Involvement of CD44 in leukocyte trafficking at the blood-retinal barrier

Heping Xu, Ayyakkannu Manivannan, Janet Mary Liversidge, Peter Frederick Sharp, John Vincent Forrester, Isabel Joan Crane

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In the present study, we investigated the involvement of CD44 in leukocyte trafficking in vivo at the blood-retinal barrier using experimental autoimmune uveoretinitis (EAU) as a model system. Leukocyte trafficking was evaluated using adoptive transfer of calcein-AM (C-AM)-labeled spleen cells harvested from syngeneic mice at pre-peak severity of EAU to mice at a similar stage of disease. CD44 and its ligand hyaluronan were upregulated in the eye during EAU. CD44-positive leukocytes were found sticking in the retinal venules and postcapillary venules but not in the retinal arterioles nor in mesenteric vessels. Preincubation of in vitro GAM-labeled leukocytes with anti-CD44 monoclonal antibodies (mAb; IM7) or high molecular weight hyaluronic acid (HA) before transfer significantly suppressed leukocyte rolling but not sticking in retinal venules and also reduced cell infiltration in the retinal parenchyma. Administration of the HA-specific enzyme hyaluronidase to mice before cell transfer also reduced leukocyte infiltration, suggesting that CD44-HA interactions are involved in leukocyte recruitment in EAU. This was further supported by the observation that disease severity was reduced by administration of anti-CD44 mAb (M) at the early leukocyte-infiltration stage. Further studies also indicated that CD44 activation was associated with increased levels of apoptosis, and this may also he in part responsible for the reduction in disease severity. These findings demonstrate that CD44 is directly involved in leukocyte-endothelial interaction in vivo and influence the trafficking of primed leukocytes to the retina and their overall survival.

Original languageEnglish
Pages (from-to)1133-1141
Number of pages8
JournalJournal of Leukocyte Biology
Volume72
Issue number6
Publication statusPublished - Dec 2002

Keywords

  • adhesion molecule
  • hyaluronic acid
  • inflammation
  • experimental autoimmune uveoretinitis
  • apoptosis
  • EXPERIMENTAL AUTOIMMUNE UVEORETINITIS
  • ANTI-CD44 TREATMENT
  • HYALURONAN
  • ARTHRITIS
  • ADHESION
  • RECEPTOR
  • CELLS
  • EXTRAVASATION
  • ANTIBODIES
  • ACTIVATION

Cite this

Involvement of CD44 in leukocyte trafficking at the blood-retinal barrier. / Xu, Heping; Manivannan, Ayyakkannu; Liversidge, Janet Mary; Sharp, Peter Frederick; Forrester, John Vincent; Crane, Isabel Joan.

In: Journal of Leukocyte Biology, Vol. 72, No. 6, 12.2002, p. 1133-1141.

Research output: Contribution to journalArticle

Xu, H, Manivannan, A, Liversidge, JM, Sharp, PF, Forrester, JV & Crane, IJ 2002, 'Involvement of CD44 in leukocyte trafficking at the blood-retinal barrier', Journal of Leukocyte Biology, vol. 72, no. 6, pp. 1133-1141.
Xu, Heping ; Manivannan, Ayyakkannu ; Liversidge, Janet Mary ; Sharp, Peter Frederick ; Forrester, John Vincent ; Crane, Isabel Joan. / Involvement of CD44 in leukocyte trafficking at the blood-retinal barrier. In: Journal of Leukocyte Biology. 2002 ; Vol. 72, No. 6. pp. 1133-1141.
@article{d58c113dbf1a4b628f479a5442be0b9e,
title = "Involvement of CD44 in leukocyte trafficking at the blood-retinal barrier",
abstract = "In the present study, we investigated the involvement of CD44 in leukocyte trafficking in vivo at the blood-retinal barrier using experimental autoimmune uveoretinitis (EAU) as a model system. Leukocyte trafficking was evaluated using adoptive transfer of calcein-AM (C-AM)-labeled spleen cells harvested from syngeneic mice at pre-peak severity of EAU to mice at a similar stage of disease. CD44 and its ligand hyaluronan were upregulated in the eye during EAU. CD44-positive leukocytes were found sticking in the retinal venules and postcapillary venules but not in the retinal arterioles nor in mesenteric vessels. Preincubation of in vitro GAM-labeled leukocytes with anti-CD44 monoclonal antibodies (mAb; IM7) or high molecular weight hyaluronic acid (HA) before transfer significantly suppressed leukocyte rolling but not sticking in retinal venules and also reduced cell infiltration in the retinal parenchyma. Administration of the HA-specific enzyme hyaluronidase to mice before cell transfer also reduced leukocyte infiltration, suggesting that CD44-HA interactions are involved in leukocyte recruitment in EAU. This was further supported by the observation that disease severity was reduced by administration of anti-CD44 mAb (M) at the early leukocyte-infiltration stage. Further studies also indicated that CD44 activation was associated with increased levels of apoptosis, and this may also he in part responsible for the reduction in disease severity. These findings demonstrate that CD44 is directly involved in leukocyte-endothelial interaction in vivo and influence the trafficking of primed leukocytes to the retina and their overall survival.",
keywords = "adhesion molecule, hyaluronic acid, inflammation, experimental autoimmune uveoretinitis, apoptosis, EXPERIMENTAL AUTOIMMUNE UVEORETINITIS, ANTI-CD44 TREATMENT, HYALURONAN, ARTHRITIS, ADHESION, RECEPTOR, CELLS, EXTRAVASATION, ANTIBODIES, ACTIVATION",
author = "Heping Xu and Ayyakkannu Manivannan and Liversidge, {Janet Mary} and Sharp, {Peter Frederick} and Forrester, {John Vincent} and Crane, {Isabel Joan}",
year = "2002",
month = "12",
language = "English",
volume = "72",
pages = "1133--1141",
journal = "Journal of Leukocyte Biology",
issn = "0741-5400",
publisher = "FASEB",
number = "6",

