Isolation of a novel fish thymidylate kinase gene, upregulated in Atlantic salmon (Salmo salar L.) following infection with the monogenean parasite Gyrodactylus salaris

Catherine A. Collins, Kjetil Olstad, Erik Sterud, Catherine S. Jones, Leslie R. Noble, Tor Atle Mo, Carey O. Cunningham

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Analysis of differential gene expression in salmon (Salmo salar) blood following infection with the monogenean parasite Gyrodactylus salaris, resulted in the isolation of a thymidylate kinase gene not previously described from fish and which showed similarity to an LPS-inducible thymidylate kinase gene isolated from mouse macrophages. This salmon TYKi-like gene may play a role in an innate generalised response to pathogen infection as it was upregulated in salmon following infection with the parasite, and also in response to injection with the immunostimulants LPS and Poly I:C, used to emulate bacterial and viral infections, respectively. The possible role of this gene in the biosynthesis of mitochondrial DNA in activated macrophages, in response to G. salaris infection is discussed. (c) 2007 Published by Elsevier Ltd.

Original languageEnglish
Pages (from-to)793-807
Number of pages15
JournalFish & Shellfish Immunology
Volume23
Issue number4
DOIs
Publication statusPublished - Oct 2007

Keywords

  • salmon
  • Gyrodactylus
  • infection
  • differential display
  • gene expression
  • immune response
  • thymidylate kinase
  • RT-PCR
  • rainbow-trout skin
  • messenger-RNA
  • Malmberg platyhelminthes
  • host-specificity
  • expression
  • susceptibility
  • Derjavini
  • stocks
  • resistant

Cite this

Isolation of a novel fish thymidylate kinase gene, upregulated in Atlantic salmon (Salmo salar L.) following infection with the monogenean parasite Gyrodactylus salaris. / Collins, Catherine A.; Olstad, Kjetil; Sterud, Erik; Jones, Catherine S.; Noble, Leslie R.; Mo, Tor Atle; Cunningham, Carey O.

In: Fish & Shellfish Immunology, Vol. 23, No. 4, 10.2007, p. 793-807.

Research output: Contribution to journalArticle

Collins, Catherine A. ; Olstad, Kjetil ; Sterud, Erik ; Jones, Catherine S. ; Noble, Leslie R. ; Mo, Tor Atle ; Cunningham, Carey O. / Isolation of a novel fish thymidylate kinase gene, upregulated in Atlantic salmon (Salmo salar L.) following infection with the monogenean parasite Gyrodactylus salaris. In: Fish & Shellfish Immunology. 2007 ; Vol. 23, No. 4. pp. 793-807.
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abstract = "Analysis of differential gene expression in salmon (Salmo salar) blood following infection with the monogenean parasite Gyrodactylus salaris, resulted in the isolation of a thymidylate kinase gene not previously described from fish and which showed similarity to an LPS-inducible thymidylate kinase gene isolated from mouse macrophages. This salmon TYKi-like gene may play a role in an innate generalised response to pathogen infection as it was upregulated in salmon following infection with the parasite, and also in response to injection with the immunostimulants LPS and Poly I:C, used to emulate bacterial and viral infections, respectively. The possible role of this gene in the biosynthesis of mitochondrial DNA in activated macrophages, in response to G. salaris infection is discussed. (c) 2007 Published by Elsevier Ltd.",
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