Isolation and characterization of a mutant Chinese hamster ovary cell line that is resistant to Chlamydia trachomatis infection at a novel step in the attachment process

R A Carabeo, T Hackstadt

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Host factors involved in Chlamydia trachomatis pathogenesis were investigated by random chemical mutagenesis of Chinese hamster ovary (CHO-K1) cells followed by selection for clones resistant to chlamydial infection. A clonal mutant cell line, D4.1-3, refractory to infection by the C. trachomatis L2 serovar was isolated. The D4.1-3 cell line appears to be lacking in a previously undescribed temperature-dependent and heparin-resistant binding step that occurs subsequent to engagement of cell surface heparan sulfate by L2 elementary bodies. This novel binding step differentiates the lymphogranuloma venereum (LGV) serovar from other serovars and may contribute the different pathologies associated with LGV and non-LGV strains.
Original languageEnglish
Pages (from-to)5899-904
Number of pages6
JournalInfection and Immunity
Volume69
Issue number9
Publication statusPublished - Sep 2001

    Fingerprint

Keywords

  • Ethyl Methanesulfonate
  • Animals
  • Heparitin Sulfate
  • Humans
  • Temperature
  • Chlamydia trachomatis
  • CHO Cells
  • Bacterial Adhesion
  • Cell Line
  • Mutagenesis
  • Cricetinae

Cite this