Leptin Up-Regulates Pro-Inflammatory Cytokines in Discrete Cells Within Mouse Colon

Sara Padidar, Andrew J. Farquharson, Lynda M. Williams, Eirini Kelaiditi, Nigel Hoggard, John R. Arthur, Janice E. Drew

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Dysregulation of leptin associated with obesity is implicated in obesity-related colon cancer, but mechanisms are elusive. Increased adiposity and elevated plasma leptin are associated with perturbed metabolism in colon and leptin receptors are expressed on colon epithelium. We hypothesise that obesity increases the sensitivity of the colon to cancer by disrupting leptin-regulated gene targets within colon tissues. PCR arrays were used to firstly identify leptin responsive genes and secondly to identify responses to leptin challenge in wildtype mice, or those lacking leptin (ob/ob). Leptin-regulated genes were localised in the colon using in situ hybridisation. IL6, IL1b and CXCL1 were up-regulated by leptin and localised to discrete cells in gut epithelium, lamina propria, muscularis and at the peritoneal serosal surface. Leptin regulates pro-inflammatory genes such as IL6, IL1b and CXCL1, and might increase the risk of colon cancer among obese individuals. J. Cell. Physiol. 226: 2123-2130, 2011. (C) 2010 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)2123-2130
Number of pages8
JournalJournal of Cellular Physiology
Volume226
Issue number8
Early online date25 Apr 2011
DOIs
Publication statusPublished - Aug 2011

Keywords

  • Animals
  • Chemokine CXCL1
  • Colon
  • Cytokines
  • Gene Expression
  • Inflammation
  • Interleukin-1beta
  • Interleukin-6
  • Leptin
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Obese
  • Obesity
  • Up-Regulation

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