzyme.

AB - Two xylanases from the rumen anaerobic bacterium Prevotella ruminicola were found to possess highly unusual structures in which family 10 catalytic domains are interrupted by unrelated sequences. XynC from P. ruminicola B(1)4 carries a 160 amino-acid insertion, while a P. ruminicola D31d xylanase carries an unrelated region of 280 amino acids, containing an imperfect 130 amino-acid duplication. Both regions of family 10 similarity were shown to be essential for activity of the D31d enzyme.

KW - xylanase

KW - rumen

KW - domain structure

KW - interrupting sequence

KW - (P-ruminicola)

KW - AMINO-ACID-SEQUENCE

KW - BACTEROIDES-RUMINICOLA

KW - PSEUDOMONAS-FLUORESCENS

KW - CELLULOMONAS-FIMI

KW - STREPTOMYCES-LIVIDANS

KW - ENDOGLUCANASE GENE

KW - CRYSTAL-STRUCTURE

KW - ACTIVE-SITE

KW - BACTERIA

KW - FAMILIES

M3 - Article

VL - 1337

SP - 161

EP - 165

JO - Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

JF - Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

SN - 0167-4838

IS - 2

ER -

Interrupted catalytic domain structures in xylanases from two distantly related strains of Prevotella ruminicola

Abstract

Keywords

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