Leukotriene B4 generation by human neutrophils following IgG-dependent stimulation

P Fitzharris, O Cromwell, R Moqbel, A Hartnell, G M Walsh, C Harvey, A B Kay

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6 Citations (Scopus)

Abstract

It has previously been shown that human neutrophils generate substantial quantities of LTB4 when stimulated with the calcium ionophore, A23187, or with unopsonized zymosan. We now report that normal human neutrophils produced substantial quantities of LTB4 (measured by radioimmunoassay and validated by RP-HPLC) when incubated with large non-phagocytosable IgG-coated beads (Sepharose 4B). LTB4 was identified in both the extra and intracellular compartments. The production of LTB4 was dependent upon the number of IgG-coated particles and the concentration of IgG bound to the beads. Release was maximal after a 15-30 min incubation time and was enhanced by prior activation of the neutrophils with the synthetic bacterial product f-met-leu-phe. Comparable LTB4 production was also observed when neutrophils were incubated with antigen (Aspergillus fumigatus)-coated beads sensitized with purified IgG obtained from the sera of patients with allergic bronchopulmonary aspergillosis. These results suggest a further mechanism by which neutrophils may be activated to produce inflammatory mediators in the tissues.
Original languageEnglish
Pages (from-to)449-455
Number of pages7
JournalImmunology
Volume61
Issue number4
Publication statusPublished - Aug 1987

Cite this

Fitzharris, P., Cromwell, O., Moqbel, R., Hartnell, A., Walsh, G. M., Harvey, C., & Kay, A. B. (1987). Leukotriene B4 generation by human neutrophils following IgG-dependent stimulation. Immunology, 61(4), 449-455.

Leukotriene B4 generation by human neutrophils following IgG-dependent stimulation. / Fitzharris, P ; Cromwell, O ; Moqbel, R ; Hartnell, A ; Walsh, G M; Harvey, C ; Kay, A B.

In: Immunology, Vol. 61, No. 4, 08.1987, p. 449-455.

Research output: Contribution to journalArticle

Fitzharris, P, Cromwell, O, Moqbel, R, Hartnell, A, Walsh, GM, Harvey, C & Kay, AB 1987, 'Leukotriene B4 generation by human neutrophils following IgG-dependent stimulation', Immunology, vol. 61, no. 4, pp. 449-455.
Fitzharris P, Cromwell O, Moqbel R, Hartnell A, Walsh GM, Harvey C et al. Leukotriene B4 generation by human neutrophils following IgG-dependent stimulation. Immunology. 1987 Aug;61(4):449-455.
Fitzharris, P ; Cromwell, O ; Moqbel, R ; Hartnell, A ; Walsh, G M ; Harvey, C ; Kay, A B. / Leukotriene B4 generation by human neutrophils following IgG-dependent stimulation. In: Immunology. 1987 ; Vol. 61, No. 4. pp. 449-455.
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N2 - It has previously been shown that human neutrophils generate substantial quantities of LTB4 when stimulated with the calcium ionophore, A23187, or with unopsonized zymosan. We now report that normal human neutrophils produced substantial quantities of LTB4 (measured by radioimmunoassay and validated by RP-HPLC) when incubated with large non-phagocytosable IgG-coated beads (Sepharose 4B). LTB4 was identified in both the extra and intracellular compartments. The production of LTB4 was dependent upon the number of IgG-coated particles and the concentration of IgG bound to the beads. Release was maximal after a 15-30 min incubation time and was enhanced by prior activation of the neutrophils with the synthetic bacterial product f-met-leu-phe. Comparable LTB4 production was also observed when neutrophils were incubated with antigen (Aspergillus fumigatus)-coated beads sensitized with purified IgG obtained from the sera of patients with allergic bronchopulmonary aspergillosis. These results suggest a further mechanism by which neutrophils may be activated to produce inflammatory mediators in the tissues.

AB - It has previously been shown that human neutrophils generate substantial quantities of LTB4 when stimulated with the calcium ionophore, A23187, or with unopsonized zymosan. We now report that normal human neutrophils produced substantial quantities of LTB4 (measured by radioimmunoassay and validated by RP-HPLC) when incubated with large non-phagocytosable IgG-coated beads (Sepharose 4B). LTB4 was identified in both the extra and intracellular compartments. The production of LTB4 was dependent upon the number of IgG-coated particles and the concentration of IgG bound to the beads. Release was maximal after a 15-30 min incubation time and was enhanced by prior activation of the neutrophils with the synthetic bacterial product f-met-leu-phe. Comparable LTB4 production was also observed when neutrophils were incubated with antigen (Aspergillus fumigatus)-coated beads sensitized with purified IgG obtained from the sera of patients with allergic bronchopulmonary aspergillosis. These results suggest a further mechanism by which neutrophils may be activated to produce inflammatory mediators in the tissues.

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