Isolation and partial characterization of a lead-accumulating Brassica juncea mutant

R N Schulman, D E Salt, I Raskin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A new screening method for non-destructive, high-sensitivity, high-throughput isolation of plant mutants capable of accumulating large amounts of heavy metals has been developed. This method is based on incubating seedlings in a solution containing radioisotopes of the metals of interest and visualizing the tissue accumulation of these metals with a phosphorimager. We used this technique to isolate mutants of Brassica juncea (L.) Czern with increased accumulation of Cd and Pb for use in phytoremediation, an emerging technology using plants to remediate polluted soil and water. Approximately 50,000 M2 seedlings were screened and 21 mutants were recovered that retained increased accumulation through the third generation. Mutant 7/15_1 is characterized by enhanced Pb accumulation per unit of root fresh weight, stunted root growth, and decreased root cell size. Data indicate that roots of 7/15-1 contain more cell-wall material on a fresh-weight basis than roots of the wild-type, which may at least partially explain its ability to accumulate more Pb.

Original languageEnglish
Pages (from-to)398-404
Number of pages7
JournalTheoretical and Applied Genetics
Volume99
Issue number3-4
Publication statusPublished - Aug 1999

Keywords

  • Brassica juncea
  • screening
  • mutants
  • cadmium
  • lead
  • ARABIDOPSIS ROOTS
  • INDIAN MUSTARD
  • HEAVY-METALS
  • PHYTOREMEDIATION
  • PLANTS
  • ENVIRONMENT
  • INHIBITORS
  • ETHYLENE
  • GROWTH

Cite this

Isolation and partial characterization of a lead-accumulating Brassica juncea mutant. / Schulman, R N ; Salt, D E ; Raskin, I .

In: Theoretical and Applied Genetics, Vol. 99, No. 3-4, 08.1999, p. 398-404.

Research output: Contribution to journalArticle

@article{e168c90007d642a999d252125a184960,
title = "Isolation and partial characterization of a lead-accumulating Brassica juncea mutant",
abstract = "A new screening method for non-destructive, high-sensitivity, high-throughput isolation of plant mutants capable of accumulating large amounts of heavy metals has been developed. This method is based on incubating seedlings in a solution containing radioisotopes of the metals of interest and visualizing the tissue accumulation of these metals with a phosphorimager. We used this technique to isolate mutants of Brassica juncea (L.) Czern with increased accumulation of Cd and Pb for use in phytoremediation, an emerging technology using plants to remediate polluted soil and water. Approximately 50,000 M2 seedlings were screened and 21 mutants were recovered that retained increased accumulation through the third generation. Mutant 7/15_1 is characterized by enhanced Pb accumulation per unit of root fresh weight, stunted root growth, and decreased root cell size. Data indicate that roots of 7/15-1 contain more cell-wall material on a fresh-weight basis than roots of the wild-type, which may at least partially explain its ability to accumulate more Pb.",
keywords = "Brassica juncea, screening, mutants, cadmium, lead, ARABIDOPSIS ROOTS, INDIAN MUSTARD, HEAVY-METALS, PHYTOREMEDIATION, PLANTS, ENVIRONMENT, INHIBITORS, ETHYLENE, GROWTH",
author = "Schulman, {R N} and Salt, {D E} and I Raskin",
year = "1999",
month = "8",
language = "English",
volume = "99",
pages = "398--404",
journal = "Theoretical and Applied Genetics",
issn = "0040-5752",
number = "3-4",

}

TY - JOUR

T1 - Isolation and partial characterization of a lead-accumulating Brassica juncea mutant

AU - Schulman, R N

AU - Salt, D E

AU - Raskin, I

PY - 1999/8

Y1 - 1999/8

N2 - A new screening method for non-destructive, high-sensitivity, high-throughput isolation of plant mutants capable of accumulating large amounts of heavy metals has been developed. This method is based on incubating seedlings in a solution containing radioisotopes of the metals of interest and visualizing the tissue accumulation of these metals with a phosphorimager. We used this technique to isolate mutants of Brassica juncea (L.) Czern with increased accumulation of Cd and Pb for use in phytoremediation, an emerging technology using plants to remediate polluted soil and water. Approximately 50,000 M2 seedlings were screened and 21 mutants were recovered that retained increased accumulation through the third generation. Mutant 7/15_1 is characterized by enhanced Pb accumulation per unit of root fresh weight, stunted root growth, and decreased root cell size. Data indicate that roots of 7/15-1 contain more cell-wall material on a fresh-weight basis than roots of the wild-type, which may at least partially explain its ability to accumulate more Pb.

AB - A new screening method for non-destructive, high-sensitivity, high-throughput isolation of plant mutants capable of accumulating large amounts of heavy metals has been developed. This method is based on incubating seedlings in a solution containing radioisotopes of the metals of interest and visualizing the tissue accumulation of these metals with a phosphorimager. We used this technique to isolate mutants of Brassica juncea (L.) Czern with increased accumulation of Cd and Pb for use in phytoremediation, an emerging technology using plants to remediate polluted soil and water. Approximately 50,000 M2 seedlings were screened and 21 mutants were recovered that retained increased accumulation through the third generation. Mutant 7/15_1 is characterized by enhanced Pb accumulation per unit of root fresh weight, stunted root growth, and decreased root cell size. Data indicate that roots of 7/15-1 contain more cell-wall material on a fresh-weight basis than roots of the wild-type, which may at least partially explain its ability to accumulate more Pb.

KW - Brassica juncea

KW - screening

KW - mutants

KW - cadmium

KW - lead

KW - ARABIDOPSIS ROOTS

KW - INDIAN MUSTARD

KW - HEAVY-METALS

KW - PHYTOREMEDIATION

KW - PLANTS

KW - ENVIRONMENT

KW - INHIBITORS

KW - ETHYLENE

KW - GROWTH

M3 - Article

VL - 99

SP - 398

EP - 404

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

SN - 0040-5752

IS - 3-4

ER -