Intestinal hydrolysis and microbial biotransformation of diacetoxyscirpenol-α-glucoside, HT-2-β-glucoside and N-(1-deoxy-d-fructos-1-yl) fumonisin B1 by human gut microbiota in vitro

Noshin Daud, Valerie Currie, Gary Duncan, Mark Busman, Silvia W. Gratz* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Fusarium mycotoxins are common contaminants in cereals and often co-occur with plant-derived mycotoxin sugar conjugates. Several of these modified mycotoxins are not degraded in the small intestine and hence carried through to the large intestine where microbial transformation may occur. This study aims to assess the gastrointestinal stability of the trichothecenes HT-2 toxin (HT-2), HT-2-β-glucoside (HT-2-Glc), diacetoxyscirpenol (DAS), DAS-α-glucoside (DAS-Glc) and fumonisin B1 (FB1), N-(1-deoxy-d-fructos-1-yl) fumonisin-B1 (NDF-FB1). All tested modified mycotoxins were stable under upper gastrointestinal (GI) conditions. In faecal batch culture experiments, HT-2-Glc was hydrolysed efficiently and no further microbial biotransformation of HT-2 was observed. DAS-Glc hydrolysis was slow and DAS was de-acetylated to 15-monoacetoxyscripenol. NDF-FB1 was hydrolysed at the slowest rate and FB1 accumulation varied between donor samples. Our results demonstrate that all tested modified mycotoxins are stable in the upper GI tract and efficiently hydrolysed by human gut microbiota, thus potentially contributing to colonic toxicity. Hence the microbial biotransformation of any novel modified mycotoxins needs to be carefully evaluated.
Original languageEnglish
Number of pages9
JournalInternational Journal of Food Sciences and Nutrition
Early online date3 Dec 2019
DOIs
Publication statusE-pub ahead of print - 3 Dec 2019

Fingerprint

diacetoxyscirpenol
fumonisin B1
Mycotoxins
Glucosides
Biotransformation
biotransformation
intestinal microorganisms
mycotoxins
glucosides
Hydrolysis
hydrolysis
HT-2 toxin
Trichothecenes
Upper Gastrointestinal Tract
Batch Cell Culture Techniques
trichothecenes
Large Intestine
Fusarium
large intestine
Small Intestine

Keywords

  • Diacetoxyscirpenol-α-glucoside
  • HT-2-β-glucoside
  • N-(1-deoxy-d-fructos-1-yl) fumonisin B1
  • human faecal microbiota
  • masked mycotoxins
  • release
  • APPROPRIATENESS
  • DEOXYNIVALENOL-3-GLUCOSIDE
  • STABILITY
  • FUSARIUM
  • FATE
  • HT-2-beta-glucoside
  • Diacetoxyscirpenol-alpha-glucoside
  • TRICHOTHECENE MYCOTOXINS
  • GUIDANCE VALUE
  • METABOLISM
  • MASKED MYCOTOXINS
  • T-2 TOXIN

Cite this

@article{6eb4beb3497141b8aa6c0ccbc1561fdf,
title = "Intestinal hydrolysis and microbial biotransformation of diacetoxyscirpenol-α-glucoside, HT-2-β-glucoside and N-(1-deoxy-d-fructos-1-yl) fumonisin B1 by human gut microbiota in vitro",
abstract = "Fusarium mycotoxins are common contaminants in cereals and often co-occur with plant-derived mycotoxin sugar conjugates. Several of these modified mycotoxins are not degraded in the small intestine and hence carried through to the large intestine where microbial transformation may occur. This study aims to assess the gastrointestinal stability of the trichothecenes HT-2 toxin (HT-2), HT-2-β-glucoside (HT-2-Glc), diacetoxyscirpenol (DAS), DAS-α-glucoside (DAS-Glc) and fumonisin B1 (FB1), N-(1-deoxy-d-fructos-1-yl) fumonisin-B1 (NDF-FB1). All tested modified mycotoxins were stable under upper gastrointestinal (GI) conditions. In faecal batch culture experiments, HT-2-Glc was hydrolysed efficiently and no further microbial biotransformation of HT-2 was observed. DAS-Glc hydrolysis was slow and DAS was de-acetylated to 15-monoacetoxyscripenol. NDF-FB1 was hydrolysed at the slowest rate and FB1 accumulation varied between donor samples. Our results demonstrate that all tested modified mycotoxins are stable in the upper GI tract and efficiently hydrolysed by human gut microbiota, thus potentially contributing to colonic toxicity. Hence the microbial biotransformation of any novel modified mycotoxins needs to be carefully evaluated.",
keywords = "Diacetoxyscirpenol-α-glucoside, HT-2-β-glucoside, N-(1-deoxy-d-fructos-1-yl) fumonisin B1, human faecal microbiota, masked mycotoxins, release, APPROPRIATENESS, DEOXYNIVALENOL-3-GLUCOSIDE, STABILITY, FUSARIUM, FATE, HT-2-beta-glucoside, Diacetoxyscirpenol-alpha-glucoside, TRICHOTHECENE MYCOTOXINS, GUIDANCE VALUE, METABOLISM, MASKED MYCOTOXINS, T-2 TOXIN",
author = "Noshin Daud and Valerie Currie and Gary Duncan and Mark Busman and Gratz, {Silvia W.}",
note = "Acknowledgements In Peoria, the following colleagues were involved in the preparation of the modified mycotoxins: S McCormick, C Maragos, K Sieve, K Wetterhorn. Funding This study was funded by Scottish Government Rural, and Environment Science, and Analytical Services Division (RESAS) [RG13488-33]. ND was supported by an Elphinstone PhD scholarship by the University of Aberdeen.",
year = "2019",
month = "12",
day = "3",
doi = "10.1080/09637486.2019.1698015",
language = "English",
journal = "International Journal of Food Sciences and Nutrition",
issn = "0963-7486",
publisher = "Taylor & Francis",

