Kidney Bean Lectin-Induced Escherichia-Coli Overgrowth in the Small-Inestine is Blocked by GNA, A Mannose-Specific Lectin

A PUSZTAI, George Grant, R J SPENCER, T J DUGUID, David Stanley Brown, S.w.b. Ewen, W J PEUMANS, E J M VANDAMME, S BARDOCZ

Research output: Contribution to journalArticle

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Abstract

The reversible and dose-dependent hyperplastic growth of the small intestine and accelerated epithelial cell turnover caused by feeding rats with diets containing kidney bean lectin (PHA) increased the proportion of immature cells on the villi whose membrane and/or cytoplasm contained mainly simple, polymannosylated glycans. These new alpha-linked mannosyl terminals, particularly of the damaged epithelium, facilitated the preferential adherence of opportunistic Escherichia coli with mannose-sensitive Type 1 fimbriae, and other coliforms, to the glycocalyx. Accordingly, the growth of the gut was accompanied by a reversible and PHA dose-dependent overgrowth with E.coli. As expected from their common carbohydrate specificity, the inclusion in the diet of the mannose-specific agglutinin from snowdrop (Galanthus nivalis) bulbs (GNA) significantly reduced the extent of E.coli overgrowth, but abolished neither the growth nor the damage caused by PHA to the small intestine. Thus, GNA and perhaps other mannose-specific lectins, especially when used in a preventive mode, can be used to specifically block the proliferation of Type 1 E.coli in the small intestine.

Original languageEnglish
Pages (from-to)360-368
Number of pages9
JournalJournal of Applied Bacteriology
Volume75
Issue number4
Publication statusPublished - Oct 1993

Keywords

  • rat small-intestine
  • phaseolus-Vulgaris
  • induced lesions
  • binding

Cite this

PUSZTAI, A., Grant, G., SPENCER, R. J., DUGUID, T. J., Brown, D. S., Ewen, S. W. B., ... BARDOCZ, S. (1993). Kidney Bean Lectin-Induced Escherichia-Coli Overgrowth in the Small-Inestine is Blocked by GNA, A Mannose-Specific Lectin. Journal of Applied Bacteriology, 75(4), 360-368.

Kidney Bean Lectin-Induced Escherichia-Coli Overgrowth in the Small-Inestine is Blocked by GNA, A Mannose-Specific Lectin. / PUSZTAI, A ; Grant, George; SPENCER, R J ; DUGUID, T J ; Brown, David Stanley; Ewen, S.w.b.; PEUMANS, W J ; VANDAMME, E J M ; BARDOCZ, S .

In: Journal of Applied Bacteriology, Vol. 75, No. 4, 10.1993, p. 360-368.

Research output: Contribution to journalArticle

PUSZTAI, A, Grant, G, SPENCER, RJ, DUGUID, TJ, Brown, DS, Ewen, SWB, PEUMANS, WJ, VANDAMME, EJM & BARDOCZ, S 1993, 'Kidney Bean Lectin-Induced Escherichia-Coli Overgrowth in the Small-Inestine is Blocked by GNA, A Mannose-Specific Lectin', Journal of Applied Bacteriology, vol. 75, no. 4, pp. 360-368.
PUSZTAI, A ; Grant, George ; SPENCER, R J ; DUGUID, T J ; Brown, David Stanley ; Ewen, S.w.b. ; PEUMANS, W J ; VANDAMME, E J M ; BARDOCZ, S . / Kidney Bean Lectin-Induced Escherichia-Coli Overgrowth in the Small-Inestine is Blocked by GNA, A Mannose-Specific Lectin. In: Journal of Applied Bacteriology. 1993 ; Vol. 75, No. 4. pp. 360-368.
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abstract = "The reversible and dose-dependent hyperplastic growth of the small intestine and accelerated epithelial cell turnover caused by feeding rats with diets containing kidney bean lectin (PHA) increased the proportion of immature cells on the villi whose membrane and/or cytoplasm contained mainly simple, polymannosylated glycans. These new alpha-linked mannosyl terminals, particularly of the damaged epithelium, facilitated the preferential adherence of opportunistic Escherichia coli with mannose-sensitive Type 1 fimbriae, and other coliforms, to the glycocalyx. Accordingly, the growth of the gut was accompanied by a reversible and PHA dose-dependent overgrowth with E.coli. As expected from their common carbohydrate specificity, the inclusion in the diet of the mannose-specific agglutinin from snowdrop (Galanthus nivalis) bulbs (GNA) significantly reduced the extent of E.coli overgrowth, but abolished neither the growth nor the damage caused by PHA to the small intestine. Thus, GNA and perhaps other mannose-specific lectins, especially when used in a preventive mode, can be used to specifically block the proliferation of Type 1 E.coli in the small intestine.",
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AB - The reversible and dose-dependent hyperplastic growth of the small intestine and accelerated epithelial cell turnover caused by feeding rats with diets containing kidney bean lectin (PHA) increased the proportion of immature cells on the villi whose membrane and/or cytoplasm contained mainly simple, polymannosylated glycans. These new alpha-linked mannosyl terminals, particularly of the damaged epithelium, facilitated the preferential adherence of opportunistic Escherichia coli with mannose-sensitive Type 1 fimbriae, and other coliforms, to the glycocalyx. Accordingly, the growth of the gut was accompanied by a reversible and PHA dose-dependent overgrowth with E.coli. As expected from their common carbohydrate specificity, the inclusion in the diet of the mannose-specific agglutinin from snowdrop (Galanthus nivalis) bulbs (GNA) significantly reduced the extent of E.coli overgrowth, but abolished neither the growth nor the damage caused by PHA to the small intestine. Thus, GNA and perhaps other mannose-specific lectins, especially when used in a preventive mode, can be used to specifically block the proliferation of Type 1 E.coli in the small intestine.

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