Lineage/species-specific expansion of the Mx gene family in teleosts: Differential expression and modulation of nine Mx genes in rainbow trout Oncorhynchus mykiss

Tingyu Wang, Fuguo Liu, Guangming Tian, Christopher J. Secombes (Corresponding Author), Tiehui Wang (Corresponding Author)

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Myxovirus resistance (Mx) proteins are interferon (IFN)-inducible Dynamin-like GTPases, which play an important role in antiviral immunity. Three Mx genes (Mx1-3) have been cloned previously in rainbow trout. In this study, an additional six Mx genes were cloned that reside in four chromosomal loci. Further bioinformatics analysis suggests the presence of three teleost Mx groups (TMG) each with a characteristic gene organisation. Salmonid Mx belong to TMG1 and TMG2. The increased salmonid Mx gene copies are due mainly to local gene duplications that happened before and after salmonid speciation, in a lineage/species specific manner. Trout Mx molecules have been diversified in the loop 1 and 4 regions, and in the nuclear localisation signal in loop 4. The trout Mx genes were shown to be differentially expressed in tissues, with high levels of expression of TMG1 (Mx1-4) in blood and TMG2 (Mx5-9) in intestine. The expression of the majority of the trout Mx genes was induced by poly IC in vitro and in vivo, and increased during development. In addition, induction by antiviral (IFN) and proinflammatory cytokines was studied, and showed that type I IFN, IFNγ and IL-1β can induce Mx gene expression in an Mx gene-, cytokine- and cell line-dependent manner. These results show that salmonids possess a large number Mx genes as well as complex regulatory pathways, which may contribute to their success in an anadromous life style.
Original languageEnglish
Pages (from-to)413-430
Number of pages18
JournalFish & Shellfish Immunology
Volume90
Early online date4 May 2019
DOIs
Publication statusPublished - 1 Jul 2019

Fingerprint

teleost
rainbow
Oncorhynchus mykiss
Genes
Modulation
gene
interferons
genes
salmonid
trout
Interferons
cytokines
Antiviral Agents
Myxovirus Resistance Proteins
polyinosinic-polycytidylic acid
nuclear localization signals
Cytokines
Dynamins
family
Interferon Type I

Keywords

  • Rainbow trout
  • Mx
  • Anti-viral defence
  • Evolution
  • Gene expression
  • Modulation
  • Type I interferon
  • IFNγ
  • IL-1β

Cite this

Lineage/species-specific expansion of the Mx gene family in teleosts : Differential expression and modulation of nine Mx genes in rainbow trout Oncorhynchus mykiss. / Wang, Tingyu; Liu, Fuguo; Tian, Guangming; Secombes, Christopher J. (Corresponding Author); Wang, Tiehui (Corresponding Author).

In: Fish & Shellfish Immunology, Vol. 90, 01.07.2019, p. 413-430.

Research output: Contribution to journalArticle

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abstract = "Myxovirus resistance (Mx) proteins are interferon (IFN)-inducible Dynamin-like GTPases, which play an important role in antiviral immunity. Three Mx genes (Mx1-3) have been cloned previously in rainbow trout. In this study, an additional six Mx genes were cloned that reside in four chromosomal loci. Further bioinformatics analysis suggests the presence of three teleost Mx groups (TMG) each with a characteristic gene organisation. Salmonid Mx belong to TMG1 and TMG2. The increased salmonid Mx gene copies are due mainly to local gene duplications that happened before and after salmonid speciation, in a lineage/species specific manner. Trout Mx molecules have been diversified in the loop 1 and 4 regions, and in the nuclear localisation signal in loop 4. The trout Mx genes were shown to be differentially expressed in tissues, with high levels of expression of TMG1 (Mx1-4) in blood and TMG2 (Mx5-9) in intestine. The expression of the majority of the trout Mx genes was induced by poly IC in vitro and in vivo, and increased during development. In addition, induction by antiviral (IFN) and proinflammatory cytokines was studied, and showed that type I IFN, IFNγ and IL-1β can induce Mx gene expression in an Mx gene-, cytokine- and cell line-dependent manner. These results show that salmonids possess a large number Mx genes as well as complex regulatory pathways, which may contribute to their success in an anadromous life style.",
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author = "Tingyu Wang and Fuguo Liu and Guangming Tian and Secombes, {Christopher J.} and Tiehui Wang",
note = "This work was partially funded by the Biotechnology and Biological Sciences Research Council (BBSRC, BB/R008442/1). Tingyu Wang was supported by the Ministry of Science and Technology, Republic of China (Taiwan) (MOST 107-2917-I-564-019). Fuguo Liu was supported by a Newton International Fellowship funded by the Academy of Medical Sciences, UK (AMS, NIF004\1036). Guangming Tian was supported financially by the State Scholarship Fund organised by the China Scholarship Council (201808420042).",
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