}

TY - JOUR

T1 - Intestinal hydrolysis and microbial biotransformation of diacetoxyscirpenol-α-glucoside, HT-2-β-glucoside and N-(1-deoxy-d-fructos-1-yl) fumonisin B1 by human gut microbiota in vitro

AU - Daud, Noshin

AU - Currie, Valerie

AU - Duncan, Gary

AU - Busman, Mark

AU - Gratz, Silvia W.

N1 - Acknowledgements In Peoria, the following colleagues were involved in the preparation of the modified mycotoxins: S McCormick, C Maragos, K Sieve, K Wetterhorn. Funding This study was funded by Scottish Government Rural, and Environment Science, and Analytical Services Division (RESAS) [RG13488-33]. ND was supported by an Elphinstone PhD scholarship by the University of Aberdeen.

PY - 2019/12/3

Y1 - 2019/12/3

N2 - Fusarium mycotoxins are common contaminants in cereals and often co-occur with plant-derived mycotoxin sugar conjugates. Several of these modified mycotoxins are not degraded in the small intestine and hence carried through to the large intestine where microbial transformation may occur. This study aims to assess the gastrointestinal stability of the trichothecenes HT-2 toxin (HT-2), HT-2-β-glucoside (HT-2-Glc), diacetoxyscirpenol (DAS), DAS-α-glucoside (DAS-Glc) and fumonisin B1 (FB1), N-(1-deoxy-d-fructos-1-yl) fumonisin-B1 (NDF-FB1). All tested modified mycotoxins were stable under upper gastrointestinal (GI) conditions. In faecal batch culture experiments, HT-2-Glc was hydrolysed efficiently and no further microbial biotransformation of HT-2 was observed. DAS-Glc hydrolysis was slow and DAS was de-acetylated to 15-monoacetoxyscripenol. NDF-FB1 was hydrolysed at the slowest rate and FB1 accumulation varied between donor samples. Our results demonstrate that all tested modified mycotoxins are stable in the upper GI tract and efficiently hydrolysed by human gut microbiota, thus potentially contributing to colonic toxicity. Hence the microbial biotransformation of any novel modified mycotoxins needs to be carefully evaluated.

AB - Fusarium mycotoxins are common contaminants in cereals and often co-occur with plant-derived mycotoxin sugar conjugates. Several of these modified mycotoxins are not degraded in the small intestine and hence carried through to the large intestine where microbial transformation may occur. This study aims to assess the gastrointestinal stability of the trichothecenes HT-2 toxin (HT-2), HT-2-β-glucoside (HT-2-Glc), diacetoxyscirpenol (DAS), DAS-α-glucoside (DAS-Glc) and fumonisin B1 (FB1), N-(1-deoxy-d-fructos-1-yl) fumonisin-B1 (NDF-FB1). All tested modified mycotoxins were stable under upper gastrointestinal (GI) conditions. In faecal batch culture experiments, HT-2-Glc was hydrolysed efficiently and no further microbial biotransformation of HT-2 was observed. DAS-Glc hydrolysis was slow and DAS was de-acetylated to 15-monoacetoxyscripenol. NDF-FB1 was hydrolysed at the slowest rate and FB1 accumulation varied between donor samples. Our results demonstrate that all tested modified mycotoxins are stable in the upper GI tract and efficiently hydrolysed by human gut microbiota, thus potentially contributing to colonic toxicity. Hence the microbial biotransformation of any novel modified mycotoxins needs to be carefully evaluated.

KW - Diacetoxyscirpenol-α-glucoside

KW - HT-2-β-glucoside

KW - N-(1-deoxy-d-fructos-1-yl) fumonisin B1

KW - human faecal microbiota

KW - masked mycotoxins

KW - release

KW - APPROPRIATENESS

KW - DEOXYNIVALENOL-3-GLUCOSIDE

KW - STABILITY

KW - FUSARIUM

KW - FATE

KW - HT-2-beta-glucoside

KW - Diacetoxyscirpenol-alpha-glucoside

KW - TRICHOTHECENE MYCOTOXINS

KW - GUIDANCE VALUE

KW - METABOLISM

KW - MASKED MYCOTOXINS

KW - T-2 TOXIN

U2 - 10.1080/09637486.2019.1698015

DO - 10.1080/09637486.2019.1698015

M3 - Article

C2 - 31791183

JO - International Journal of Food Sciences and Nutrition

JF - International Journal of Food Sciences and Nutrition

SN - 0963-7486

ER -