IS6110-mediated deletions of wild-type chromosomes of Mycobacterium tuberculosis

Z Fang, C Doig, D T Kenna, N Smittipat, P Palittapongarnpim, B Watt, K J Forbes

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The ipl locus is a site for the preferential insertion of IS6110 and has been identified as an Insertion sequence, IS1547, in its own right. Various deletions around the ipl locus of clinical isolates of Mycobacterium tuberculosis were identified, and these deletions ranged in length from several hundred base pairs up to several kilobase pairs, The most obvious feature shared by these deletions was the presence of an IS6110 copy at the deletion sites, which suggested two possible mechanisms for their occurrence, IS6110 transposition and homologous recombination, To clarify the mechanism, an investigation was conducted; the results suggest that although deletion transpositionally mediated by IS6110 was a possibility, homologous recombination was a more likely one. The implications of such chromosomal rearrangements for the evolution of M. tuberculosis, for IS6110-mediated mutagenesis, and for the development of genetic tools are discussed, The deletion of genomic DNA in isolates of M. tuberculosis has previously been noted at only a few sites. This study examined the deletional loss of genetic material at a new site and suggests that such losses may occur elsewhere too and may be more prevalent than was previously thought. Distinct from the study of laboratory-induced mutations, the detailed analysis of clinical isolates, in combination with knowledge of their evolutionary relationships to each other, gives us the opportunity to study mutational diversity in isolates that have survived in the human host and therefore offers a different perspective on the importance of particular genetic markers in pathogenesis.

Original languageEnglish
Pages (from-to)1014-1020
Number of pages7
JournalJournal of Bacteriology
Volume181
Publication statusPublished - 1999

Keywords

  • INSERTION-SEQUENCE
  • HOMOLOGOUS RECOMBINATION
  • ESCHERICHIA-COLI
  • SALMONELLA-TYPHIMURIUM
  • BOVIS BCG
  • LOCUS
  • CONSTRUCTION
  • ELEMENTS
  • MUTANT
  • IS6110

Cite this

Fang, Z., Doig, C., Kenna, D. T., Smittipat, N., Palittapongarnpim, P., Watt, B., & Forbes, K. J. (1999). IS6110-mediated deletions of wild-type chromosomes of Mycobacterium tuberculosis. Journal of Bacteriology, 181, 1014-1020.

IS6110-mediated deletions of wild-type chromosomes of Mycobacterium tuberculosis. / Fang, Z ; Doig, C ; Kenna, D T ; Smittipat, N ; Palittapongarnpim, P ; Watt, B ; Forbes, K J .

In: Journal of Bacteriology, Vol. 181, 1999, p. 1014-1020.

Research output: Contribution to journalArticle

Fang, Z, Doig, C, Kenna, DT, Smittipat, N, Palittapongarnpim, P, Watt, B & Forbes, KJ 1999, 'IS6110-mediated deletions of wild-type chromosomes of Mycobacterium tuberculosis' Journal of Bacteriology, vol. 181, pp. 1014-1020.
Fang Z, Doig C, Kenna DT, Smittipat N, Palittapongarnpim P, Watt B et al. IS6110-mediated deletions of wild-type chromosomes of Mycobacterium tuberculosis. Journal of Bacteriology. 1999;181:1014-1020.
Fang, Z ; Doig, C ; Kenna, D T ; Smittipat, N ; Palittapongarnpim, P ; Watt, B ; Forbes, K J . / IS6110-mediated deletions of wild-type chromosomes of Mycobacterium tuberculosis. In: Journal of Bacteriology. 1999 ; Vol. 181. pp. 1014-1020.
@article{fd23abfdbd8e4e699896f76da1aa56c9,
title = "IS6110-mediated deletions of wild-type chromosomes of Mycobacterium tuberculosis",
abstract = "The ipl locus is a site for the preferential insertion of IS6110 and has been identified as an Insertion sequence, IS1547, in its own right. Various deletions around the ipl locus of clinical isolates of Mycobacterium tuberculosis were identified, and these deletions ranged in length from several hundred base pairs up to several kilobase pairs, The most obvious feature shared by these deletions was the presence of an IS6110 copy at the deletion sites, which suggested two possible mechanisms for their occurrence, IS6110 transposition and homologous recombination, To clarify the mechanism, an investigation was conducted; the results suggest that although deletion transpositionally mediated by IS6110 was a possibility, homologous recombination was a more likely one. The implications of such chromosomal rearrangements for the evolution of M. tuberculosis, for IS6110-mediated mutagenesis, and for the development of genetic tools are discussed, The deletion of genomic DNA in isolates of M. tuberculosis has previously been noted at only a few sites. This study examined the deletional loss of genetic material at a new site and suggests that such losses may occur elsewhere too and may be more prevalent than was previously thought. Distinct from the study of laboratory-induced mutations, the detailed analysis of clinical isolates, in combination with knowledge of their evolutionary relationships to each other, gives us the opportunity to study mutational diversity in isolates that have survived in the human host and therefore offers a different perspective on the importance of particular genetic markers in pathogenesis.",
keywords = "INSERTION-SEQUENCE, HOMOLOGOUS RECOMBINATION, ESCHERICHIA-COLI, SALMONELLA-TYPHIMURIUM, BOVIS BCG, LOCUS, CONSTRUCTION, ELEMENTS, MUTANT, IS6110",
author = "Z Fang and C Doig and Kenna, {D T} and N Smittipat and P Palittapongarnpim and B Watt and Forbes, {K J}",
year = "1999",
language = "English",
volume = "181",
pages = "1014--1020",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",

