Isolation and recombinant expression of an MHV-JHM neutralising monoclonal antibody

Andreas Kolb, M Lechermaier, A Heister, A Toksoy, S G Siddell

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    The monoclonal antibody Al (mab Al) efficiently neutralises the infection of susceptible cells by the murine hepatitis virus MHV-JHM in vitro and in vivo (Wege et al., 1984). The variable regions of mab Al were amplified from mRNA of the respective hybridoma cell line by RT-PCR and integrated into different eukaryotic expression vectors. The biological function of the recombinant antibody constructs was verified by virus neutralisation assays. Whereas a complete recombinant antibody (mab Alrec.) expressed in transfected murine myeloma cells inhibited the MHV-JHM infection as well as the parental antibody, a single-chain Fv derived from mab Al did not show any neutralising activity.

    Original languageEnglish
    Pages (from-to)657-664
    Number of pages8
    JournalAdvances in Experimental Medicine and Biology
    Volume440
    Publication statusPublished - 1998

    Keywords

    • protein

    Cite this

    Isolation and recombinant expression of an MHV-JHM neutralising monoclonal antibody. / Kolb, Andreas; Lechermaier, M ; Heister, A ; Toksoy, A ; Siddell, S G .

    In: Advances in Experimental Medicine and Biology, Vol. 440, 1998, p. 657-664.

    Research output: Contribution to journalArticle

    Kolb, Andreas ; Lechermaier, M ; Heister, A ; Toksoy, A ; Siddell, S G . / Isolation and recombinant expression of an MHV-JHM neutralising monoclonal antibody. In: Advances in Experimental Medicine and Biology. 1998 ; Vol. 440. pp. 657-664.
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    abstract = "The monoclonal antibody Al (mab Al) efficiently neutralises the infection of susceptible cells by the murine hepatitis virus MHV-JHM in vitro and in vivo (Wege et al., 1984). The variable regions of mab Al were amplified from mRNA of the respective hybridoma cell line by RT-PCR and integrated into different eukaryotic expression vectors. The biological function of the recombinant antibody constructs was verified by virus neutralisation assays. Whereas a complete recombinant antibody (mab Alrec.) expressed in transfected murine myeloma cells inhibited the MHV-JHM infection as well as the parental antibody, a single-chain Fv derived from mab Al did not show any neutralising activity.",
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    AU - Lechermaier, M

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    AU - Siddell, S G

    PY - 1998

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    AB - The monoclonal antibody Al (mab Al) efficiently neutralises the infection of susceptible cells by the murine hepatitis virus MHV-JHM in vitro and in vivo (Wege et al., 1984). The variable regions of mab Al were amplified from mRNA of the respective hybridoma cell line by RT-PCR and integrated into different eukaryotic expression vectors. The biological function of the recombinant antibody constructs was verified by virus neutralisation assays. Whereas a complete recombinant antibody (mab Alrec.) expressed in transfected murine myeloma cells inhibited the MHV-JHM infection as well as the parental antibody, a single-chain Fv derived from mab Al did not show any neutralising activity.

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