Leptin Up-Regulates Pro-Inflammatory Cytokines in Discrete Cells Within Mouse Colon

Sara Padidar, Andrew J. Farquharson, Lynda M. Williams, Eirini Kelaiditi, Nigel Hoggard, John R. Arthur, Janice E. Drew

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Dysregulation of leptin associated with obesity is implicated in obesity-related colon cancer, but mechanisms are elusive. Increased adiposity and elevated plasma leptin are associated with perturbed metabolism in colon and leptin receptors are expressed on colon epithelium. We hypothesise that obesity increases the sensitivity of the colon to cancer by disrupting leptin-regulated gene targets within colon tissues. PCR arrays were used to firstly identify leptin responsive genes and secondly to identify responses to leptin challenge in wildtype mice, or those lacking leptin (ob/ob). Leptin-regulated genes were localised in the colon using in situ hybridisation. IL6, IL1b and CXCL1 were up-regulated by leptin and localised to discrete cells in gut epithelium, lamina propria, muscularis and at the peritoneal serosal surface. Leptin regulates pro-inflammatory genes such as IL6, IL1b and CXCL1, and might increase the risk of colon cancer among obese individuals. J. Cell. Physiol. 226: 2123-2130, 2011. (C) 2010 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)2123-2130
Number of pages8
JournalJournal of Cellular Physiology
Volume226
Issue number8
Early online date25 Apr 2011
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Leptin
Colon
Up-Regulation
Cytokines
Genes
Colonic Neoplasms
Obesity
Interleukin-6
Epithelium
Leptin Receptors
Adiposity
Metabolism
In Situ Hybridization
Mucous Membrane
Tissue
Plasmas
Polymerase Chain Reaction

Keywords

  • Animals
  • Chemokine CXCL1
  • Colon
  • Cytokines
  • Gene Expression
  • Inflammation
  • Interleukin-1beta
  • Interleukin-6
  • Leptin
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Obese
  • Obesity
  • Up-Regulation

Cite this

Leptin Up-Regulates Pro-Inflammatory Cytokines in Discrete Cells Within Mouse Colon. / Padidar, Sara; Farquharson, Andrew J.; Williams, Lynda M.; Kelaiditi, Eirini; Hoggard, Nigel; Arthur, John R.; Drew, Janice E.

In: Journal of Cellular Physiology, Vol. 226, No. 8, 08.2011, p. 2123-2130.

Research output: Contribution to journalArticle

Padidar, Sara ; Farquharson, Andrew J. ; Williams, Lynda M. ; Kelaiditi, Eirini ; Hoggard, Nigel ; Arthur, John R. ; Drew, Janice E. / Leptin Up-Regulates Pro-Inflammatory Cytokines in Discrete Cells Within Mouse Colon. In: Journal of Cellular Physiology. 2011 ; Vol. 226, No. 8. pp. 2123-2130.
@article{12feebeb59b74347b47540d75ffb97d3,
title = "Leptin Up-Regulates Pro-Inflammatory Cytokines in Discrete Cells Within Mouse Colon",
abstract = "Dysregulation of leptin associated with obesity is implicated in obesity-related colon cancer, but mechanisms are elusive. Increased adiposity and elevated plasma leptin are associated with perturbed metabolism in colon and leptin receptors are expressed on colon epithelium. We hypothesise that obesity increases the sensitivity of the colon to cancer by disrupting leptin-regulated gene targets within colon tissues. PCR arrays were used to firstly identify leptin responsive genes and secondly to identify responses to leptin challenge in wildtype mice, or those lacking leptin (ob/ob). Leptin-regulated genes were localised in the colon using in situ hybridisation. IL6, IL1b and CXCL1 were up-regulated by leptin and localised to discrete cells in gut epithelium, lamina propria, muscularis and at the peritoneal serosal surface. Leptin regulates pro-inflammatory genes such as IL6, IL1b and CXCL1, and might increase the risk of colon cancer among obese individuals. J. Cell. Physiol. 226: 2123-2130, 2011. (C) 2010 Wiley-Liss, Inc.",
keywords = "Animals, Chemokine CXCL1, Colon, Cytokines, Gene Expression, Inflammation, Interleukin-1beta, Interleukin-6, Leptin, Male, Mice, Mice, Inbred C57BL, Mice, Obese, Obesity, Up-Regulation",
author = "Sara Padidar and Farquharson, {Andrew J.} and Williams, {Lynda M.} and Eirini Kelaiditi and Nigel Hoggard and Arthur, {John R.} and Drew, {Janice E.}",
note = "Copyright {\circledC} 2010 Wiley-Liss, Inc.",
year = "2011",
month = "8",
doi = "10.1002/jcp.22546",
language = "English",
volume = "226",
pages = "2123--2130",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "8",

