Large plasmids in ruminal strains of Selenomonas ruminantium

K Fliegerova, O Benada, H J Flint

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Abstract

The plasmid content of six different isolates of Selenomonas ruminantium from the rumen of sheep, cows or goats was examined by electron microscopy. In addition to small plasmids (< 12 kb) studied previously, all six strains contained at least one plasmid larger than 20 kb. Plasmid sizes of 1.4, 2.1, 2.4, 5.0, 6.2, 20.4, 20.8, 22.7, 33.3, 29.3, 30.7, 34.4 and 42.6 kb were estimated from contour length measurements. DNA-DNA hybridization experiments revealed homology among the large plasmids from five strains, while the 20.8 kb plasmid from a sixth isolate showed no apparent relationship with the plasmids of the other strains.

Original languageEnglish
Pages (from-to)243-247
Number of pages5
JournalLetters in Applied Microbiology
Volume26
Issue number4
Publication statusPublished - Apr 1998

Keywords

  • DNA
  • REPLICATION
  • SEQUENCE
  • RUMEN

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