L-form bacteria of Pseudomonas syringae pv. phaseolicola induce chitinases and enhance resistance to Botrytis cinerea infection in Chinese cabbage

P. W. H. K. P. Daulagala, Eunice Jessie Allan

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Induction of chitinases in Chinese cabbage (Brassica campestris L. ssp. pekinensis var. Granaat) plants treated with L-form bacteria of Pseudomonas syringae pv. phaseolicola during seed germination, was investigated using a fluorometric assay with 4-methylumbelliferyl substrates. The constitutive chitinase activities of seeds and seedlings were low and contrasted with much higher chitinase activities in seedlings treated with L-form bacteria. Maximum chitinase activities of seedlings treated with L-form bacteria was found 31 days after treatment. Extracts of the seedlings treated with L-form bacteria showed 4-fold and 1.5-fold endochitinase activities with, 4-methylumbelliferyl beta-D-N-triacetylchitotrioside and 4-methylumbelliferyl beta-D-N-tetraacetylchitotetraoside, respectively. Plant/L-form association was detected using a slide agglutination assay, enzyme linked immuno-sorbent assay and by re-isolation of bacteria from seedlings. L-form bacteria were also observed in root hairs using light microscopy. Re-isolation showed that L-forms were systemically distributed throughout the plant, with the highest population in room This was supported by ELISA results. Chinese cabbage seedlings treated with L-form bacteria had enhanced resistance to infection by the grey mould pathogen Botrytis cinerea. (C) 2003 Elsevier Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)253-263
    Number of pages10
    JournalPhysiological & Molecular Plant Pathology
    Volume62
    DOIs
    Publication statusPublished - 2003

    Keywords

    • Brassica campestris L. ssp pekinensis
    • Pseudomonas syringae pv. phaseolicola
    • Botrytis cinerea Pers
    • L-form bacteria
    • plant association
    • symbioses
    • induced resistance
    • chitinase
    • PATHOGENESIS-RELATED PROTEINS
    • CHITINOLYTIC ACTIVITY
    • ANTIFUNGAL ACTIVITY
    • DEFENSE RESPONSES
    • FUNGAL GROWTH
    • BEAN-PLANTS
    • DISEASE
    • ASSOCIATIONS
    • PROTECTION
    • TISSUE

    Cite this

    L-form bacteria of Pseudomonas syringae pv. phaseolicola induce chitinases and enhance resistance to Botrytis cinerea infection in Chinese cabbage. / Daulagala, P. W. H. K. P.; Allan, Eunice Jessie.

    In: Physiological & Molecular Plant Pathology, Vol. 62, 2003, p. 253-263.

    Research output: Contribution to journalArticle

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    abstract = "Induction of chitinases in Chinese cabbage (Brassica campestris L. ssp. pekinensis var. Granaat) plants treated with L-form bacteria of Pseudomonas syringae pv. phaseolicola during seed germination, was investigated using a fluorometric assay with 4-methylumbelliferyl substrates. The constitutive chitinase activities of seeds and seedlings were low and contrasted with much higher chitinase activities in seedlings treated with L-form bacteria. Maximum chitinase activities of seedlings treated with L-form bacteria was found 31 days after treatment. Extracts of the seedlings treated with L-form bacteria showed 4-fold and 1.5-fold endochitinase activities with, 4-methylumbelliferyl beta-D-N-triacetylchitotrioside and 4-methylumbelliferyl beta-D-N-tetraacetylchitotetraoside, respectively. Plant/L-form association was detected using a slide agglutination assay, enzyme linked immuno-sorbent assay and by re-isolation of bacteria from seedlings. L-form bacteria were also observed in root hairs using light microscopy. Re-isolation showed that L-forms were systemically distributed throughout the plant, with the highest population in room This was supported by ELISA results. Chinese cabbage seedlings treated with L-form bacteria had enhanced resistance to infection by the grey mould pathogen Botrytis cinerea. (C) 2003 Elsevier Ltd. All rights reserved.",
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    T1 - L-form bacteria of Pseudomonas syringae pv. phaseolicola induce chitinases and enhance resistance to Botrytis cinerea infection in Chinese cabbage

