Steinernema kraussei (Steiner, 1923) (Rhabditida, Steinernematidae) from Iceland.

S. E. Spiridonov, E. Lyons, Michael John Wilson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A Survey of soils front Iceland for the presence of cold-active entoniopathogenic nematodes yielded a single isolate of Steinernema kraussei. This isolate wits compared morphologically with the topotype from Westphalia, Germany, and was found to be similar except that the strain from Iceland had shorter mucrons and it longer gubernaculum in second-generation males. A fragment of the ribosomal DNA (internal transcribed spacer 1 [ITS 1] + 5.8S + ITS2) wits amplified and sequenced along with 7 other isolates of S. kraussei, 2 isolates front Switzerland, 1 isolate from Scotland, and 4 isolates from Russia. Phylogenetic trees prepared using maximum parsimony methods placed the strain from Iceland ill a cluster with 2 Russian strains. The virulence of S. kraussei front Iceland against larvae of Tenebrio molitor was compared with thin of a commercially available strain of Steinernema feltiae at 12degreesC and 4degreesC. At 12degreesC the strain front Iceland Wits less virulent than S. feltiae, and at 4degreesC the strain from Iceland demonstrated no detectable virulence, whereas S. feltiae evinced limited virulence.

Original languageEnglish
Pages (from-to)215-220
Number of pages5
JournalComparative Parasitology
Volume71
Publication statusPublished - 2004

Keywords

  • Steinernema kraussei
  • morphology
  • ITS rDNA
  • Galleria mellonella baiting
  • Iceland
  • temperature activity
  • Tenebrio molitor
  • nematode
  • Rhabditida
  • steinernematidac

Cite this

Spiridonov, S. E., Lyons, E., & Wilson, M. J. (2004). Steinernema kraussei (Steiner, 1923) (Rhabditida, Steinernematidae) from Iceland. Comparative Parasitology, 71, 215-220.

Steinernema kraussei (Steiner, 1923) (Rhabditida, Steinernematidae) from Iceland. / Spiridonov, S. E.; Lyons, E.; Wilson, Michael John.

In: Comparative Parasitology, Vol. 71, 2004, p. 215-220.

Research output: Contribution to journalArticle

Spiridonov, SE, Lyons, E & Wilson, MJ 2004, 'Steinernema kraussei (Steiner, 1923) (Rhabditida, Steinernematidae) from Iceland.', Comparative Parasitology, vol. 71, pp. 215-220.
Spiridonov, S. E. ; Lyons, E. ; Wilson, Michael John. / Steinernema kraussei (Steiner, 1923) (Rhabditida, Steinernematidae) from Iceland. In: Comparative Parasitology. 2004 ; Vol. 71. pp. 215-220.
@article{69771a78746c4c3786f62a1fd38746a2,
title = "Steinernema kraussei (Steiner, 1923) (Rhabditida, Steinernematidae) from Iceland.",
abstract = "A Survey of soils front Iceland for the presence of cold-active entoniopathogenic nematodes yielded a single isolate of Steinernema kraussei. This isolate wits compared morphologically with the topotype from Westphalia, Germany, and was found to be similar except that the strain from Iceland had shorter mucrons and it longer gubernaculum in second-generation males. A fragment of the ribosomal DNA (internal transcribed spacer 1 [ITS 1] + 5.8S + ITS2) wits amplified and sequenced along with 7 other isolates of S. kraussei, 2 isolates front Switzerland, 1 isolate from Scotland, and 4 isolates from Russia. Phylogenetic trees prepared using maximum parsimony methods placed the strain from Iceland ill a cluster with 2 Russian strains. The virulence of S. kraussei front Iceland against larvae of Tenebrio molitor was compared with thin of a commercially available strain of Steinernema feltiae at 12degreesC and 4degreesC. At 12degreesC the strain front Iceland Wits less virulent than S. feltiae, and at 4degreesC the strain from Iceland demonstrated no detectable virulence, whereas S. feltiae evinced limited virulence.",
keywords = "Steinernema kraussei, morphology, ITS rDNA, Galleria mellonella baiting, Iceland, temperature activity, Tenebrio molitor, nematode, Rhabditida, steinernematidac",
author = "Spiridonov, {S. E.} and E. Lyons and Wilson, {Michael John}",
year = "2004",
language = "English",
volume = "71",
pages = "215--220",
journal = "Comparative Parasitology",
issn = "1525-2647",
publisher = "Helminthological Society of Washington",

}

TY - JOUR

T1 - Steinernema kraussei (Steiner, 1923) (Rhabditida, Steinernematidae) from Iceland.

AU - Spiridonov, S. E.

AU - Lyons, E.

AU - Wilson, Michael John

PY - 2004

Y1 - 2004

N2 - A Survey of soils front Iceland for the presence of cold-active entoniopathogenic nematodes yielded a single isolate of Steinernema kraussei. This isolate wits compared morphologically with the topotype from Westphalia, Germany, and was found to be similar except that the strain from Iceland had shorter mucrons and it longer gubernaculum in second-generation males. A fragment of the ribosomal DNA (internal transcribed spacer 1 [ITS 1] + 5.8S + ITS2) wits amplified and sequenced along with 7 other isolates of S. kraussei, 2 isolates front Switzerland, 1 isolate from Scotland, and 4 isolates from Russia. Phylogenetic trees prepared using maximum parsimony methods placed the strain from Iceland ill a cluster with 2 Russian strains. The virulence of S. kraussei front Iceland against larvae of Tenebrio molitor was compared with thin of a commercially available strain of Steinernema feltiae at 12degreesC and 4degreesC. At 12degreesC the strain front Iceland Wits less virulent than S. feltiae, and at 4degreesC the strain from Iceland demonstrated no detectable virulence, whereas S. feltiae evinced limited virulence.

AB - A Survey of soils front Iceland for the presence of cold-active entoniopathogenic nematodes yielded a single isolate of Steinernema kraussei. This isolate wits compared morphologically with the topotype from Westphalia, Germany, and was found to be similar except that the strain from Iceland had shorter mucrons and it longer gubernaculum in second-generation males. A fragment of the ribosomal DNA (internal transcribed spacer 1 [ITS 1] + 5.8S + ITS2) wits amplified and sequenced along with 7 other isolates of S. kraussei, 2 isolates front Switzerland, 1 isolate from Scotland, and 4 isolates from Russia. Phylogenetic trees prepared using maximum parsimony methods placed the strain from Iceland ill a cluster with 2 Russian strains. The virulence of S. kraussei front Iceland against larvae of Tenebrio molitor was compared with thin of a commercially available strain of Steinernema feltiae at 12degreesC and 4degreesC. At 12degreesC the strain front Iceland Wits less virulent than S. feltiae, and at 4degreesC the strain from Iceland demonstrated no detectable virulence, whereas S. feltiae evinced limited virulence.

KW - Steinernema kraussei

KW - morphology

KW - ITS rDNA

KW - Galleria mellonella baiting

KW - Iceland

KW - temperature activity

KW - Tenebrio molitor

KW - nematode

KW - Rhabditida

KW - steinernematidac

M3 - Article

VL - 71

SP - 215

EP - 220

JO - Comparative Parasitology

JF - Comparative Parasitology

SN - 1525-2647

ER -