Isolation, Polarization, and Expansion of CD4(+) Helper T Cell Lines and Clones Using Magnetic Beads

Lekh N Dahal, Robert N Barker, Frank J Ward

Research output: Contribution to journalArticle

Abstract

Autoreactive CD4(+) helper T cells specific for a range of nucleoprotein-derived autoantigens are an important feature of systemic lupus erythematosus, driving B cell differentiation and autoantibody production and contributing to the inflammatory lesions caused by immune complex deposition. Several peptide epitopes from nucleoprotein antigens have been identified and offer a means selectively to manipulate T cell responses by skewing toward a profile of cytokines that is less pro-inflammatory.Antigen-specific T cell lines and clones can be useful in the study of helper T cell subsets because their life span is prolonged and many individual cells can be generated, allowing particular phenotypes to be studied in detail. Magnetic beads offer a robust and convenient method for the isolation, polarization, and expansion of T cells, which can be adapted for a broad range of applications.
Original languageEnglish
Pages (from-to)237-247
Number of pages11
JournalMethods in Molecular Biology
Volume1134
DOIs
Publication statusPublished - 2014

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Keywords

  • helper T cell
  • T cell line
  • T cell clone
  • magnetic bead expansion
  • antigen specificity

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