Lactobacillus reuteri 6475 increases bone density in intact females only under an inflammatory setting

Fraser L. Collins, Regina Irwin, Hayley Bierhalter, Jonathan Schepper, Robert A. Britton, Narayanan Parameswaran, Laura R. McCabe (Corresponding Author)

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Abstract

BACKGROUND & AIMS We previously demonstrated that short-term oral administration of the probiotic Lactobacillus reuteri 6475 enhanced bone density in male but not female mice. We also established that L. reuteri 6475 enhanced bone health and prevented bone loss in estrogen-deficient female mice. In this study, we tested whether a mild inflammatory state and/or a long-term treatment with the probiotic was required to promote a positive bone effect in estrogen-sufficient female mice. METHODS A mild inflammatory state was induced in female mice by dorsal surgical incision (DSI). Following DSI animals were orally supplemented with L. reuteri or vehicle control for a period of 8 weeks. Gene expression was measured in the intestine and bone marrow by qPCR. Distal femoral bone density and architecture was analyzed by micro-CT. RESULTS We report that 8 weeks after DSI there is a significant increase in the weight of spleen, thymus and visceral (retroperitoneal) fat pads. Expression of intestinal cytokines and tight junction proteins are also altered 8 weeks post-DSI. Interestingly, L. reuteri treatment was found to display both intestinal region- and inflammation-dependent effects. Unexpectedly we identified that 1) L. reuteri treatment increased bone density in females but only in those that underwent DSI and 2) DSI benefited cortical bone parameters. In the bone marrow, dorsal surgery induced CD4+ T cell numbers, a response that was unaffected by L. reuteri treatment, whereas expression of RANKL, OPG and IL-10 were significantly affected by L. reuteri treatment. CONCLUSION Our data reveals a previously unappreciated effect of a mild surgical procedure causing a long-lasting effect on inflammatory gene expression in the gut and the bone. Additionally, we demonstrate that in intact female mice, the beneficial effect of L. reuteri on bone requires an elevated inflammatory status.
Original languageEnglish
Article numbere0153180
JournalPloS ONE
Volume11
Issue number4
DOIs
Publication statusPublished - 8 Apr 2016

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Lactobacillus reuteri
bone density
Bone Density
Bone
bones
Bone and Bones
mice
Intra-Abdominal Fat
Probiotics
bone marrow
probiotics
estrogens
Estrogens
surgery
Bone Marrow
Gene expression
Tight Junction Proteins
Gene Expression
micro-computed tomography
gene expression

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Collins, F. L., Irwin, R., Bierhalter, H., Schepper, J., Britton, R. A., Parameswaran, N., & McCabe, L. R. (2016). Lactobacillus reuteri 6475 increases bone density in intact females only under an inflammatory setting. PloS ONE, 11(4), [e0153180]. https://doi.org/10.1371/journal.pone.0153180

Lactobacillus reuteri 6475 increases bone density in intact females only under an inflammatory setting. / Collins, Fraser L.; Irwin, Regina; Bierhalter, Hayley; Schepper, Jonathan; Britton, Robert A.; Parameswaran, Narayanan; McCabe, Laura R. (Corresponding Author).

In: PloS ONE, Vol. 11, No. 4, e0153180, 08.04.2016.

Research output: Contribution to journalArticle

Collins, FL, Irwin, R, Bierhalter, H, Schepper, J, Britton, RA, Parameswaran, N & McCabe, LR 2016, 'Lactobacillus reuteri 6475 increases bone density in intact females only under an inflammatory setting', PloS ONE, vol. 11, no. 4, e0153180. https://doi.org/10.1371/journal.pone.0153180
Collins, Fraser L. ; Irwin, Regina ; Bierhalter, Hayley ; Schepper, Jonathan ; Britton, Robert A. ; Parameswaran, Narayanan ; McCabe, Laura R. / Lactobacillus reuteri 6475 increases bone density in intact females only under an inflammatory setting. In: PloS ONE. 2016 ; Vol. 11, No. 4.
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