Lactobacillus rhamnosus strain GG reduces aflatoxin B-1 transport, metabolism, and toxicity in caco-2 cells

Silvia Gratz, Q. K. Wu, H. El-Nezami, R. O. Juvonen, H. Mykkanen, P. C. Turner

Research output: Contribution to journalArticle

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Abstract

The probiotic Lactobacillus rhamnosus GG is able to bind the potent hepatocarcinogen aflatoxin B, (AFB1) and thus potentially restrict its rapid absorption from the intestine. In this study we investigated the potential of GG to reduce AFB, availability in vitro in Caco-2 cells adapted to express cytochrome P-450 (CYP) 3A4, such that both transport and toxicity could be assessed. Caco-2 cells were grown as confluent monolayers on transmembrane filters for 21 days prior to all studies. AFB, levels in culture medium were measured by high-performance liquid chromatography. In CYP 3A4-induced monolayers, AFB(1) transport from the apical to the basolateral chamber was reduced from 11.1% +/- 1.9% to 6.4% +/- 2.5% (P = 0.019) and to 3.3% +/- 1.8% (P = 0.002) within the first hour in monolayers coincubated with GG (1 X 10(10) and 5 X 10(10) CFU/ml, respectively). GG (1 X 10(10) and 5 x 10(10) CFU/ml) bound 40.1% +/- 8.3% and 61.0% +/- 6.0% of added AFB(1) after 1 h, respectively. AFB, caused significant reductions of 30.1% (P = 0.01), 49.4% (P = 0.004), and 64.4% (P < 0.001) in transepithelial resistance after 24, 48, and 72 h, respectively. Coincubation with 1 X 10(10) CFU/ml GG after 24 h protected against AFB(1)-induced reductions in transepithelial resistance at both 24 h (P = 0.002) and 48 h (P = 0.04). DNA fragmentation was apparent in cells treated only with AFB, cells but not in cells coincubated with either 1 X 10(10) or 5 X 10(10) CFU/ml GG. GG reduced AFB1 uptake and protected against both membrane and DNA damage in the Caco-2 model. These data are suggestive of a beneficial role of GG against dietary exposure to aflatoxin.

Original languageEnglish
Pages (from-to)3958-3964
Number of pages7
JournalApplied and Environmental Microbiology
Volume73
Issue number12
DOIs
Publication statusPublished - Jun 2007

Keywords

  • lactic-acid bacteria
  • dairy strains
  • west-Africa
  • in-vitro
  • intestinal barrier
  • dietary aflatoxin
  • Gambian children
  • remove aflatoxin
  • drug transport
  • exposure

Cite this

Lactobacillus rhamnosus strain GG reduces aflatoxin B-1 transport, metabolism, and toxicity in caco-2 cells. / Gratz, Silvia; Wu, Q. K.; El-Nezami, H.; Juvonen, R. O.; Mykkanen, H.; Turner, P. C.

In: Applied and Environmental Microbiology, Vol. 73, No. 12, 06.2007, p. 3958-3964.

Research output: Contribution to journalArticle

Gratz, Silvia ; Wu, Q. K. ; El-Nezami, H. ; Juvonen, R. O. ; Mykkanen, H. ; Turner, P. C. / Lactobacillus rhamnosus strain GG reduces aflatoxin B-1 transport, metabolism, and toxicity in caco-2 cells. In: Applied and Environmental Microbiology. 2007 ; Vol. 73, No. 12. pp. 3958-3964.
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T1 - Lactobacillus rhamnosus strain GG reduces aflatoxin B-1 transport, metabolism, and toxicity in caco-2 cells

AU - Gratz, Silvia

AU - Wu, Q. K.

AU - El-Nezami, H.

AU - Juvonen, R. O.

AU - Mykkanen, H.

AU - Turner, P. C.

