Links between methane flux and transcriptional activities of methanogens and methane oxidizers in a blanket peat bog

Thomas E Freitag, Sylvia Toet, Phil Ineson, James I Prosser

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The relationship between biogeochemical process rates and microbial functional activity was investigated by analysis of the transcriptional dynamics of the key functional genes for methanogenesis (methyl coenzyme M reductase; mcrA) and methane oxidation (particulate methane monooxygenase; pmoA) and in situ methane flux at two peat soil field sites with contrasting net methane-emitting and -oxidizing characteristics. qPCR was used to quantify the abundances of mcrA and pmoA genes and transcripts at two soil depths. Total methanogen and methanotroph transcriptional dynamics, calculated from mcrA and pmoA gene : transcript abundance ratios, were similar at both sites and depths. However, a linear relationship was demonstrated between surface mcrA and pmoA transcript dynamics and surface flux rates at the methane-emitting and methane-oxidizing sites, respectively. Results indicate that methanotroph activity was at least partially substrate-limited at the methane-emitting site and by other factors at the methane-oxidizing site. Soil depth also contributed to the control of surface methane fluxes, but to a lesser extent. Small differences in the soil water content may have contributed to differences in methanogen and methanotroph activities. This study therefore provides a first insight into the regulation of in situ, field-level surface CH(4) flux at the molecular level by an accurate reflection of gene : transcript abundance ratios for the key genes in methane generation and consumption.
Original languageEnglish
Pages (from-to)157-165
Number of pages9
JournalFEMS Microbiology Ecology
Volume73
Issue number1
Early online date30 Mar 2010
DOIs
Publication statusPublished - Jul 2010

Fingerprint

Wetlands
Methane
peatland
Soil
methane
gene
Genes
methane monooxygenase
soil depth
peat soil
methanogenesis
surface flux
soil water
water content
Water
oxidation
substrate

Keywords

  • archaea
  • bacteria
  • genes, archaeal
  • genes, bacterial
  • methane
  • oxidation-reduction
  • oxidoreductases
  • oxygenases
  • soil
  • soil microbiology
  • wetlands

Cite this

Links between methane flux and transcriptional activities of methanogens and methane oxidizers in a blanket peat bog. / Freitag, Thomas E; Toet, Sylvia; Ineson, Phil; Prosser, James I.

In: FEMS Microbiology Ecology, Vol. 73, No. 1, 07.2010, p. 157-165.

Research output: Contribution to journalArticle

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