Isolation, Polarization, and Expansion of CD4(+) Helper T Cell Lines and Clones Using Magnetic Beads

Lekh N Dahal, Robert N Barker, Frank J Ward

Research output: Contribution to journalArticle

Abstract

Autoreactive CD4(+) helper T cells specific for a range of nucleoprotein-derived autoantigens are an important feature of systemic lupus erythematosus, driving B cell differentiation and autoantibody production and contributing to the inflammatory lesions caused by immune complex deposition. Several peptide epitopes from nucleoprotein antigens have been identified and offer a means selectively to manipulate T cell responses by skewing toward a profile of cytokines that is less pro-inflammatory.Antigen-specific T cell lines and clones can be useful in the study of helper T cell subsets because their life span is prolonged and many individual cells can be generated, allowing particular phenotypes to be studied in detail. Magnetic beads offer a robust and convenient method for the isolation, polarization, and expansion of T cells, which can be adapted for a broad range of applications.
Original languageEnglish
Pages (from-to)237-247
Number of pages11
JournalMethods in Molecular Biology
Volume1134
DOIs
Publication statusPublished - 2014

Fingerprint

Helper-Inducer T-Lymphocytes
Nucleoproteins
Clone Cells
T-Lymphocytes
Cell Line
Antigens
Autoantigens
T-Lymphocyte Subsets
Antigen-Antibody Complex
Systemic Lupus Erythematosus
Autoantibodies
Epitopes
Cell Differentiation
B-Lymphocytes
Cytokines
Phenotype
Peptides

Keywords

  • helper T cell
  • T cell line
  • T cell clone
  • magnetic bead expansion
  • antigen specificity

Cite this

Isolation, Polarization, and Expansion of CD4(+) Helper T Cell Lines and Clones Using Magnetic Beads. / Dahal, Lekh N; Barker, Robert N; Ward, Frank J.

In: Methods in Molecular Biology, Vol. 1134, 2014, p. 237-247.

Research output: Contribution to journalArticle

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