}

TY - JOUR

T1 - Involvement of CD44 in leukocyte trafficking at the blood-retinal barrier

AU - Xu, Heping

AU - Manivannan, Ayyakkannu

AU - Liversidge, Janet Mary

AU - Sharp, Peter Frederick

AU - Forrester, John Vincent

AU - Crane, Isabel Joan

PY - 2002/12

Y1 - 2002/12

N2 - In the present study, we investigated the involvement of CD44 in leukocyte trafficking in vivo at the blood-retinal barrier using experimental autoimmune uveoretinitis (EAU) as a model system. Leukocyte trafficking was evaluated using adoptive transfer of calcein-AM (C-AM)-labeled spleen cells harvested from syngeneic mice at pre-peak severity of EAU to mice at a similar stage of disease. CD44 and its ligand hyaluronan were upregulated in the eye during EAU. CD44-positive leukocytes were found sticking in the retinal venules and postcapillary venules but not in the retinal arterioles nor in mesenteric vessels. Preincubation of in vitro GAM-labeled leukocytes with anti-CD44 monoclonal antibodies (mAb; IM7) or high molecular weight hyaluronic acid (HA) before transfer significantly suppressed leukocyte rolling but not sticking in retinal venules and also reduced cell infiltration in the retinal parenchyma. Administration of the HA-specific enzyme hyaluronidase to mice before cell transfer also reduced leukocyte infiltration, suggesting that CD44-HA interactions are involved in leukocyte recruitment in EAU. This was further supported by the observation that disease severity was reduced by administration of anti-CD44 mAb (M) at the early leukocyte-infiltration stage. Further studies also indicated that CD44 activation was associated with increased levels of apoptosis, and this may also he in part responsible for the reduction in disease severity. These findings demonstrate that CD44 is directly involved in leukocyte-endothelial interaction in vivo and influence the trafficking of primed leukocytes to the retina and their overall survival.

AB - In the present study, we investigated the involvement of CD44 in leukocyte trafficking in vivo at the blood-retinal barrier using experimental autoimmune uveoretinitis (EAU) as a model system. Leukocyte trafficking was evaluated using adoptive transfer of calcein-AM (C-AM)-labeled spleen cells harvested from syngeneic mice at pre-peak severity of EAU to mice at a similar stage of disease. CD44 and its ligand hyaluronan were upregulated in the eye during EAU. CD44-positive leukocytes were found sticking in the retinal venules and postcapillary venules but not in the retinal arterioles nor in mesenteric vessels. Preincubation of in vitro GAM-labeled leukocytes with anti-CD44 monoclonal antibodies (mAb; IM7) or high molecular weight hyaluronic acid (HA) before transfer significantly suppressed leukocyte rolling but not sticking in retinal venules and also reduced cell infiltration in the retinal parenchyma. Administration of the HA-specific enzyme hyaluronidase to mice before cell transfer also reduced leukocyte infiltration, suggesting that CD44-HA interactions are involved in leukocyte recruitment in EAU. This was further supported by the observation that disease severity was reduced by administration of anti-CD44 mAb (M) at the early leukocyte-infiltration stage. Further studies also indicated that CD44 activation was associated with increased levels of apoptosis, and this may also he in part responsible for the reduction in disease severity. These findings demonstrate that CD44 is directly involved in leukocyte-endothelial interaction in vivo and influence the trafficking of primed leukocytes to the retina and their overall survival.

KW - adhesion molecule

KW - hyaluronic acid

KW - inflammation

KW - experimental autoimmune uveoretinitis

KW - apoptosis

KW - EXPERIMENTAL AUTOIMMUNE UVEORETINITIS

KW - ANTI-CD44 TREATMENT

KW - HYALURONAN

KW - ARTHRITIS

KW - ADHESION

KW - RECEPTOR

KW - CELLS

KW - EXTRAVASATION

KW - ANTIBODIES

KW - ACTIVATION

M3 - Article

VL - 72

SP - 1133

EP - 1141

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

IS - 6

ER -