Isolation of microsatellite loci in the freshwater fish, the bitterling Rhodeus sericeus (Teleostei: Cyprinidae)

D Dawson, Tamsin Burland, Alex Douglas, Steven LeComber, M Bradshaw

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Abstract

We isolated 30 microsatellites from the freshwater fish, the bitterling Rhodeus sericeus.
Twenty-three microsatellite sequences possessed sufficient flanking DNA from which to
design primers. Twelve loci were characterized and all were found to be polymorphic.
These loci were isolated and characterized as part of a project to investigate the consequences
of male alternative mating tactics and sperm competition using the bitterling as a
model species.
Original languageEnglish
Pages (from-to)199-202
Number of pages4
JournalMolecular Ecology
Volume3
Issue number2
Early online date21 Feb 2003
DOIs
Publication statusPublished - 2003

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