Isolation and recombinant expression of an MHV-JHM neutralising monoclonal antibody

Andreas Kolb, M Lechermaier, A Heister, A Toksoy, S G Siddell

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    Abstract

    The monoclonal antibody Al (mab Al) efficiently neutralises the infection of susceptible cells by the murine hepatitis virus MHV-JHM in vitro and in vivo (Wege et al., 1984). The variable regions of mab Al were amplified from mRNA of the respective hybridoma cell line by RT-PCR and integrated into different eukaryotic expression vectors. The biological function of the recombinant antibody constructs was verified by virus neutralisation assays. Whereas a complete recombinant antibody (mab Alrec.) expressed in transfected murine myeloma cells inhibited the MHV-JHM infection as well as the parental antibody, a single-chain Fv derived from mab Al did not show any neutralising activity.

    Original languageEnglish
    Pages (from-to)657-664
    Number of pages8
    JournalAdvances in Experimental Medicine and Biology
    Volume440
    Publication statusPublished - 1998

    Keywords

    • protein

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