Involvement of CCR5 in the passage of Th-1-type cells across the blood-retina barrier in experimental autoimmune uveitis

Isabel Joan Crane, Heping Xu, Carol Ann Wallace, Ayyakkannu Manivannan, M. Mack, Janet Mary Liversidge, G. Marquez, Peter Frederick Sharp, John Vincent Forrester

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Although the recruitment of T helper cell type I (Th1)/Th2 cells into peripheral tissues is essential for inflammation and the host response to infection, the traffic signals that enable the distinct positioning of Th1/Th2 cells are unclear. We have determined the role of CC chemokine receptor 5 (CCR5) in this using experimental antoimmune uveitis (EAU) as a model system. In EAU, Th1-like cells are preferentially recruited into the retina across the blood-retina barrier, partly as a result of expression of the adhesion molecules P-selectin glycoprotein ligand I and lymphocyte function-associated antigen-1 on these cells. CD3+ T cells, infiltrating the retina, also expressed the chemokine receptor CCR5, and CCR5 ligands, macrophage-inflammatory protein-1 alpha (MIP-1 alpha), MIP-1 beta, and regulated on activation, normal T expressed and secreted (RANTES), were strongly expressed in the retina at peak EAU. Th1-like cells, polarized in vitro, expressed high levels of CCR5. The trafficking of these CCR5(+) cells was examined by tracking them after adoptive transfer in real time in vivo at an early disease stage using scanning laser ophthalmoscopy. Treatment of the cells with antibody against CCR5 prior to transfer resulted in a reduction in their infiltration into the retina. However, rolling velocity, rolling efficiency, and adherence of the cells to retinal endothelium were not reduced. CCR5 is clearly important for Th1 cell recruitment, and this study demonstrates for the first time in vivo that CCR5 may act at the level of transendothelial migration rather than at the earlier stage of rolling on the endothelium.

Original languageEnglish
Pages (from-to)435-443
Number of pages8
JournalJournal of Leukocyte Biology
Volume79
Issue number3
DOIs
Publication statusPublished - Mar 2006

Keywords

  • chemokines
  • chemokine receptor
  • cell trafficking
  • inflammation
  • CHEMOKINE RECEPTOR EXPRESSION
  • IN-VIVO
  • CUTTING EDGE
  • TH1 CELLS
  • LEUKOCYTE TRAFFICKING
  • T-HELPER-2 CELLS
  • UP-REGULATION
  • T-CELLS
  • MIGRATION
  • RECRUITMENT

Cite this

Involvement of CCR5 in the passage of Th-1-type cells across the blood-retina barrier in experimental autoimmune uveitis. / Crane, Isabel Joan; Xu, Heping; Wallace, Carol Ann; Manivannan, Ayyakkannu; Mack, M.; Liversidge, Janet Mary; Marquez, G.; Sharp, Peter Frederick; Forrester, John Vincent.

In: Journal of Leukocyte Biology, Vol. 79, No. 3, 03.2006, p. 435-443.

Research output: Contribution to journalArticle

Crane, Isabel Joan ; Xu, Heping ; Wallace, Carol Ann ; Manivannan, Ayyakkannu ; Mack, M. ; Liversidge, Janet Mary ; Marquez, G. ; Sharp, Peter Frederick ; Forrester, John Vincent. / Involvement of CCR5 in the passage of Th-1-type cells across the blood-retina barrier in experimental autoimmune uveitis. In: Journal of Leukocyte Biology. 2006 ; Vol. 79, No. 3. pp. 435-443.
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abstract = "Although the recruitment of T helper cell type I (Th1)/Th2 cells into peripheral tissues is essential for inflammation and the host response to infection, the traffic signals that enable the distinct positioning of Th1/Th2 cells are unclear. We have determined the role of CC chemokine receptor 5 (CCR5) in this using experimental antoimmune uveitis (EAU) as a model system. In EAU, Th1-like cells are preferentially recruited into the retina across the blood-retina barrier, partly as a result of expression of the adhesion molecules P-selectin glycoprotein ligand I and lymphocyte function-associated antigen-1 on these cells. CD3+ T cells, infiltrating the retina, also expressed the chemokine receptor CCR5, and CCR5 ligands, macrophage-inflammatory protein-1 alpha (MIP-1 alpha), MIP-1 beta, and regulated on activation, normal T expressed and secreted (RANTES), were strongly expressed in the retina at peak EAU. Th1-like cells, polarized in vitro, expressed high levels of CCR5. The trafficking of these CCR5(+) cells was examined by tracking them after adoptive transfer in real time in vivo at an early disease stage using scanning laser ophthalmoscopy. Treatment of the cells with antibody against CCR5 prior to transfer resulted in a reduction in their infiltration into the retina. However, rolling velocity, rolling efficiency, and adherence of the cells to retinal endothelium were not reduced. CCR5 is clearly important for Th1 cell recruitment, and this study demonstrates for the first time in vivo that CCR5 may act at the level of transendothelial migration rather than at the earlier stage of rolling on the endothelium.",
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AU - Xu, Heping

AU - Wallace, Carol Ann

AU - Manivannan, Ayyakkannu

AU - Mack, M.

AU - Liversidge, Janet Mary

AU - Marquez, G.

AU - Sharp, Peter Frederick

AU - Forrester, John Vincent

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AB - Although the recruitment of T helper cell type I (Th1)/Th2 cells into peripheral tissues is essential for inflammation and the host response to infection, the traffic signals that enable the distinct positioning of Th1/Th2 cells are unclear. We have determined the role of CC chemokine receptor 5 (CCR5) in this using experimental antoimmune uveitis (EAU) as a model system. In EAU, Th1-like cells are preferentially recruited into the retina across the blood-retina barrier, partly as a result of expression of the adhesion molecules P-selectin glycoprotein ligand I and lymphocyte function-associated antigen-1 on these cells. CD3+ T cells, infiltrating the retina, also expressed the chemokine receptor CCR5, and CCR5 ligands, macrophage-inflammatory protein-1 alpha (MIP-1 alpha), MIP-1 beta, and regulated on activation, normal T expressed and secreted (RANTES), were strongly expressed in the retina at peak EAU. Th1-like cells, polarized in vitro, expressed high levels of CCR5. The trafficking of these CCR5(+) cells was examined by tracking them after adoptive transfer in real time in vivo at an early disease stage using scanning laser ophthalmoscopy. Treatment of the cells with antibody against CCR5 prior to transfer resulted in a reduction in their infiltration into the retina. However, rolling velocity, rolling efficiency, and adherence of the cells to retinal endothelium were not reduced. CCR5 is clearly important for Th1 cell recruitment, and this study demonstrates for the first time in vivo that CCR5 may act at the level of transendothelial migration rather than at the earlier stage of rolling on the endothelium.

KW - chemokines

KW - chemokine receptor

KW - cell trafficking

KW - inflammation

KW - CHEMOKINE RECEPTOR EXPRESSION

KW - IN-VIVO

KW - CUTTING EDGE

KW - TH1 CELLS

KW - LEUKOCYTE TRAFFICKING

KW - T-HELPER-2 CELLS

KW - UP-REGULATION

KW - T-CELLS

KW - MIGRATION

KW - RECRUITMENT

U2 - 10.1189/jlb.0305130

DO - 10.1189/jlb.0305130

M3 - Article

VL - 79

SP - 435

EP - 443

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

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ER -