Isolation and characterization of CXC receptor genes in a range of elasmobranchs

A Goostrey, G Jones, C J Secombes

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The CXC group of chemokines exert their cellular effects via the CXCR group of G-protein coupled receptors. Six CXCR genes have been identified in humans (CXCR1-6), and homologues to some of these have been isolated from a range of vertebrate species. Here we isolate and characterize CXCR genes from a range of elasmobranch species. One CXCR 1/2 gene fragment isolated from Scyliorhinus caniculus (lesser spotted catshark), and two CXCR1/2 copies from each of the elasmobranchs, Cetorhinus maximus (basking shark), Carcharodon carcharias (great white shark). and Raja naevus (cuckoo ray), exhibit high similarity to both CXCR1 and CXCR2. The two copies evident in the cuckoo ray and lamniform sharks provide strong evidence of CXCR1/2 lineage specific duplication in rays and sharks. A CXCR fragment isolated front Lamna ditropis (salmon shark) shows high similarity to a range of CXCR4 genes and strong clustering with CXCR4 gene homologues was apparent during phylogenetic reconstruction. (C) 2004 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)229-242
Number of pages14
JournalDevelopmental and Comparative Immunology
Volume29
DOIs
Publication statusPublished - 2005

Keywords

  • chemokine receptor
  • evolution
  • immune system
  • sharks
  • rays
  • TROUT ONCORHYNCHUS-MYKISS
  • CHEMOKINE RECEPTORS
  • MOLECULAR-CLONING
  • CC-CHEMOKINE
  • RHEUMATOID-ARTHRITIS
  • INTERLEUKIN-8 CDNA
  • DNA VACCINATION
  • SEQUENCE
  • IDENTIFICATION
  • CYTOKINE

Cite this

Isolation and characterization of CXC receptor genes in a range of elasmobranchs. / Goostrey, A ; Jones, G ; Secombes, C J .

In: Developmental and Comparative Immunology, Vol. 29, 2005, p. 229-242.

Research output: Contribution to journalArticle

@article{6553ec3ad990483e9e741477dec41b9c,
title = "Isolation and characterization of CXC receptor genes in a range of elasmobranchs",
abstract = "The CXC group of chemokines exert their cellular effects via the CXCR group of G-protein coupled receptors. Six CXCR genes have been identified in humans (CXCR1-6), and homologues to some of these have been isolated from a range of vertebrate species. Here we isolate and characterize CXCR genes from a range of elasmobranch species. One CXCR 1/2 gene fragment isolated from Scyliorhinus caniculus (lesser spotted catshark), and two CXCR1/2 copies from each of the elasmobranchs, Cetorhinus maximus (basking shark), Carcharodon carcharias (great white shark). and Raja naevus (cuckoo ray), exhibit high similarity to both CXCR1 and CXCR2. The two copies evident in the cuckoo ray and lamniform sharks provide strong evidence of CXCR1/2 lineage specific duplication in rays and sharks. A CXCR fragment isolated front Lamna ditropis (salmon shark) shows high similarity to a range of CXCR4 genes and strong clustering with CXCR4 gene homologues was apparent during phylogenetic reconstruction. (C) 2004 Elsevier Ltd. All rights reserved.",
keywords = "chemokine receptor, evolution, immune system, sharks, rays, TROUT ONCORHYNCHUS-MYKISS, CHEMOKINE RECEPTORS, MOLECULAR-CLONING, CC-CHEMOKINE, RHEUMATOID-ARTHRITIS, INTERLEUKIN-8 CDNA, DNA VACCINATION, SEQUENCE, IDENTIFICATION, CYTOKINE",
author = "A Goostrey and G Jones and Secombes, {C J}",
year = "2005",
doi = "10.1016/j.dci.2004.06.012",
language = "English",
volume = "29",
pages = "229--242",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Isolation and characterization of CXC receptor genes in a range of elasmobranchs

AU - Goostrey, A

AU - Jones, G

AU - Secombes, C J

PY - 2005

Y1 - 2005

N2 - The CXC group of chemokines exert their cellular effects via the CXCR group of G-protein coupled receptors. Six CXCR genes have been identified in humans (CXCR1-6), and homologues to some of these have been isolated from a range of vertebrate species. Here we isolate and characterize CXCR genes from a range of elasmobranch species. One CXCR 1/2 gene fragment isolated from Scyliorhinus caniculus (lesser spotted catshark), and two CXCR1/2 copies from each of the elasmobranchs, Cetorhinus maximus (basking shark), Carcharodon carcharias (great white shark). and Raja naevus (cuckoo ray), exhibit high similarity to both CXCR1 and CXCR2. The two copies evident in the cuckoo ray and lamniform sharks provide strong evidence of CXCR1/2 lineage specific duplication in rays and sharks. A CXCR fragment isolated front Lamna ditropis (salmon shark) shows high similarity to a range of CXCR4 genes and strong clustering with CXCR4 gene homologues was apparent during phylogenetic reconstruction. (C) 2004 Elsevier Ltd. All rights reserved.

AB - The CXC group of chemokines exert their cellular effects via the CXCR group of G-protein coupled receptors. Six CXCR genes have been identified in humans (CXCR1-6), and homologues to some of these have been isolated from a range of vertebrate species. Here we isolate and characterize CXCR genes from a range of elasmobranch species. One CXCR 1/2 gene fragment isolated from Scyliorhinus caniculus (lesser spotted catshark), and two CXCR1/2 copies from each of the elasmobranchs, Cetorhinus maximus (basking shark), Carcharodon carcharias (great white shark). and Raja naevus (cuckoo ray), exhibit high similarity to both CXCR1 and CXCR2. The two copies evident in the cuckoo ray and lamniform sharks provide strong evidence of CXCR1/2 lineage specific duplication in rays and sharks. A CXCR fragment isolated front Lamna ditropis (salmon shark) shows high similarity to a range of CXCR4 genes and strong clustering with CXCR4 gene homologues was apparent during phylogenetic reconstruction. (C) 2004 Elsevier Ltd. All rights reserved.

KW - chemokine receptor

KW - evolution

KW - immune system

KW - sharks

KW - rays

KW - TROUT ONCORHYNCHUS-MYKISS

KW - CHEMOKINE RECEPTORS

KW - MOLECULAR-CLONING

KW - CC-CHEMOKINE

KW - RHEUMATOID-ARTHRITIS

KW - INTERLEUKIN-8 CDNA

KW - DNA VACCINATION

KW - SEQUENCE

KW - IDENTIFICATION

KW - CYTOKINE

U2 - 10.1016/j.dci.2004.06.012

DO - 10.1016/j.dci.2004.06.012

M3 - Article

VL - 29

SP - 229

EP - 242

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

ER -