Isolation and characterization of CXC receptor genes in a range of elasmobranchs

A Goostrey, G Jones, C J Secombes

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The CXC group of chemokines exert their cellular effects via the CXCR group of G-protein coupled receptors. Six CXCR genes have been identified in humans (CXCR1-6), and homologues to some of these have been isolated from a range of vertebrate species. Here we isolate and characterize CXCR genes from a range of elasmobranch species. One CXCR 1/2 gene fragment isolated from Scyliorhinus caniculus (lesser spotted catshark), and two CXCR1/2 copies from each of the elasmobranchs, Cetorhinus maximus (basking shark), Carcharodon carcharias (great white shark). and Raja naevus (cuckoo ray), exhibit high similarity to both CXCR1 and CXCR2. The two copies evident in the cuckoo ray and lamniform sharks provide strong evidence of CXCR1/2 lineage specific duplication in rays and sharks. A CXCR fragment isolated front Lamna ditropis (salmon shark) shows high similarity to a range of CXCR4 genes and strong clustering with CXCR4 gene homologues was apparent during phylogenetic reconstruction. (C) 2004 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)229-242
Number of pages14
JournalDevelopmental and Comparative Immunology
Volume29
DOIs
Publication statusPublished - 2005

Keywords

  • chemokine receptor
  • evolution
  • immune system
  • sharks
  • rays
  • TROUT ONCORHYNCHUS-MYKISS
  • CHEMOKINE RECEPTORS
  • MOLECULAR-CLONING
  • CC-CHEMOKINE
  • RHEUMATOID-ARTHRITIS
  • INTERLEUKIN-8 CDNA
  • DNA VACCINATION
  • SEQUENCE
  • IDENTIFICATION
  • CYTOKINE

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