Intragenic meiotic recombination in Schizosaccharomyces pombe is sensitive to environmental temperature changes

Simon D. Brown, Charlotte Audoynaud, Alexander Lorenz* (Corresponding Author)

*Corresponding author for this work

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Abstract

Changes in environmental temperature influence cellular processes and their dynamics and thus affect the life cycle of organisms that are unable to control their cell/body temperature. Meiotic recombination is the cellular process essential for producing healthy haploid gametes by providing physical links (chiasmata) between homologous chromosomes to guide their accurate segregation. Additionally, meiotic recombination – initiated by programmed DNA double-strand breaks (DSBs) – can generate genetic diversity and, therefore, is a driving force of evolution. Environmental temperature influencing meiotic recombination outcome thus may be a crucial determinant of reproductive success and genetic diversity. Indeed, meiotic recombination frequency in fungi, plants, and invertebrates changes with temperature. In most organisms these temperature-induced changes in meiotic recombination seem to be mediated through the meiosis-specific chromosome axis organization, the synaptonemal complex in particular. The fission yeast Schizosaccharomyces pombe does not possess a synaptonemal complex. Thus, we tested how environmental temperature modulates meiotic recombination frequency in the absence of a fully-fledged synaptonemal complex. We show that intragenic recombination (gene conversion) positively correlates with temperature within a certain range, especially at meiotic recombination hotspots. In contrast, crossover recombination, which manifests itself as chiasmata, is less affected. Based on our observations, we suggest that, in addition to changes in DSB frequency, DSB processing could be another temperature-sensitive step causing temperature-induced recombination rate alterations.
Original languageEnglish
Pages (from-to)195-207
Number of pages13
JournalChromosome Research
Volume28
Early online date17 Apr 2020
DOIs
Publication statusPublished - Jun 2020

Keywords

  • Schizosaccharomyces pombe
  • meiosis
  • recombination frequency
  • environmental temperature
  • fertile range
  • meiotic recombination
  • FISSION YEAST
  • LINEAR ELEMENTS
  • SYNAPTONEMAL COMPLEX
  • CROSSOVER HOMEOSTASIS
  • CHROMOSOME
  • CONSERVATION
  • PARTNER CHOICE
  • MEIOSIS
  • DNA BREAKAGE
  • ELEVATED-TEMPERATURE

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