Lactobacillus rhamnosus strain GG restores alkaline phosphatase activity in differentiating Caco-2 cells dosed with the potent mycotoxin deoxynivalenol

P. C. Turner, Q. K. Wu, S. Piekkola, Silvia Gratz, H. Mykkanen, H. El-Nezami

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Deoxynivalenol (DON) contamination of cereal crops occurs frequently, and may cause acute exposure at high levels or chronic more moderate exposure. DON has proven toxicity including restriction of enterocyte differentiation, which may play a part in DON induced gastroenteritis. The probiotic bacteria Lactobacillus rhamnosus strain GG (GG) can bind DON, and therefore potentially restrict bioavailability of this toxin. Binding efficacy is not significantly altered by heat treatment, and therefore this in vitro study evaluated whether heat inactivated GG could restore the differentiation process in Caco-2 cells, using alkaline phosphatase (ALP) activity as a marker of differentiation. DON (200 ng/mL) caused a significant (p < 0.001) 36% reduction in ALP activity (1598 +/- 137 U/mg protein) compared to untreated cells (2502 +/- 80 U/mg). A dose dependant restoration of ALP activity was observed where DON treated cells were co-incubated with heat inactivated GG (1719 +/- 84; 2007 +/- 142; 2272 +/- 160 U/mg for GG at 1 x 10(4) (P > 0.9), 1 x 10(7) (p < 0.001), and 1 x 10(10) CFU/mL (p < 0.001), respectively). Co-incubation of the non-binding strain, LC-705 (1 x 10(10) CFU/mL), with DON did not significantly restore the ALP (1841 +/- 97 U/mg, p < 0.077) compared to DON only treated cells. When viable GG were co-incubated with DON a similar restoration of ALP activity was observed as seen for heat inactivated GG. These combined data suggest that the major effect of GG on restoring ALP activity, and therefore Caco-2 cell differentiation, was due to specific binding of DON, with possibly a more minor role of non-specific bacterial interference. (C) 2008 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)2118-2123
Number of pages6
JournalFood and Chemical Toxicology
Volume46
Issue number6
Early online date9 Feb 2008
DOIs
Publication statusPublished - Jun 2008

Keywords

  • Lactobacillus
  • probiotic
  • deoxynivalenol
  • Caco-2
  • intestine
  • sodium-butyrate
  • in-vitro
  • lines
  • culture
  • immunomodulation
  • deepoxidation
  • polarization
  • metabolism
  • toxicology
  • transport

Cite this

Lactobacillus rhamnosus strain GG restores alkaline phosphatase activity in differentiating Caco-2 cells dosed with the potent mycotoxin deoxynivalenol. / Turner, P. C.; Wu, Q. K.; Piekkola, S.; Gratz, Silvia; Mykkanen, H.; El-Nezami, H.

In: Food and Chemical Toxicology, Vol. 46, No. 6, 06.2008, p. 2118-2123.

Research output: Contribution to journalArticle

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abstract = "Deoxynivalenol (DON) contamination of cereal crops occurs frequently, and may cause acute exposure at high levels or chronic more moderate exposure. DON has proven toxicity including restriction of enterocyte differentiation, which may play a part in DON induced gastroenteritis. The probiotic bacteria Lactobacillus rhamnosus strain GG (GG) can bind DON, and therefore potentially restrict bioavailability of this toxin. Binding efficacy is not significantly altered by heat treatment, and therefore this in vitro study evaluated whether heat inactivated GG could restore the differentiation process in Caco-2 cells, using alkaline phosphatase (ALP) activity as a marker of differentiation. DON (200 ng/mL) caused a significant (p < 0.001) 36{\%} reduction in ALP activity (1598 +/- 137 U/mg protein) compared to untreated cells (2502 +/- 80 U/mg). A dose dependant restoration of ALP activity was observed where DON treated cells were co-incubated with heat inactivated GG (1719 +/- 84; 2007 +/- 142; 2272 +/- 160 U/mg for GG at 1 x 10(4) (P > 0.9), 1 x 10(7) (p < 0.001), and 1 x 10(10) CFU/mL (p < 0.001), respectively). Co-incubation of the non-binding strain, LC-705 (1 x 10(10) CFU/mL), with DON did not significantly restore the ALP (1841 +/- 97 U/mg, p < 0.077) compared to DON only treated cells. When viable GG were co-incubated with DON a similar restoration of ALP activity was observed as seen for heat inactivated GG. These combined data suggest that the major effect of GG on restoring ALP activity, and therefore Caco-2 cell differentiation, was due to specific binding of DON, with possibly a more minor role of non-specific bacterial interference. (C) 2008 Elsevier Ltd. All rights reserved.",
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AU - Wu, Q. K.

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AB - Deoxynivalenol (DON) contamination of cereal crops occurs frequently, and may cause acute exposure at high levels or chronic more moderate exposure. DON has proven toxicity including restriction of enterocyte differentiation, which may play a part in DON induced gastroenteritis. The probiotic bacteria Lactobacillus rhamnosus strain GG (GG) can bind DON, and therefore potentially restrict bioavailability of this toxin. Binding efficacy is not significantly altered by heat treatment, and therefore this in vitro study evaluated whether heat inactivated GG could restore the differentiation process in Caco-2 cells, using alkaline phosphatase (ALP) activity as a marker of differentiation. DON (200 ng/mL) caused a significant (p < 0.001) 36% reduction in ALP activity (1598 +/- 137 U/mg protein) compared to untreated cells (2502 +/- 80 U/mg). A dose dependant restoration of ALP activity was observed where DON treated cells were co-incubated with heat inactivated GG (1719 +/- 84; 2007 +/- 142; 2272 +/- 160 U/mg for GG at 1 x 10(4) (P > 0.9), 1 x 10(7) (p < 0.001), and 1 x 10(10) CFU/mL (p < 0.001), respectively). Co-incubation of the non-binding strain, LC-705 (1 x 10(10) CFU/mL), with DON did not significantly restore the ALP (1841 +/- 97 U/mg, p < 0.077) compared to DON only treated cells. When viable GG were co-incubated with DON a similar restoration of ALP activity was observed as seen for heat inactivated GG. These combined data suggest that the major effect of GG on restoring ALP activity, and therefore Caco-2 cell differentiation, was due to specific binding of DON, with possibly a more minor role of non-specific bacterial interference. (C) 2008 Elsevier Ltd. All rights reserved.

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KW - in-vitro

KW - lines

KW - culture

KW - immunomodulation

KW - deepoxidation

KW - polarization

KW - metabolism

KW - toxicology

KW - transport

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DO - 10.1016/j.fct.2008.02.004

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VL - 46

SP - 2118

EP - 2123

JO - Food and Chemical Toxicology

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ER -