Limits to sustained energy intake. XXI. Effect of exposing the mother, but not her pups, to a cold environment during lactation in mice

Teresa G Valencak, Paul Wright, Ashleigh Weir, Sharon Elizabeth Mitchell, Lobke M Vaanholt, Catherine Hambly, Elzbieta Król, John R Speakman

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Abstract

The capacity of females to dissipate heat may constrain sustained energy intake during lactation. However, some previous experiments supporting this concept have confounded the impact of temperature on the mothers with the impact on the pups. We aimed to separate these effects in lactating laboratory mice (MF1 strain) by giving the mothers access to cages at two ambient temperatures (10 and 21°C) joined by a tube. Food was available only in the cold cage, but females could also choose go to this cage to cool down while their pups were housed in the warmer cage. Control animals had access to the same configuration of cages but with both maintained at 21°C. We hypothesised that if females were limited by heat dissipation, alleviating the heat load by providing a cool environment would allow them to dissipate more heat, take in more food, generate more milk and hence wean heavier litters. We measured maternal energy budgets and monitored time courses of core body temperature and physical activity. To minimise the variance in energy budgets, all litters were adjusted to 12 (±1) pups. Females in the experimental group had higher energy intake (F1,14=15.8, P=0.0014) and higher assimilated energy (F1,13=10.7, P=0.006), and provided their pups with more milk (F1,13=6.65, P=0.03), consistent with the heat dissipation limit theory. Yet, despite keeping demand constant, mean pup growth rates were similar (F1,13=0.06, P=0.8); thus, our data emphasise the difficulties of inferring milk production indirectly from pup growth.

Original languageEnglish
Pages (from-to)4326-4333
Number of pages8
JournalJournal of Experimental Biology
Volume216
Issue number23
DOIs
Publication statusPublished - 1 Dec 2013

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lactation
Energy Intake
Lactation
pups
energy intake
Hot Temperature
cages
mice
heat
twenty first century
energy budget
Milk
milk
energy
dissipation
Budgets
litter
litters (young animals)
milk production
physical activity

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Limits to sustained energy intake. XXI. Effect of exposing the mother, but not her pups, to a cold environment during lactation in mice. / Valencak, Teresa G; Wright, Paul; Weir, Ashleigh; Mitchell, Sharon Elizabeth; Vaanholt, Lobke M; Hambly, Catherine; Król, Elzbieta; Speakman, John R.

In: Journal of Experimental Biology, Vol. 216, No. 23, 01.12.2013, p. 4326-4333.

Research output: Contribution to journalArticle

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