PY - 2007/6

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N2 - The probiotic Lactobacillus rhamnosus GG is able to bind the potent hepatocarcinogen aflatoxin B, (AFB1) and thus potentially restrict its rapid absorption from the intestine. In this study we investigated the potential of GG to reduce AFB, availability in vitro in Caco-2 cells adapted to express cytochrome P-450 (CYP) 3A4, such that both transport and toxicity could be assessed. Caco-2 cells were grown as confluent monolayers on transmembrane filters for 21 days prior to all studies. AFB, levels in culture medium were measured by high-performance liquid chromatography. In CYP 3A4-induced monolayers, AFB(1) transport from the apical to the basolateral chamber was reduced from 11.1% +/- 1.9% to 6.4% +/- 2.5% (P = 0.019) and to 3.3% +/- 1.8% (P = 0.002) within the first hour in monolayers coincubated with GG (1 X 10(10) and 5 X 10(10) CFU/ml, respectively). GG (1 X 10(10) and 5 x 10(10) CFU/ml) bound 40.1% +/- 8.3% and 61.0% +/- 6.0% of added AFB(1) after 1 h, respectively. AFB, caused significant reductions of 30.1% (P = 0.01), 49.4% (P = 0.004), and 64.4% (P < 0.001) in transepithelial resistance after 24, 48, and 72 h, respectively. Coincubation with 1 X 10(10) CFU/ml GG after 24 h protected against AFB(1)-induced reductions in transepithelial resistance at both 24 h (P = 0.002) and 48 h (P = 0.04). DNA fragmentation was apparent in cells treated only with AFB, cells but not in cells coincubated with either 1 X 10(10) or 5 X 10(10) CFU/ml GG. GG reduced AFB1 uptake and protected against both membrane and DNA damage in the Caco-2 model. These data are suggestive of a beneficial role of GG against dietary exposure to aflatoxin.

AB - The probiotic Lactobacillus rhamnosus GG is able to bind the potent hepatocarcinogen aflatoxin B, (AFB1) and thus potentially restrict its rapid absorption from the intestine. In this study we investigated the potential of GG to reduce AFB, availability in vitro in Caco-2 cells adapted to express cytochrome P-450 (CYP) 3A4, such that both transport and toxicity could be assessed. Caco-2 cells were grown as confluent monolayers on transmembrane filters for 21 days prior to all studies. AFB, levels in culture medium were measured by high-performance liquid chromatography. In CYP 3A4-induced monolayers, AFB(1) transport from the apical to the basolateral chamber was reduced from 11.1% +/- 1.9% to 6.4% +/- 2.5% (P = 0.019) and to 3.3% +/- 1.8% (P = 0.002) within the first hour in monolayers coincubated with GG (1 X 10(10) and 5 X 10(10) CFU/ml, respectively). GG (1 X 10(10) and 5 x 10(10) CFU/ml) bound 40.1% +/- 8.3% and 61.0% +/- 6.0% of added AFB(1) after 1 h, respectively. AFB, caused significant reductions of 30.1% (P = 0.01), 49.4% (P = 0.004), and 64.4% (P < 0.001) in transepithelial resistance after 24, 48, and 72 h, respectively. Coincubation with 1 X 10(10) CFU/ml GG after 24 h protected against AFB(1)-induced reductions in transepithelial resistance at both 24 h (P = 0.002) and 48 h (P = 0.04). DNA fragmentation was apparent in cells treated only with AFB, cells but not in cells coincubated with either 1 X 10(10) or 5 X 10(10) CFU/ml GG. GG reduced AFB1 uptake and protected against both membrane and DNA damage in the Caco-2 model. These data are suggestive of a beneficial role of GG against dietary exposure to aflatoxin.

KW - lactic-acid bacteria

KW - dairy strains

KW - west-Africa

KW - in-vitro

KW - intestinal barrier

KW - dietary aflatoxin

KW - Gambian children

KW - remove aflatoxin

KW - drug transport

KW - exposure

U2 - 10.1128/AEM.02944-06

DO - 10.1128/AEM.02944-06

M3 - Article

VL - 73

SP - 3958

EP - 3964

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 12

ER -