}

TY - JOUR

T1 - IS6110-mediated deletions of wild-type chromosomes of Mycobacterium tuberculosis

AU - Fang, Z

AU - Doig, C

AU - Kenna, D T

AU - Smittipat, N

AU - Palittapongarnpim, P

AU - Watt, B

AU - Forbes, K J

PY - 1999

Y1 - 1999

N2 - The ipl locus is a site for the preferential insertion of IS6110 and has been identified as an Insertion sequence, IS1547, in its own right. Various deletions around the ipl locus of clinical isolates of Mycobacterium tuberculosis were identified, and these deletions ranged in length from several hundred base pairs up to several kilobase pairs, The most obvious feature shared by these deletions was the presence of an IS6110 copy at the deletion sites, which suggested two possible mechanisms for their occurrence, IS6110 transposition and homologous recombination, To clarify the mechanism, an investigation was conducted; the results suggest that although deletion transpositionally mediated by IS6110 was a possibility, homologous recombination was a more likely one. The implications of such chromosomal rearrangements for the evolution of M. tuberculosis, for IS6110-mediated mutagenesis, and for the development of genetic tools are discussed, The deletion of genomic DNA in isolates of M. tuberculosis has previously been noted at only a few sites. This study examined the deletional loss of genetic material at a new site and suggests that such losses may occur elsewhere too and may be more prevalent than was previously thought. Distinct from the study of laboratory-induced mutations, the detailed analysis of clinical isolates, in combination with knowledge of their evolutionary relationships to each other, gives us the opportunity to study mutational diversity in isolates that have survived in the human host and therefore offers a different perspective on the importance of particular genetic markers in pathogenesis.

AB - The ipl locus is a site for the preferential insertion of IS6110 and has been identified as an Insertion sequence, IS1547, in its own right. Various deletions around the ipl locus of clinical isolates of Mycobacterium tuberculosis were identified, and these deletions ranged in length from several hundred base pairs up to several kilobase pairs, The most obvious feature shared by these deletions was the presence of an IS6110 copy at the deletion sites, which suggested two possible mechanisms for their occurrence, IS6110 transposition and homologous recombination, To clarify the mechanism, an investigation was conducted; the results suggest that although deletion transpositionally mediated by IS6110 was a possibility, homologous recombination was a more likely one. The implications of such chromosomal rearrangements for the evolution of M. tuberculosis, for IS6110-mediated mutagenesis, and for the development of genetic tools are discussed, The deletion of genomic DNA in isolates of M. tuberculosis has previously been noted at only a few sites. This study examined the deletional loss of genetic material at a new site and suggests that such losses may occur elsewhere too and may be more prevalent than was previously thought. Distinct from the study of laboratory-induced mutations, the detailed analysis of clinical isolates, in combination with knowledge of their evolutionary relationships to each other, gives us the opportunity to study mutational diversity in isolates that have survived in the human host and therefore offers a different perspective on the importance of particular genetic markers in pathogenesis.

KW - INSERTION-SEQUENCE

KW - HOMOLOGOUS RECOMBINATION

KW - ESCHERICHIA-COLI

KW - SALMONELLA-TYPHIMURIUM

KW - BOVIS BCG

KW - LOCUS

KW - CONSTRUCTION

KW - ELEMENTS

KW - MUTANT

KW - IS6110

M3 - Article

VL - 181

SP - 1014

EP - 1020

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

ER -