}

TY - JOUR

T1 - Leptin Up-Regulates Pro-Inflammatory Cytokines in Discrete Cells Within Mouse Colon

AU - Padidar, Sara

AU - Farquharson, Andrew J.

AU - Williams, Lynda M.

AU - Kelaiditi, Eirini

AU - Hoggard, Nigel

AU - Arthur, John R.

AU - Drew, Janice E.

N1 - Copyright © 2010 Wiley-Liss, Inc.

PY - 2011/8

Y1 - 2011/8

N2 - Dysregulation of leptin associated with obesity is implicated in obesity-related colon cancer, but mechanisms are elusive. Increased adiposity and elevated plasma leptin are associated with perturbed metabolism in colon and leptin receptors are expressed on colon epithelium. We hypothesise that obesity increases the sensitivity of the colon to cancer by disrupting leptin-regulated gene targets within colon tissues. PCR arrays were used to firstly identify leptin responsive genes and secondly to identify responses to leptin challenge in wildtype mice, or those lacking leptin (ob/ob). Leptin-regulated genes were localised in the colon using in situ hybridisation. IL6, IL1b and CXCL1 were up-regulated by leptin and localised to discrete cells in gut epithelium, lamina propria, muscularis and at the peritoneal serosal surface. Leptin regulates pro-inflammatory genes such as IL6, IL1b and CXCL1, and might increase the risk of colon cancer among obese individuals. J. Cell. Physiol. 226: 2123-2130, 2011. (C) 2010 Wiley-Liss, Inc.

AB - Dysregulation of leptin associated with obesity is implicated in obesity-related colon cancer, but mechanisms are elusive. Increased adiposity and elevated plasma leptin are associated with perturbed metabolism in colon and leptin receptors are expressed on colon epithelium. We hypothesise that obesity increases the sensitivity of the colon to cancer by disrupting leptin-regulated gene targets within colon tissues. PCR arrays were used to firstly identify leptin responsive genes and secondly to identify responses to leptin challenge in wildtype mice, or those lacking leptin (ob/ob). Leptin-regulated genes were localised in the colon using in situ hybridisation. IL6, IL1b and CXCL1 were up-regulated by leptin and localised to discrete cells in gut epithelium, lamina propria, muscularis and at the peritoneal serosal surface. Leptin regulates pro-inflammatory genes such as IL6, IL1b and CXCL1, and might increase the risk of colon cancer among obese individuals. J. Cell. Physiol. 226: 2123-2130, 2011. (C) 2010 Wiley-Liss, Inc.

KW - Animals

KW - Chemokine CXCL1

KW - Colon

KW - Cytokines

KW - Gene Expression

KW - Inflammation

KW - Interleukin-1beta

KW - Interleukin-6

KW - Leptin

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Obese

KW - Obesity

KW - Up-Regulation

U2 - 10.1002/jcp.22546

DO - 10.1002/jcp.22546

M3 - Article

VL - 226

SP - 2123

EP - 2130

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 8

ER -