Lack of flagella disadvantages Salmonella enterica serovar Enteritidis during the early stages of infection in the rat

J M C Robertson, N H McKenzie, M Duncan, E Allen-Vercoe, M J Woodward, H J Flint, G Grant

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The roles of flagella and five fimbriae (SEF14, SEF17, SEF21, pef, lpf) in the early stages (up to 3 days) of Salmonella enterica serovar Enteritidis (S. Enteritidis) infection have been investigated in the rat. Wild-type strains LA5 and S1400 (fim+/fla+) and insertionally inactivated mutants unable to express the five fimbriae (fim-/fla+), flagella (fim+/fla-) or fimbriae and flagella (fim-/fla-) were used. All wild-type and mutant strains were able to colonize the gut and spread to the mesenteric lymph nodes, liver and spleen. There appeared to be little or no difference between the fim-/fla+ and wild-type (fim+/fla+) strains. In contrast, the numbers of aflagellate (fim+/fla- or fim-/fla-) salmonella in the liver and spleen were transiently reduced. In addition, fim+/fla- or fim-/fla-strains were less able to persist in the upper gastrointestinal tract and the inflammatory responses they elicited in the gut were less severe. Thus, expression of SEF14, SEF17, SEF21, pef and lpf did not appear to be a prerequisite for induction of S. Enteritidis infection in the rat. Deletion of flagella did, however, disadvantage the bacterium. This may be due to the inability to produce or release the potent immunomodulating protein flagellin.

Original languageEnglish
Pages (from-to)91-99
Number of pages9
JournalJournal of Medical Microbiology
Volume52
Issue number1
DOIs
Publication statusPublished - Jan 2003

Keywords

  • serotype enteritidis
  • epithelial-cells
  • in-vivo
  • intestinal-mucosa
  • typhimurium
  • model
  • fimbriae
  • virulence
  • colonization
  • mice

Cite this

Lack of flagella disadvantages Salmonella enterica serovar Enteritidis during the early stages of infection in the rat. / Robertson, J M C ; McKenzie, N H ; Duncan, M ; Allen-Vercoe, E ; Woodward, M J ; Flint, H J ; Grant, G .

In: Journal of Medical Microbiology, Vol. 52, No. 1, 01.2003, p. 91-99.

Research output: Contribution to journalArticle

Robertson, J M C ; McKenzie, N H ; Duncan, M ; Allen-Vercoe, E ; Woodward, M J ; Flint, H J ; Grant, G . / Lack of flagella disadvantages Salmonella enterica serovar Enteritidis during the early stages of infection in the rat. In: Journal of Medical Microbiology. 2003 ; Vol. 52, No. 1. pp. 91-99.
@article{cb27c955cd3f438abc96404e6cc2fa8d,
title = "Lack of flagella disadvantages Salmonella enterica serovar Enteritidis during the early stages of infection in the rat",
abstract = "The roles of flagella and five fimbriae (SEF14, SEF17, SEF21, pef, lpf) in the early stages (up to 3 days) of Salmonella enterica serovar Enteritidis (S. Enteritidis) infection have been investigated in the rat. Wild-type strains LA5 and S1400 (fim+/fla+) and insertionally inactivated mutants unable to express the five fimbriae (fim-/fla+), flagella (fim+/fla-) or fimbriae and flagella (fim-/fla-) were used. All wild-type and mutant strains were able to colonize the gut and spread to the mesenteric lymph nodes, liver and spleen. There appeared to be little or no difference between the fim-/fla+ and wild-type (fim+/fla+) strains. In contrast, the numbers of aflagellate (fim+/fla- or fim-/fla-) salmonella in the liver and spleen were transiently reduced. In addition, fim+/fla- or fim-/fla-strains were less able to persist in the upper gastrointestinal tract and the inflammatory responses they elicited in the gut were less severe. Thus, expression of SEF14, SEF17, SEF21, pef and lpf did not appear to be a prerequisite for induction of S. Enteritidis infection in the rat. Deletion of flagella did, however, disadvantage the bacterium. This may be due to the inability to produce or release the potent immunomodulating protein flagellin.",
keywords = "serotype enteritidis, epithelial-cells, in-vivo, intestinal-mucosa, typhimurium, model, fimbriae, virulence, colonization, mice",
author = "Robertson, {J M C} and McKenzie, {N H} and M Duncan and E Allen-Vercoe and Woodward, {M J} and Flint, {H J} and G Grant",
year = "2003",
month = "1",
doi = "10.1099/jmm.0.04901-0",
language = "English",
volume = "52",
pages = "91--99",
journal = "Journal of Medical Microbiology",
issn = "0022-2615",
publisher = "Society for General Microbiology",
number = "1",

