Abstract
Autoreactive CD4(+) helper T cells specific for a range of nucleoprotein-derived autoantigens are an important feature of systemic lupus erythematosus, driving B cell differentiation and autoantibody production and contributing to the inflammatory lesions caused by immune complex deposition. Several peptide epitopes from nucleoprotein antigens have been identified and offer a means selectively to manipulate T cell responses by skewing toward a profile of cytokines that is less pro-inflammatory.Antigen-specific T cell lines and clones can be useful in the study of helper T cell subsets because their life span is prolonged and many individual cells can be generated, allowing particular phenotypes to be studied in detail. Magnetic beads offer a robust and convenient method for the isolation, polarization, and expansion of T cells, which can be adapted for a broad range of applications.
Original language | English |
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Pages (from-to) | 237-247 |
Number of pages | 11 |
Journal | Methods in Molecular Biology |
Volume | 1134 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- helper T cell
- T cell line
- T cell clone
- magnetic bead expansion
- antigen specificity