Kinetics of leukocyte and myeloid cell traffic in the murine corneal allograft response

Lucia Kuffova, Lynne Lumsden, V. Vesela, Julie Ann Taylor, M. Filipec, V. Holan, A. D. Dick, John Vincent Forrester

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background. Little information exists on the trafficking of myeloid and lymphoid cells between the transplanted cornea and the secondary lymphoid tissue. This study reports on changes in the cornea and the draining lymph node (DLN) from the time of graft emplacement.

Methods. Using a mouse corneal graft model (C57BL/ 10Sn to BALB/c), eyes and submandibular DLN were examined by immunohistochemistry and three-color flow cytometry for evidence of T cell activation and dendritic cell (DC) conditioning (up-regulation of costimulatory molecules) at various times (15 min to 24 days; n=4 for each time).

Results. In the DLN, early (2 hr) DC conditioning was sustained throughout allograft rejection whereas a remarkable drop in percentage of activated CD4(+) and CDS' T cells (P <0.001) was followed by a biphasic rise in activated CD4(+) and, to a lesser extent, CD8(+) T cells (24 hr, P <0.001 and 6 days, P <0.01). CD11b(+) and MOMA-2(+) macrophages, MHC Class II+ cells, CD86(+) DC, and neutrophils were the earliest cells infiltrating the cornea (at 24 hr), whereas T cells appeared after 2 days, with CD4(+) T cells being confined largely to the graft recipient border.

Conclusions. Immediate and rapid changes in T cell and DC populations in the DLN correlate with the type of cellular infiltration in the corneal graft. The data are consistent with a model in which CD4(+) T cell help for CD8(+) cytotoxic T cells could be provided by sequential two-way activation of T cells and DC in the DLN. The majority of cells infiltrating the graft were macrophages and neutrophils, with fewer DC and T cells.

Original languageEnglish
Pages (from-to)1292-1298
Number of pages6
JournalTransplantation
Volume72
Issue number7
DOIs
Publication statusPublished - 2001

Keywords

  • AUTOIMMUNE UVEORETINITIS
  • PENETRATING KERATOPLASTY
  • ADHESION MOLECULES
  • REJECTION
  • TRANSPLANTATION
  • RAT
  • MICE
  • EYE
  • INDUCTION
  • SELECTION

Cite this

Kuffova, L., Lumsden, L., Vesela, V., Taylor, J. A., Filipec, M., Holan, V., ... Forrester, J. V. (2001). Kinetics of leukocyte and myeloid cell traffic in the murine corneal allograft response. Transplantation, 72(7), 1292-1298. https://doi.org/10.1097/00007890-200110150-00019

Kinetics of leukocyte and myeloid cell traffic in the murine corneal allograft response. / Kuffova, Lucia; Lumsden, Lynne; Vesela, V.; Taylor, Julie Ann; Filipec, M.; Holan, V.; Dick, A. D.; Forrester, John Vincent.

In: Transplantation, Vol. 72, No. 7, 2001, p. 1292-1298.

Research output: Contribution to journalArticle

Kuffova, L, Lumsden, L, Vesela, V, Taylor, JA, Filipec, M, Holan, V, Dick, AD & Forrester, JV 2001, 'Kinetics of leukocyte and myeloid cell traffic in the murine corneal allograft response', Transplantation, vol. 72, no. 7, pp. 1292-1298. https://doi.org/10.1097/00007890-200110150-00019
Kuffova, Lucia ; Lumsden, Lynne ; Vesela, V. ; Taylor, Julie Ann ; Filipec, M. ; Holan, V. ; Dick, A. D. ; Forrester, John Vincent. / Kinetics of leukocyte and myeloid cell traffic in the murine corneal allograft response. In: Transplantation. 2001 ; Vol. 72, No. 7. pp. 1292-1298.
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abstract = "Background. Little information exists on the trafficking of myeloid and lymphoid cells between the transplanted cornea and the secondary lymphoid tissue. This study reports on changes in the cornea and the draining lymph node (DLN) from the time of graft emplacement.Methods. Using a mouse corneal graft model (C57BL/ 10Sn to BALB/c), eyes and submandibular DLN were examined by immunohistochemistry and three-color flow cytometry for evidence of T cell activation and dendritic cell (DC) conditioning (up-regulation of costimulatory molecules) at various times (15 min to 24 days; n=4 for each time).Results. In the DLN, early (2 hr) DC conditioning was sustained throughout allograft rejection whereas a remarkable drop in percentage of activated CD4(+) and CDS' T cells (P <0.001) was followed by a biphasic rise in activated CD4(+) and, to a lesser extent, CD8(+) T cells (24 hr, P <0.001 and 6 days, P <0.01). CD11b(+) and MOMA-2(+) macrophages, MHC Class II+ cells, CD86(+) DC, and neutrophils were the earliest cells infiltrating the cornea (at 24 hr), whereas T cells appeared after 2 days, with CD4(+) T cells being confined largely to the graft recipient border.Conclusions. Immediate and rapid changes in T cell and DC populations in the DLN correlate with the type of cellular infiltration in the corneal graft. The data are consistent with a model in which CD4(+) T cell help for CD8(+) cytotoxic T cells could be provided by sequential two-way activation of T cells and DC in the DLN. The majority of cells infiltrating the graft were macrophages and neutrophils, with fewer DC and T cells.",
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TY - JOUR

