Abstract
Pathogen-associated molecular patterns (PAMPs) of the fungal cell wall are primary targets for the innate immune system of animals. Therefore, characterizing PAMP exposure of fungal pathogens helps to elucidate how they interact with their hosts at a molecular level. Fluorescent labelling can be used to monitor exposure of multiple fungal cell wall PAMPs in a single experiment. Here, we describe a protocol to simultaneously label chitin, mannan, and β-1,3-glucan in Candida auris to study these PAMPs by fluorescence microscopy and allow high-throughput examination of their exposure by flow cytometry.
Original language | English |
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Title of host publication | Candida auris |
Subtitle of host publication | Methods and Protocols |
Editors | Alexander Lorenz |
Place of Publication | Totawa New Jersey |
Publisher | Springer |
Chapter | 14 |
Pages | 179-187 |
Number of pages | 9 |
ISBN (Electronic) | 978-1-0716-2417-3 |
ISBN (Print) | 978-1-0716-2416-6 |
DOIs | |
Publication status | Published - 2022 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 2517 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
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Dive into the research topics of 'Labelling of Candida auris Cell Walls to Examine Levels of PAMP Exposure by Flow Cytometry and Fluorescence Microscopy'. Together they form a unique fingerprint.Equipment
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Iain Fraser Cytometry Centre
Andrea Holme (Manager), Linda Duncan (Senior Application Scientist), Ailsa Laird (Technician) & Kate Burgoyne (Technician)
Institute of Medical SciencesResearch Facilities: Facility
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Microscopy and Histology
Debbie Wilkinson (Manager) & Gillian Milne (Manager)
Medical SciencesResearch Facilities: Facility