Abstract
Arachidonylethanolamide, an arachidonic acid derivative in porcine brain, was identified in a screen for endogenous ligands for the cannabinoid receptor. The structure of this compound, which has been named "anandamide," was determined by mass spectrometry and nuclear magnetic resonance spectroscopy and was confirmed by synthesis. Anandamide inhibited the specific binding of a radiolabeled cannabinoid probe to synaptosomal membranes in a manner typical of competitive ligands and produced a concentration-dependent inhibition of the electrically evoked twitch response of the mouse vas deferens, a characteristic effect of psychotropic cannabinoids. These properties suggest that anandamide may function as a natural ligand for the cannabinoid receptor.
Original language | English |
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Pages (from-to) | 1946-1949 |
Number of pages | 4 |
Journal | Science |
Volume | 258 |
Issue number | 5090 |
Publication status | Published - 18 Dec 1992 |
Keywords
- RAT-BRAIN
- LOCALIZATION
- rat-brain
- localization
Cite this
Isolation and structure of a brain constituent that binds to the cannabinoid receptor. / Devane, W A ; Hanus, L ; Breuer, A ; Pertwee, Roger Guy; Stevenson, Lesley Ann; Griffin, G ; Gibson, D; Mandelbaum, A ; Etinger, A ; Mechoulam, R .
In: Science, Vol. 258, No. 5090, 18.12.1992, p. 1946-1949.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Isolation and structure of a brain constituent that binds to the cannabinoid receptor
AU - Devane, W A
AU - Hanus, L
AU - Breuer, A
AU - Pertwee, Roger Guy
AU - Stevenson, Lesley Ann
AU - Griffin, G
AU - Gibson, D
AU - Mandelbaum, A
AU - Etinger, A
AU - Mechoulam, R
PY - 1992/12/18
Y1 - 1992/12/18
N2 - Arachidonylethanolamide, an arachidonic acid derivative in porcine brain, was identified in a screen for endogenous ligands for the cannabinoid receptor. The structure of this compound, which has been named "anandamide," was determined by mass spectrometry and nuclear magnetic resonance spectroscopy and was confirmed by synthesis. Anandamide inhibited the specific binding of a radiolabeled cannabinoid probe to synaptosomal membranes in a manner typical of competitive ligands and produced a concentration-dependent inhibition of the electrically evoked twitch response of the mouse vas deferens, a characteristic effect of psychotropic cannabinoids. These properties suggest that anandamide may function as a natural ligand for the cannabinoid receptor.
AB - Arachidonylethanolamide, an arachidonic acid derivative in porcine brain, was identified in a screen for endogenous ligands for the cannabinoid receptor. The structure of this compound, which has been named "anandamide," was determined by mass spectrometry and nuclear magnetic resonance spectroscopy and was confirmed by synthesis. Anandamide inhibited the specific binding of a radiolabeled cannabinoid probe to synaptosomal membranes in a manner typical of competitive ligands and produced a concentration-dependent inhibition of the electrically evoked twitch response of the mouse vas deferens, a characteristic effect of psychotropic cannabinoids. These properties suggest that anandamide may function as a natural ligand for the cannabinoid receptor.
KW - RAT-BRAIN
KW - LOCALIZATION
KW - rat-brain
KW - localization
M3 - Article
VL - 258
SP - 1946
EP - 1949
JO - Science
JF - Science
SN - 0036-8075
IS - 5090
ER -