T1 - Kinetics of leukocyte and myeloid cell traffic in the murine corneal allograft response

AU - Kuffova, Lucia

AU - Lumsden, Lynne

AU - Vesela, V.

AU - Taylor, Julie Ann

AU - Filipec, M.

AU - Holan, V.

AU - Dick, A. D.

AU - Forrester, John Vincent

PY - 2001

Y1 - 2001

N2 - Background. Little information exists on the trafficking of myeloid and lymphoid cells between the transplanted cornea and the secondary lymphoid tissue. This study reports on changes in the cornea and the draining lymph node (DLN) from the time of graft emplacement.Methods. Using a mouse corneal graft model (C57BL/ 10Sn to BALB/c), eyes and submandibular DLN were examined by immunohistochemistry and three-color flow cytometry for evidence of T cell activation and dendritic cell (DC) conditioning (up-regulation of costimulatory molecules) at various times (15 min to 24 days; n=4 for each time).Results. In the DLN, early (2 hr) DC conditioning was sustained throughout allograft rejection whereas a remarkable drop in percentage of activated CD4(+) and CDS' T cells (P <0.001) was followed by a biphasic rise in activated CD4(+) and, to a lesser extent, CD8(+) T cells (24 hr, P <0.001 and 6 days, P <0.01). CD11b(+) and MOMA-2(+) macrophages, MHC Class II+ cells, CD86(+) DC, and neutrophils were the earliest cells infiltrating the cornea (at 24 hr), whereas T cells appeared after 2 days, with CD4(+) T cells being confined largely to the graft recipient border.Conclusions. Immediate and rapid changes in T cell and DC populations in the DLN correlate with the type of cellular infiltration in the corneal graft. The data are consistent with a model in which CD4(+) T cell help for CD8(+) cytotoxic T cells could be provided by sequential two-way activation of T cells and DC in the DLN. The majority of cells infiltrating the graft were macrophages and neutrophils, with fewer DC and T cells.

AB - Background. Little information exists on the trafficking of myeloid and lymphoid cells between the transplanted cornea and the secondary lymphoid tissue. This study reports on changes in the cornea and the draining lymph node (DLN) from the time of graft emplacement.Methods. Using a mouse corneal graft model (C57BL/ 10Sn to BALB/c), eyes and submandibular DLN were examined by immunohistochemistry and three-color flow cytometry for evidence of T cell activation and dendritic cell (DC) conditioning (up-regulation of costimulatory molecules) at various times (15 min to 24 days; n=4 for each time).Results. In the DLN, early (2 hr) DC conditioning was sustained throughout allograft rejection whereas a remarkable drop in percentage of activated CD4(+) and CDS' T cells (P <0.001) was followed by a biphasic rise in activated CD4(+) and, to a lesser extent, CD8(+) T cells (24 hr, P <0.001 and 6 days, P <0.01). CD11b(+) and MOMA-2(+) macrophages, MHC Class II+ cells, CD86(+) DC, and neutrophils were the earliest cells infiltrating the cornea (at 24 hr), whereas T cells appeared after 2 days, with CD4(+) T cells being confined largely to the graft recipient border.Conclusions. Immediate and rapid changes in T cell and DC populations in the DLN correlate with the type of cellular infiltration in the corneal graft. The data are consistent with a model in which CD4(+) T cell help for CD8(+) cytotoxic T cells could be provided by sequential two-way activation of T cells and DC in the DLN. The majority of cells infiltrating the graft were macrophages and neutrophils, with fewer DC and T cells.

KW - AUTOIMMUNE UVEORETINITIS

KW - PENETRATING KERATOPLASTY

KW - ADHESION MOLECULES

KW - REJECTION

KW - TRANSPLANTATION

KW - RAT

KW - MICE

KW - EYE

KW - INDUCTION

KW - SELECTION

U2 - 10.1097/00007890-200110150-00019

DO - 10.1097/00007890-200110150-00019

M3 - Article

VL - 72

SP - 1292

EP - 1298

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 7

ER -