Abstract
Siderophores are iron-chelating compounds that aid iron uptake, one of the key strategies for microorganisms to carve out ecological niches in microbially diverse environments. Desferrioxamines are the principal siderophores produced by Streptomyces spp. Their biosynthesis has been well studied and as a consequence, the chemical potential of the pathway continues to expand. With all of this in mind, our study aimed to explore extremotolerant and Lupine rhizosphere-derived Streptomyces sp. S29 for its potential antifungal capabilities. Cocultivation of isolate S29 was carried out with Aspergillus niger and Botrytis cinerea, both costly fungal phytopathogens in the wine industry. The results indicate that not only is Streptomyces sp. S29 extraordinary at producing hydroxamate siderophores but uses siderophore production as a means to ‘starve’ the fungi of iron. High resolution LC-MS/MS followed by GNPS molecular networking was used to observe the datasets for desferrioxamines and guided structure elucidation of new desferrioxamine analogues. Comparing the new chemistry, using tools like molecular networking and MS2LDA, with the known biosynthesis, we show that the chemical potential of the desferrioxamine pathway has further room for expansion.
Original language | English |
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Pages (from-to) | 95-107 |
Number of pages | 13 |
Journal | Molecular Omics |
Volume | 17 |
Issue number | 1 |
Early online date | 9 Nov 2020 |
DOIs | |
Publication status | Published - 1 Feb 2021 |
Bibliographical note
Open Access via the RSC Open Access Agreement.Acknowledgements
SAJ would like to thank the University of Aberdeen for funding doctoral studies through and Elphinstone Scholarship. DL would like to thank the Agencia Nacional de Investigación y Desarrollo (ANID) for funding doctoral studies through ‘Beca nacional de doctorado’ Scholarship. ED would like to thank the Ministry of Higher Education and Scientific Research – Sudan, together with the University of Khartoum, for joint funding of master's studies. We would like to thank to Valeria Razmilic and Jean Franco Castro for their valuable advice and work in the setup of Lupine Streptomyces culture collection. We would also like to thank the support team at GNPS and Justin J. J. van der Hooft at MS2LDA for help with data deposition and for help at any stage of running the relevant workflows.