Ku complex controls the replication time of DNA in telomere regions

Andrew Cosgrove, Conrad A Nieduszynski, Anne Dunlop Donaldson

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

We have investigated whether the Ku complex is involved in regulating DNA replication in the yeast Saccharomyces cerevisiae. We find that Ku proteins control the replication time of telomeric regions; replication origins located close to telomeres or within subtelomeric repeat sequences normally initiate late, but are activated much earlier in mutants lacking Ku function. In contrast, origins distant from telomeres initiate replication at the normal time. Ku is one of the first components identified as important for replication timing, and specification of the replication time of chromosome ends by Ku is consistent with its role in maintaining telomere localization.

Original languageEnglish
Pages (from-to)2485-2490
Number of pages5
JournalGenes & Development
Volume16
Issue number19
DOIs
Publication statusPublished - Oct 2002

Keywords

  • Ku
  • replication
  • origin
  • telomere
  • Saccharomyces cerevisiae
  • SACCHAROMYCES-CEREVISIAE
  • YEAST KU
  • S-PHASE
  • REPAIR PROTEIN
  • BUDDING YEAST
  • ORIGINS
  • ACTIVATION
  • POSITION
  • LOCALIZATION
  • ASSOCIATION

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