S. pombe meiotic linear elements contain proteins related to synaptonemal complex components

Alexander Lorenz, Jennifer L. Wells, David W. Pryce, Maria Novatchkova, Frank Eisenhaber, Ramsay J. McFarlane, Josef Loidl

Research output: Contribution to journalArticle

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Abstract

The fission yeast Schizosaccharomyces pombe does not form synaptonemal complexes (SCs) in meiotic prophase nuclei. Instead, thin threads, the so-called linear elements (LEs), are observed at the corresponding stages by electron microscopy. Here, we demonstrate that S. pombe Rec10 is a protein related to the Saccharomyces cerevisiae SC protein Red1 and that it localizes to LEs. Moreover, a homologue to S. cerevisiae Hop1 does exist in S. pombe and we show by in situ immunostaining that it, and the kinase Mek1 (a homologue of which is also known to be associated with SCs), localizes to LEs. These observations indicate the evolutionary relationship of LEs with the lateral elements of SCs and suggest that these structures might exert similar functions in S. cerevisiae and S. pombe.
Original languageEnglish
Pages (from-to)3343-3351
Number of pages9
JournalJournal of Cell Science
Volume117
Issue number15
DOIs
Publication statusPublished - 1 Jul 2004

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Synaptonemal Complex
Schizosaccharomyces
Saccharomyces cerevisiae
Proteins
Prophase
Electron Microscopy
Phosphotransferases

Keywords

  • chromosome pairing
  • meiosis
  • recombination
  • yeast

Cite this

Lorenz, A., Wells, J. L., Pryce, D. W., Novatchkova, M., Eisenhaber, F., McFarlane, R. J., & Loidl, J. (2004). S. pombe meiotic linear elements contain proteins related to synaptonemal complex components. Journal of Cell Science, 117(15), 3343-3351. https://doi.org/10.1242/jcs.01203

S. pombe meiotic linear elements contain proteins related to synaptonemal complex components. / Lorenz, Alexander; Wells, Jennifer L. ; Pryce, David W.; Novatchkova, Maria; Eisenhaber, Frank; McFarlane, Ramsay J.; Loidl, Josef.

In: Journal of Cell Science, Vol. 117, No. 15, 01.07.2004, p. 3343-3351.

Research output: Contribution to journalArticle

Lorenz, A, Wells, JL, Pryce, DW, Novatchkova, M, Eisenhaber, F, McFarlane, RJ & Loidl, J 2004, 'S. pombe meiotic linear elements contain proteins related to synaptonemal complex components' Journal of Cell Science, vol. 117, no. 15, pp. 3343-3351. https://doi.org/10.1242/jcs.01203
Lorenz A, Wells JL, Pryce DW, Novatchkova M, Eisenhaber F, McFarlane RJ et al. S. pombe meiotic linear elements contain proteins related to synaptonemal complex components. Journal of Cell Science. 2004 Jul 1;117(15):3343-3351. https://doi.org/10.1242/jcs.01203
Lorenz, Alexander ; Wells, Jennifer L. ; Pryce, David W. ; Novatchkova, Maria ; Eisenhaber, Frank ; McFarlane, Ramsay J. ; Loidl, Josef. / S. pombe meiotic linear elements contain proteins related to synaptonemal complex components. In: Journal of Cell Science. 2004 ; Vol. 117, No. 15. pp. 3343-3351.
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