Isolation and characterization of a mutant Chinese hamster ovary cell line that is resistant to Chlamydia trachomatis infection at a novel step in the attachment process

R A Carabeo, T Hackstadt

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41 Citations (Scopus)

Abstract

Host factors involved in Chlamydia trachomatis pathogenesis were investigated by random chemical mutagenesis of Chinese hamster ovary (CHO-K1) cells followed by selection for clones resistant to chlamydial infection. A clonal mutant cell line, D4.1-3, refractory to infection by the C. trachomatis L2 serovar was isolated. The D4.1-3 cell line appears to be lacking in a previously undescribed temperature-dependent and heparin-resistant binding step that occurs subsequent to engagement of cell surface heparan sulfate by L2 elementary bodies. This novel binding step differentiates the lymphogranuloma venereum (LGV) serovar from other serovars and may contribute the different pathologies associated with LGV and non-LGV strains.
Original languageEnglish
Pages (from-to)5899-904
Number of pages6
JournalInfection and Immunity
Volume69
Issue number9
Publication statusPublished - Sep 2001

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Chlamydia Infections
Chlamydia trachomatis
Cricetulus
Lymphogranuloma Venereum
Ovary
Cell Line
Heparitin Sulfate
CHO Cells
Infection
Mutagenesis
Heparin
Clone Cells
Pathology
Temperature
Serogroup

Keywords

  • Ethyl Methanesulfonate
  • Animals
  • Heparitin Sulfate
  • Humans
  • Temperature
  • Chlamydia trachomatis
  • CHO Cells
  • Bacterial Adhesion
  • Cell Line
  • Mutagenesis
  • Cricetinae

Cite this

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title = "Isolation and characterization of a mutant Chinese hamster ovary cell line that is resistant to Chlamydia trachomatis infection at a novel step in the attachment process",
abstract = "Host factors involved in Chlamydia trachomatis pathogenesis were investigated by random chemical mutagenesis of Chinese hamster ovary (CHO-K1) cells followed by selection for clones resistant to chlamydial infection. A clonal mutant cell line, D4.1-3, refractory to infection by the C. trachomatis L2 serovar was isolated. The D4.1-3 cell line appears to be lacking in a previously undescribed temperature-dependent and heparin-resistant binding step that occurs subsequent to engagement of cell surface heparan sulfate by L2 elementary bodies. This novel binding step differentiates the lymphogranuloma venereum (LGV) serovar from other serovars and may contribute the different pathologies associated with LGV and non-LGV strains.",
keywords = "Ethyl Methanesulfonate, Animals, Heparitin Sulfate, Humans, Temperature, Chlamydia trachomatis, CHO Cells, Bacterial Adhesion, Cell Line, Mutagenesis, Cricetinae",
author = "Carabeo, {R A} and T Hackstadt",
year = "2001",
month = "9",
language = "English",
volume = "69",
pages = "5899--904",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "9",

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TY - JOUR

T1 - Isolation and characterization of a mutant Chinese hamster ovary cell line that is resistant to Chlamydia trachomatis infection at a novel step in the attachment process

AU - Carabeo, R A

AU - Hackstadt, T

PY - 2001/9

Y1 - 2001/9

N2 - Host factors involved in Chlamydia trachomatis pathogenesis were investigated by random chemical mutagenesis of Chinese hamster ovary (CHO-K1) cells followed by selection for clones resistant to chlamydial infection. A clonal mutant cell line, D4.1-3, refractory to infection by the C. trachomatis L2 serovar was isolated. The D4.1-3 cell line appears to be lacking in a previously undescribed temperature-dependent and heparin-resistant binding step that occurs subsequent to engagement of cell surface heparan sulfate by L2 elementary bodies. This novel binding step differentiates the lymphogranuloma venereum (LGV) serovar from other serovars and may contribute the different pathologies associated with LGV and non-LGV strains.

AB - Host factors involved in Chlamydia trachomatis pathogenesis were investigated by random chemical mutagenesis of Chinese hamster ovary (CHO-K1) cells followed by selection for clones resistant to chlamydial infection. A clonal mutant cell line, D4.1-3, refractory to infection by the C. trachomatis L2 serovar was isolated. The D4.1-3 cell line appears to be lacking in a previously undescribed temperature-dependent and heparin-resistant binding step that occurs subsequent to engagement of cell surface heparan sulfate by L2 elementary bodies. This novel binding step differentiates the lymphogranuloma venereum (LGV) serovar from other serovars and may contribute the different pathologies associated with LGV and non-LGV strains.

KW - Ethyl Methanesulfonate

KW - Animals

KW - Heparitin Sulfate

KW - Humans

KW - Temperature

KW - Chlamydia trachomatis

KW - CHO Cells

KW - Bacterial Adhesion

KW - Cell Line

KW - Mutagenesis

KW - Cricetinae

M3 - Article

VL - 69

SP - 5899

EP - 5904

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 9

ER -