}

TY - JOUR

T1 - Lack of flagella disadvantages Salmonella enterica serovar Enteritidis during the early stages of infection in the rat

AU - Robertson, J M C

AU - McKenzie, N H

AU - Duncan, M

AU - Allen-Vercoe, E

AU - Woodward, M J

AU - Flint, H J

AU - Grant, G

PY - 2003/1

Y1 - 2003/1

N2 - The roles of flagella and five fimbriae (SEF14, SEF17, SEF21, pef, lpf) in the early stages (up to 3 days) of Salmonella enterica serovar Enteritidis (S. Enteritidis) infection have been investigated in the rat. Wild-type strains LA5 and S1400 (fim+/fla+) and insertionally inactivated mutants unable to express the five fimbriae (fim-/fla+), flagella (fim+/fla-) or fimbriae and flagella (fim-/fla-) were used. All wild-type and mutant strains were able to colonize the gut and spread to the mesenteric lymph nodes, liver and spleen. There appeared to be little or no difference between the fim-/fla+ and wild-type (fim+/fla+) strains. In contrast, the numbers of aflagellate (fim+/fla- or fim-/fla-) salmonella in the liver and spleen were transiently reduced. In addition, fim+/fla- or fim-/fla-strains were less able to persist in the upper gastrointestinal tract and the inflammatory responses they elicited in the gut were less severe. Thus, expression of SEF14, SEF17, SEF21, pef and lpf did not appear to be a prerequisite for induction of S. Enteritidis infection in the rat. Deletion of flagella did, however, disadvantage the bacterium. This may be due to the inability to produce or release the potent immunomodulating protein flagellin.

AB - The roles of flagella and five fimbriae (SEF14, SEF17, SEF21, pef, lpf) in the early stages (up to 3 days) of Salmonella enterica serovar Enteritidis (S. Enteritidis) infection have been investigated in the rat. Wild-type strains LA5 and S1400 (fim+/fla+) and insertionally inactivated mutants unable to express the five fimbriae (fim-/fla+), flagella (fim+/fla-) or fimbriae and flagella (fim-/fla-) were used. All wild-type and mutant strains were able to colonize the gut and spread to the mesenteric lymph nodes, liver and spleen. There appeared to be little or no difference between the fim-/fla+ and wild-type (fim+/fla+) strains. In contrast, the numbers of aflagellate (fim+/fla- or fim-/fla-) salmonella in the liver and spleen were transiently reduced. In addition, fim+/fla- or fim-/fla-strains were less able to persist in the upper gastrointestinal tract and the inflammatory responses they elicited in the gut were less severe. Thus, expression of SEF14, SEF17, SEF21, pef and lpf did not appear to be a prerequisite for induction of S. Enteritidis infection in the rat. Deletion of flagella did, however, disadvantage the bacterium. This may be due to the inability to produce or release the potent immunomodulating protein flagellin.

KW - serotype enteritidis

KW - epithelial-cells

KW - in-vivo

KW - intestinal-mucosa

KW - typhimurium

KW - model

KW - fimbriae

KW - virulence

KW - colonization

KW - mice

U2 - 10.1099/jmm.0.04901-0

DO - 10.1099/jmm.0.04901-0

M3 - Article

VL - 52

SP - 91

EP - 99

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

IS - 1

ER -