Isolation of microsatellite primers for Melampyrum sylvaticum (Orobanchaceae), an endangered plant in the United Kingdom

Rhiannon J. Crichton, Jane Squirrell, Sarah J. Woodin, Sarah E. Dalrymple, Peter M. Hollingsworth

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

• Premise of the study: Microsatellite markers were developed for the hemiparasitic plant Melampyrum sylvaticum to investigate the breeding system, genetic diversity, and structure of populations in the United Kingdom, Sweden, and Norway. • Methods and Results: Microsatellites were isolated from genomic DNA using an enrichment protocol. Twenty-nine loci were characterized in two individuals from each of 15 geographically disparate populations ("global"). Seven polymorphic loci were further characterized in one population ("local"). The number of alleles per locus ranged from two to 12 in the global sample and one to seven in the local sample. The expected heterozygosity ranged from 0-0.75, the observed heterozygosity from 0-0.1, and the inbreeding coefficient from 0.84-1 in the local sample. • Conclusions: The results show the utility of these novel polymorphic microsatellite markers for further conservation genetic analyses. The strong deficit of heterozygosity across all loci in the local sample suggests the species may be inbreeding.

Original languageEnglish
Pages (from-to)e457-e459
Number of pages3
JournalAmerican Journal of Botany
Volume99
Issue number11
Early online date1 Nov 2012
DOIs
Publication statusPublished - 19 Nov 2012

Fingerprint

Orobanchaceae
Melampyrum
heterozygosity
endangered species
Microsatellite Repeats
United Kingdom
Inbreeding
microsatellite repeats
inbreeding
loci
Population
conservation genetics
Genetic Structures
Norway
Sweden
sampling
reproductive strategy
genetic structure
Breeding
inbreeding coefficient

Keywords

  • Conservation
  • Hemiparasite
  • Inbreeding
  • Melampyrum sylvaticum
  • Microsatellite
  • Orobanchaceae

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

Cite this

Isolation of microsatellite primers for Melampyrum sylvaticum (Orobanchaceae), an endangered plant in the United Kingdom. / Crichton, Rhiannon J.; Squirrell, Jane; Woodin, Sarah J.; Dalrymple, Sarah E.; Hollingsworth, Peter M.

In: American Journal of Botany, Vol. 99, No. 11, 19.11.2012, p. e457-e459.

Research output: Contribution to journalArticle

Crichton, Rhiannon J. ; Squirrell, Jane ; Woodin, Sarah J. ; Dalrymple, Sarah E. ; Hollingsworth, Peter M. / Isolation of microsatellite primers for Melampyrum sylvaticum (Orobanchaceae), an endangered plant in the United Kingdom. In: American Journal of Botany. 2012 ; Vol. 99, No. 11. pp. e457-e459.
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