Labelling of Candida auris Cell Walls to Examine Levels of PAMP Exposure by Flow Cytometry and Fluorescence Microscopy

Chloe Pelletier, Alexander Lorenz* (Corresponding Author)

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Pathogen-associated molecular patterns (PAMPs) of the fungal cell wall are primary targets for the innate immune system of animals. Therefore, characterizing PAMP exposure of fungal pathogens helps to elucidate how they interact with their hosts at a molecular level. Fluorescent labelling can be used to monitor exposure of multiple fungal cell wall PAMPs in a single experiment. Here, we describe a protocol to simultaneously label chitin, mannan, and β-1,3-glucan in Candida auris to study these PAMPs by fluorescence microscopy and allow high-throughput examination of their exposure by flow cytometry.

Original languageEnglish
Title of host publicationCandida auris
Subtitle of host publicationMethods and Protocols
EditorsAlexander Lorenz
Place of PublicationTotawa New Jersey
PublisherSpringer
Chapter14
Pages179-187
Number of pages9
ISBN (Electronic)978-1-0716-2417-3
ISBN (Print)978-1-0716-2416-6
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2517
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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