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    AU - Allan, Eunice Jessie

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    N2 - Induction of chitinases in Chinese cabbage (Brassica campestris L. ssp. pekinensis var. Granaat) plants treated with L-form bacteria of Pseudomonas syringae pv. phaseolicola during seed germination, was investigated using a fluorometric assay with 4-methylumbelliferyl substrates. The constitutive chitinase activities of seeds and seedlings were low and contrasted with much higher chitinase activities in seedlings treated with L-form bacteria. Maximum chitinase activities of seedlings treated with L-form bacteria was found 31 days after treatment. Extracts of the seedlings treated with L-form bacteria showed 4-fold and 1.5-fold endochitinase activities with, 4-methylumbelliferyl beta-D-N-triacetylchitotrioside and 4-methylumbelliferyl beta-D-N-tetraacetylchitotetraoside, respectively. Plant/L-form association was detected using a slide agglutination assay, enzyme linked immuno-sorbent assay and by re-isolation of bacteria from seedlings. L-form bacteria were also observed in root hairs using light microscopy. Re-isolation showed that L-forms were systemically distributed throughout the plant, with the highest population in room This was supported by ELISA results. Chinese cabbage seedlings treated with L-form bacteria had enhanced resistance to infection by the grey mould pathogen Botrytis cinerea. (C) 2003 Elsevier Ltd. All rights reserved.

    AB - Induction of chitinases in Chinese cabbage (Brassica campestris L. ssp. pekinensis var. Granaat) plants treated with L-form bacteria of Pseudomonas syringae pv. phaseolicola during seed germination, was investigated using a fluorometric assay with 4-methylumbelliferyl substrates. The constitutive chitinase activities of seeds and seedlings were low and contrasted with much higher chitinase activities in seedlings treated with L-form bacteria. Maximum chitinase activities of seedlings treated with L-form bacteria was found 31 days after treatment. Extracts of the seedlings treated with L-form bacteria showed 4-fold and 1.5-fold endochitinase activities with, 4-methylumbelliferyl beta-D-N-triacetylchitotrioside and 4-methylumbelliferyl beta-D-N-tetraacetylchitotetraoside, respectively. Plant/L-form association was detected using a slide agglutination assay, enzyme linked immuno-sorbent assay and by re-isolation of bacteria from seedlings. L-form bacteria were also observed in root hairs using light microscopy. Re-isolation showed that L-forms were systemically distributed throughout the plant, with the highest population in room This was supported by ELISA results. Chinese cabbage seedlings treated with L-form bacteria had enhanced resistance to infection by the grey mould pathogen Botrytis cinerea. (C) 2003 Elsevier Ltd. All rights reserved.

    KW - Brassica campestris L. ssp pekinensis

    KW - Pseudomonas syringae pv. phaseolicola

    KW - Botrytis cinerea Pers

    KW - L-form bacteria

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    KW - symbioses

    KW - induced resistance

    KW - chitinase

    KW - PATHOGENESIS-RELATED PROTEINS

    KW - CHITINOLYTIC ACTIVITY

    KW - ANTIFUNGAL ACTIVITY

    KW - DEFENSE RESPONSES

    KW - FUNGAL GROWTH

    KW - BEAN-PLANTS

    KW - DISEASE

    KW - ASSOCIATIONS

    KW - PROTECTION

    KW - TISSUE

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    DO - 10.1016/S0885-5765(03)00089-4

    M3 - Article

    VL - 62

    SP - 253

    EP - 263

    JO - Physiological & Molecular Plant Pathology

    JF - Physiological & Molecular Plant Pathology

    SN - 0885-5765

    ER -